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Citations for abcam :
2051 - 2100 of 2180 citations for Recombinant Human GAB1 Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... anti-voltage-dependent anion-selective channel protein 1 (VDAC1)/porin (mouse monoclonal, #ab14734, RRID: AB_443084, Abcam; 1:2000 for WB), anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The co-immunoprecipitated and immunoprecipitated proteins were then characterized by Western Blotting using anti-HA monoclonal antibody (ab18181, Abcam, USA) and anti-myc monoclonal antibody (ab18185 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Our antibody against DARPP-32 binds to a 16 AA segment on a region of the protein that does not contain any phosphorylation sites (Abcam ab40801) and therefore it labels DARPP-32 irrespective of phosphorylation state and cellular location ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4 °C with Protein G magnetic beads (Dynabeads: ThemoFisher) that had been pre-incubated with anti-H3K27Ac (Abcam, ab4729) or anti-Sox2 (R&D ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Genetics 2020Quote: ... Reactions were stopped by addition of 6x SDS Laemmli buffer and proteins were resolved by 15% SDS-PAGE and analysed by western blotting with anti-ubiquitin antibodies (1:2000 Abcam P4D1) and anti-Rad51 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The signal intensity from the examined proteins were evaluated on free-floating sections using the standard method of simultaneously combined staining (Abcam Protocol). Brain slices (50 μm ...
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bioRxiv - Neuroscience 2020Quote: ... at 100 μm thickness (coronal) and processed for free floating immunofluorescence using chicken anti Green-Fluorescent-Protein (GFP) primary antibody (1:1000 AbCam, ab13970), and Goat anti Chicken-alexa488 secondary antibody (1:500 thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... were resolved by SDS-PAGE and the target proteins were detected with Western blot (WB) using the antibodies as follows: GAPDH Rabbit monoclonal antibody (ab181602, Abcam, UK), SLC40A1 Rabbit polyclonal antibody (ab58695 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: Growth factor proteins were analysed in conditioned medium and dissociative tissue extracts by Western blotting using anti-HDGF from AbCam (ab131064), anti-CTGF from Santa Cruz (sc-14939 ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin levels were determined in duplicate using 150 μg of protein lysates per well using a sandwich ELISA assay (abcam, ab252364). Western blot and qPCR analyses were carried out as described above ...
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bioRxiv - Microbiology 2022Quote: ... Spain) binding to the 229E nucleocapsid protein and a goat anti-mouse secondary antibody conjugated with fluorescein isothiocyanate (Abcam, United Kingdom). TCID50 titers were calculated according to the method of Reed and Muench (44).
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bioRxiv - Cell Biology 2022Quote: ... Chromatin immunoprecipitation was carried out using Protein A Dynabeads conjugated to approximately 1 μg of S139 rabbit γ-H2AX (Abcam 29893) or Rabbit H2AX (Merck Millipore 07627 ...
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bioRxiv - Cancer Biology 2021Quote: Protein extraction and immunoblotting was performed according to standard procedures with primary antibodies against FLI1 (1:500, rabbit, ab15289, abcam, UK) and GAPDH (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... deparaffinized sections were rehydrated and stained using specific antibodies against Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5, ab219107, Abcam, USA), Lysozyme (ab76784 ...
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bioRxiv - Developmental Biology 2019Quote: Phospho-protein staining was performed as previously described (Okuda et al. 2018) using the following antibodies: GFP (abcam cat. no. ab13970), pErk1/2 (cell signaling technologies cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Whole pancreas protein extracts were isolated and incubated with mouse inflammation/cytokine antibody arrays (40 targets, Abcam, ab133999; 97 targets, Abcam ab169820), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins of interests were detected using the following primary antibodies: anti-rabbit Catalase (1:375; catalog #Ab52477; Abcam, Cambridge, MA); anti-rabbit Glutathione Peroxidase (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Neuroscience 2021Quote: ... constant shaking) with the following primary antibodies: sheep anti-phospo-S6 ribosomal protein (pS6; 1:250, ab65748, Abcam, Cambridge, Massachusetts, USA); mouse anti-D3R (1:150 ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 hour then incubated overnight with Anti-Amyloid beta precursor protein (Y188) rabbit monoclonal antibody at 1:1,000 (Abcam Cat# ab32136) and Anti-VCP mouse monoclonal antibody at 1:2,000 (Abcam Cat# ab11433) ...
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bioRxiv - Microbiology 2020Quote: ... or IgG were used to immunoprecipitate the chromatin fragments in the supernatant by overnight incubation using protein A sepharose beads (Abcam, UK) at 4 °C.The beads were washed twice with wash buffer ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Cancer Biology 2020Quote: S-nitrosylation of proteins was detected by western blot using an S-nitrosylation specific antibody (HY8E12 clone, Abcam # 94930, 1:20) or the Pierce S-Nitrosylation Western Blot detection kit (Thermo Fisher Scientific # 90105) ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The slides were incubated with primary rabbit anti-SARS nucleocapsid protein antibody(NB100-56576, Novus, USA) overnight at 4°C and then with goat anti-rabbit IgG HRP (Abcam, UK) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed at least three times with PBST and incubated with native Streptavidin protein DyLight 488 (1:750 dilution; Abcam, ab134349) in 0.5% fish skin gelatin with PBST for 2 hours ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... NF200 (1:500, Chemicon International, Temecula, CA, USA) rabbit monoclonal amyloid precursor protein (APP) antibody [Y188] (1:500, Abcam, Cambridge, MA) and NeuN (1:500 ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with antibodies for one hour at 4°C in an overhead tumbler before immune complexes were retrieved by protein G (GE Healthcare or abcam) or A Sepharose (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was transferred to a fresh Eppendorf tube and the protein concentration was assessed using Bradford reagent (Abcam, cat#ab119216) and stored at -80°C when not used immediately ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Molecular Biology 2023Quote: ... were coupled with 10μg of anti-protein of interest (POI; anti-Foxg1 ChIP-grade, rabbit polyclonal, Abcam #ab18259; anti-GFP, rabbit polyclonal, Abcam #ab290), or 10μg of rabbit IgG (Millipore #12370 ...
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bioRxiv - Physiology 2023Quote: ... Frozen sections were fixed with fresh 4% paraformaldehyde and neutrophil elastase protein was detected using anti-neutrophil elastase antibody (Abcam #68672).