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Citations for abcam :
2051 - 2100 of 2305 citations for Mayaro Virus E2 Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and the obtained values represent the ratio between the immunodetected protein and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Abcam) loading control ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then incubated with a 10-fold molar excess of the chosen guanine nucleotide (GMPPNP or GDP (AbCam)) for 3 hours at room temperature (22 °C ...
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bioRxiv - Cell Biology 2021Quote: ... as determined by the staining of the SDS-PAGE gels with CBBR (InstantBlue Coomassie Protein Stain [ISB1L], Abcam: ab119211). Aliquots (10 μl ...
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bioRxiv - Cell Biology 2019Quote: Cytosolic and nuclear proteins were extracted from cultured NRVMs using the Nuclear Extraction Kit (Catalog. #: NC113474; Abcam, Cambridge, MA) according to the manual provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... patient serum was added to a 96-well MULTI-ARRAY plate (Meso Scale Discovery) coated with CD40 protein (Abcam) and allowed to bind at room temp for 2hr ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226, Abcam) for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was used to visualize co-expression of certain proteins in glial or neuronal cell types: C3(Abcam 181147) and S100β (Abcam 41428) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA-tagged proteins and histone H3 were detected with anti-HA (Abcam ab9110, 1:1,000 in 1% milk-PBST) and anti-H3 (Abcam ab1791 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in primary antibodies against the first protein of interest and tubulin (Abcam, Cambridge, UK,; 1:5000) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... IHC staining was applied to examine the expression of the protein encoded by Mpo (Myeloperoxidase, 1:1000, ab208670, Abcam) and Il1b (IL-1 beta ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were probed in Can Get Signal Solution1 (Toyobo) using following primary antibodies: anti-Gpx4 (1:10,000, Abcam, ab125066), anti-β-actin (1:20,000 ...
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bioRxiv - Genomics 2024Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were washed once with distilled water and stained with InstantBlue® Coomassie Protein Stain (Abcam, Cambridge, UK). The gels were viewed using a ChemiDOC XRS molecular imager (Biorad ...
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bioRxiv - Immunology 2023Quote: ... Obtained protein fraction has been subjected to Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... or mouse antibodies recognizing cellular proteins (anti-EEA1, BD Biosciences, 610457, 1:100; anti-VPS35, Abcam, ab57632, 1:200); rabbit antibodies recognizing cellular proteins (anti-TGN46 ...
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bioRxiv - Genomics 2023Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was transferred overnight to 0.22µ PVDF membranes and probed using either anti-HA (Abcam Cat# ab9110, RRID:AB_307019) or anti-Myc (Abcam Cat# ab32 ...
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bioRxiv - Biochemistry 2022Quote: ... and the quality of the purification was assessed via SDS-PAGE followed by InstantBlue Coomassie protein stain (abcam: ab119211) staining and immunoblotting ...
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bioRxiv - Biochemistry 2023Quote: ... Then gels were either stained with Coomassie-blue (15 µg of protein per lane) (Instant blue, abcam, Cambridge, UK) or transferred to nitrocellulose membranes (0.25 µg or 1 µg of protein per lane for the monomeric transporters and heterodimer respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Blockade of IL-1R was performed using the recombinant mouse IL1-RA protein (1-1000 mg/mL, abcam #ab283475).
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight in primary antibodies against green- fluorescent protein (GFP, 1:1000 in antibody diluent, Abcam, ab13970), calcitonin gene-related peptide (CGRP ...
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bioRxiv - Molecular Biology 2023Quote: ... In each capillary 0.2mg/mL protein was loaded for analysis with antibodies to dystrophin (Abcam # ab15277, dilution 1:15) as in 26 ...
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: Protein was visualized by SDS-PAGE analysis using 4-12% bis-tris mPAGE gels and stained using InstantBlue (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450) and a 1:5000 dilution of donkey anti-goat IRDye800CW (Licor 926-32214) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Molecular Biology 2021Quote: Expression of GLIS1-βGAL fusion protein was examined by staining tissue sections with chicken anti-βGAL (1:500, ab9361, Abcam) and Alexa Fluor@ 488 donkey anti-chicken IgG (1:2000 ...
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bioRxiv - Physiology 2019Quote: ... rabbit acetylated SOD2 (K122), rabbit SOD2, and rabbit OXPHOS cocktail (to analyze NDUFB8, SDHB and UQCR2 protein content) from Abcam. Fluorescent anti-mouse or anti-rabbit secondary antibodies (red ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Plant Biology 2019Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse postsynaptic density protein 95 (PSD-95, 1:1000 for cells, 1:200 for tissues, Abcam, catalog ab99009), rabbit anti-mouse vesicular gamma aminobutyric acid (GABA ...
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bioRxiv - Microbiology 2021Quote: ... PBS for 45 minutes and further incubated in blocking solution containing anti-SARS-CoV-2 spike protein subunit 2 (Abcam) 1:1000 dilution for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...