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Citations for abcam :
2101 - 2150 of 2341 citations for Human Chemokine Like Factor Superfamily 6 CKLFSF6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The protein was transferred overnight to 0.22µ PVDF membranes and probed using either anti-HA (Abcam Cat# ab9110, RRID:AB_307019) or anti-Myc (Abcam Cat# ab32 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight in primary antibodies against green- fluorescent protein (GFP, 1:1000 in antibody diluent, Abcam, ab13970), calcitonin gene-related peptide (CGRP ...
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bioRxiv - Neuroscience 2023Quote: ... Blockade of IL-1R was performed using the recombinant mouse IL1-RA protein (1-1000 mg/mL, abcam #ab283475).
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: Protein was visualized by SDS-PAGE analysis using 4-12% bis-tris mPAGE gels and stained using InstantBlue (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450) and a 1:5000 dilution of donkey anti-goat IRDye800CW (Licor 926-32214) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Molecular Biology 2021Quote: Expression of GLIS1-βGAL fusion protein was examined by staining tissue sections with chicken anti-βGAL (1:500, ab9361, Abcam) and Alexa Fluor@ 488 donkey anti-chicken IgG (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Physiology 2019Quote: ... rabbit acetylated SOD2 (K122), rabbit SOD2, and rabbit OXPHOS cocktail (to analyze NDUFB8, SDHB and UQCR2 protein content) from Abcam. Fluorescent anti-mouse or anti-rabbit secondary antibodies (red ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Plant Biology 2019Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse postsynaptic density protein 95 (PSD-95, 1:1000 for cells, 1:200 for tissues, Abcam, catalog ab99009), rabbit anti-mouse vesicular gamma aminobutyric acid (GABA ...
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bioRxiv - Microbiology 2021Quote: ... PBS for 45 minutes and further incubated in blocking solution containing anti-SARS-CoV-2 spike protein subunit 2 (Abcam) 1:1000 dilution for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The molecular masses of the protein bands were assessed by comparing with the mass of the bands in the lane containing the standards (Abcam). The experiment was performed in triplicate and the bands corresponding to the heterodimer and heterotetramer were visualized in all the three experiments.
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919; goat anti-EHD2, 1:200, Abcam 23935 ...
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bioRxiv - Biophysics 2022Quote: ... 1:1000 dilution in blocking buffer) plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919 ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Immunology 2021Quote: ... AD-01 (NH2-QIRQQPRDPPTETLELEVSPDPAS-OH) (Almac Group, United Kingdom), ALM201 (NH2-IRQQPRDPPTETLELEVSPDPAS-OH) (Almac group, United Kingdom) and recombinant FKBPL protein (ab98117; Abcam). All experiments were carried out at 37°C in a humidified atmosphere of 95% O2/5% CO2.
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bioRxiv - Microbiology 2020Quote: ... Co-IP for NPC1 was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against NPC1 (Abcam, ab108921). The cell pellets were incubated for 30 minutes on ice with 200μl lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then mixed with either anti-HA magnetic beads (Thermoscientific) or protein G magnetic beads (Thermoscientific) coupled with anti-pADPr (Abcam) (beads were pre-blocked with 1% BSA) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were permeabilized with 0.1% Triton X 100 in 1X PBS for 5 min and blocked with Protein Block (Abcam, USA) for 20 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: PPGYFP NTS tissue was processed free-floating and incubated with chicken anti-green fluorescent protein (GFP; AbCam, ab13970, 1:1000) and/or rabbit anti-c-FOS (Calbiochem ...
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bioRxiv - Immunology 2022Quote: ... were washed with lysis buffer (containing protein inhibitors) and incubated with 5 µg of 4C4 antibody or IgG1k as a control isotype (ab18443 Abcam) at 4°C overnight under rotation ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Molecular Biology 2019Quote: ... The HA-tagged proteins and Histone H3 were detected with anti-HA (Abcam # ab9110; 1:1000 in 1% milk-PBST) and anti-H3 (Abcam # ab1791 ...
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bioRxiv - Plant Biology 2019Quote: ... Protein detection was performed using a monoclonal rabbit anti-VEGF primary antibody (1:1000 dilution, ab52917, Abcam plc, Cambridge, UK) and a goat anti-rabbit HRP secondary antibody (1:5000 ...