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Citations for abcam :
2001 - 2050 of 10000+ citations for Pig Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... Blots were incubated overnight in rabbit anti-mouse primary antibody for proteins of interest (Supplemental Table S4) and with horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (ab6721, Abcam). Proteins of interest were detected by incubating blots in ClarityTM Western ECL Blotting Substrate (1705061 ...
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bioRxiv - Molecular Biology 2019Quote: ... primary rabbit raised antibodies against pCRKL Y207 (37 kDa) and pCRK Y221 (34 kDa) (another downstream target protein) were procured from Abcam plc ...
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bioRxiv - Genomics 2021Quote: ... Chromatin immunoprecipitation was performed by incubating the pre-cleared chromatin with 75 μL Dynabeads Protein G bound with 7.5 μg H3K27ac antibody (Abcam ab4729) overnight at 4°C rotating ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 infected cells were visualized using SARS-CoV-2 S-protein specific immunochemistry staining with anti-SARS-CoV-2 spike glycoprotein S1 antibody (Abcam) 90.
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against S14 phosphorylated Syx (Abcam; ab63571), using the ECL detection system (Pierce Protein Research Products ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against Syx phospho-S14 (Abcam; ab63571). Digitized scans were derived by PhosphorImager (Molecular Dynamics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mCherry was identified based on its auto fluorescent signal while the luciferase protein was immuno-stained with anti-firefly luciferase antibody (Abcam) labeled with Zenon AF647 Mouse Labeling Kit (Life Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were validated for GPC1/5 knockouts via Western analysis following heparinase III digestion using a monoclonal antibody (3G10) direct against the glycosylation attachment site in the core protein structure (370260, abcam) or via sequencing (GPC1 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated for 2 hours with: (i) chicken polyclonal antibody anti microtubule-associated-protein 2 (MAP-2) (Abcam, #Ab5392) at dilution of 1/1000 in PBS containing 1% fetal calf serum and 0.1% of saponin (this antibody stains specifically cell bodies and neurites ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates containing 200 µg of total protein were incubated with anti-ERK (CST) or anti-DUSP6 (Abcam, Cambridge, UK) antibody at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-G3BP1 beads were generated by incubating 200 μL Protein-G beads with 20 μL anti-G3BP1 antibody (Abcam, ab290) in 1 mL Buffer B (200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Molecular Biology 2022Quote: ... The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211) because detection via our anti-DnaD antibody (Eurogentec ...
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: Concentrations of plasma LPS-binding protein (LBP) were examined via Mouse LBP SimpleStep ELISA according to the manufacturer’s protocol (Abcam, #ab269542). Plasma samples were prepared at a 1:16,000 dilution ...
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bioRxiv - Systems Biology 2023Quote: ... The protein expression and solubility were evaluated by both Coomassie stain and western blot (WB) of the GST and MBP fusion proteins using anti-GST (abcam) and anti-MBP (NEB ...
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bioRxiv - Genetics 2023Quote: ... and Atg18 knockdown flies with the expression of mCherry-Atg8a fusion protein were counterstained with diluted CytoPainter Phalloidin-iFlor 488 (from Abcam) for F- actin at room temperature for 15 minutes before the tissues were mounted in phosphate-buffered saline solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Percent purity of each sample was determined by running diluted aliquots of each purified protein on a 4-12% Bis-Tris gel and staining with Optiblot Blue (Abcam). After destaining ...
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Neuroscience 2023Quote: ... cells were stained for 10 minutes at 37°C with a chicken anti-green fluorescent protein (GFP) antibody (cross-reactive with YFP; ab13970, AbCam) diluted 1:1000 in neurobasal medium to stain for surface GluN2B ...
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bioRxiv - Cell Biology 2023Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and size of the protein has been evaluated by Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The co-immunoprecipitated and immunoprecipitated proteins were then characterized by Western Blotting using anti-HA monoclonal antibody (ab18181, Abcam, USA) and anti-myc monoclonal antibody (ab18185 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Developmental Biology 2020Quote: ... Loading controls were detected using antibodies against: 1:2000 Mitofusin 1 (Abcam Ab57602), 1:20,000 β-actin (Invitrogen Cat-15) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-NeuN (Chemicon, 1:1000, chicken anti-gephyrin (Abcam, USA; 1:500). Then ...
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bioRxiv - Microbiology 2020Quote: ... and anti-Furin (1:100 dilution; rabbit monoclonal, 1 Abcam, ab183495; Cambridge, UK) antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-pMad (1: 200) and rabbit anti-GFP (1:10,000) from Abcam, guinea pig anti-Traffic jam (1:10,000 ...
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bioRxiv - Neuroscience 2019Quote: ... We also locally applied 1 mM EBIO (1-Ethyl-2-benzimidazolinone; Abcam, MA), a SK channel agonist near the brain slice by pressure injection (Ellis et al. ...
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bioRxiv - Cell Biology 2019Quote: ... The following primary antibodies were used: rabbit anti-Tuj-1 (Abcam, 1:1000) for neurons ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 μl of 1 mg/ml aprotinin (activation buffer; #Ab146286, Abcam MA, USA) was added to inactivate TPCK-trypsin.
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bioRxiv - Bioengineering 2019Quote: ... The goat anti-rabbit IgG-HRP (Abcam, ab6721, 1:1500 in 1 % NGS) was added after washing and quenching of the endogenous peroxidase activity (1 % H2O2 in water for 20 min ...
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bioRxiv - Genetics 2021Quote: ... rabbit anti-β-catenin (1:300; 1 hour at room temperature; Abcam, ab32572). For signal detection ...