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Citations for abcam :
1951 - 2000 of 10000+ citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The membrane was then washed 3×15 minutes in PBST and then incubated in secondary antibody solution (PBST + 5% BSA + Goat a-Rabbit-HRP 1:5000) (Abcam, ab205718) for one hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... blocked with 5% goat serum and incubated overnight with primary polyclonal rabbit antibodies in 5% goat serum at 1:150 dilution for types I (Abcam AB34710), II (Abcam AB34712) ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were blocked with 5% BSA in PBS for 1 hr at room temperature and incubated with rabbit anti-mouse Ki67 primary antibody (Abcam ab15580) at 1:200 dilution overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue samples were washed with PBS three times for 5 minutes each before adding a secondary goat anti-rabbit IgG H & L (1:1000, Alexa Fluor® 555, Abcam) and Goat Anti-Rat IgG H & L (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were sectioned in the sagittal plane to a thickness of 5 μm prior to immunoperoxidase staining for Nestin-1 (Abcam, ab237036), a marker that is used to detect vessels in AOM-induced tumors41,42.
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bioRxiv - Immunology 2020Quote: ... The transferrin levels in cells were tested by Western blotting using anti-transferrin antibody (1:5□000 dilution, ab82411, Abcam, USA) as described above ...
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bioRxiv - Cell Biology 2019Quote: ... embryos and cells were blocked at room temperature in 5% BSA in PBS for 1 hour and incubated with primary antibodies (hCGβ, Abcam, ab9582; TBX3, Abcam, ab99302) in blocking solution overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... which was blocked with 5% (w/v) skimmed milk powder in PBS and probed for ERα at a concentration of 1:1000 (Abcam ab108398) and PRβ at a concentration of 1:1000 (Abcam ab46535 ...
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bioRxiv - Physiology 2024Quote: ... antibody kindly gifted by Jan Nedergaard and Barbara Cannon) and anti-αTubulin to control for equal loading (1:1000 solution in 5% milk in TBS-T, Abcam 2144). The following day ...
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bioRxiv - Neuroscience 2023Quote: ... then the brain slides incubated in a secondary antibody for 90 min protected from light at room temperature (0.1% TritonX-100, 5% normal goat serum, PBS solution with 1:500 goat anti-rabbit Alexa 488; Abcam, Cambridge, MA). Slides were washed again as described above ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed in PBS three times (5 min each time) and incubated with a donkey-anti-rabbit Alexa488 secondary antibody (1:1000, Abcam, USA) in PBST for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and next incubated for 1 hour in a solution of PB with 5% normal goat serum (Abcam Cat# ab7481, RRID: AB_2716553). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... ready to use DISCOVERY OmniMap anti-Rb HRP (760-4311) for PHOX2B and Phospho-SMAD-1/5/9 or rabbit anti-mouse secondary (Abcam, ab133469) followed by DISCOVERY OmniMap anti-Rb HRP (760-4311 ...
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bioRxiv - Physiology 2024Quote: ... Frozen brains were sliced into 7um and incubated in 0.1%PBST + 5%BSA with anti-Iba1 antibody (diluted 1:300, Abcam; Cat#ab178846) and/or anti-GFP antibody (diluted 1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... brains and proboscis were washed 5 times for 10 minutes with PB + 0.3% Triton X- 100 (PBT) and blocked for 1 hour in PBT + 5% normal goad serum (NGS) (Abcam, ref. ab7481). Later ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were incubated with primary antibodies overnight at 4°C (rabbit-NeuN, 1:1’000, abcam 177487; mouse-TH; 1:1’000, Immunostar 22941; chicken-GFAP, 1:1’500, abcam ab95231 ...
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bioRxiv - Cell Biology 2023Quote: ... The required primary antibody dilutions (GLI1, 1:200; DNMT1, 1:200; DNMT3a, 1:200; Abcam, Cambridge, UK) were prepared ...
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bioRxiv - Cell Biology 2020Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Plant Biology 2021Quote: ... 2% was removed as input and samples were incubated overnight with 1% antibody (for histone ChIP: anti-H3 (abcam ab1791), anti-H3K27me3 (EMD Millipore 07-449) ...
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bioRxiv - Microbiology 2020Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with mouse monoclonal anti-MBL (Biotin) (2 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam) and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Neuroscience 2022Quote: Slides were similarly processed for BDNF with the exception that slides were blocked with 10% sheep serum in 5% Triton-X PBS under coverwells in a humid chamber for 2 hours and slides were incubated with primary antibodies for BDNF (1:500; ab226843, Abcam) and NeuN (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used in this study: anti-SARS-CoV-2 spike glycoprotein rabbit polyclonal antibody (dilution 1:1000; #ab272420, Abcam); anti-SARS-CoV-2 nucleocapsid protein rabbit polyclonal antibody (dilution 1:1000 ...
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bioRxiv - Physiology 2019Quote: Uterine sections were blocked and permeabilized with 5% normal goat serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam) in 5% normal goat serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated for 2 hrs with a rabbit polyclonal pan-synuclein antibody (1:1000; ab53726; Abcam, Cambridge, MA). After washing in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Pathology 2021Quote: ... for 20 min before being incubated overnight with the primary anti-ACE-2 antibody (a polyclonal antibody raised in rabbits and diluted 1:2000, ab15348 Abcam) at 4°C in Tris-Buffered Saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 1x TBS and incubated for 2 hours at room temperature with appropriate secondary antibodies (goat anti-chicken FITC, 1:200, Abcam or goat anti-rat AF488 ...
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bioRxiv - Physiology 2020Quote: ... in 300 μl of TBS (with 2% goat serum and 0.2% Triton X-100) containing primary anti-desmin antibody (1:50; ab15200, Abcam, UK). After overnight incubation ...
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), CENP-E (mouse ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Cell Biology 2022Quote: ... Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam). All sections were mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were separated on a magnetic rack and washed once before being resuspended in 100 µl antibody buffer (wash buffer, 0.25% Digitonin and 2 mM EDTA) and 1 µl BRG1 antibody (Abcam, ab110641). Beads were incubated with the antibody over night with gentle shaking at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... groups of wildtype larvae (AB*) at 6 dpf were incubated for 2 hours in 30 mL EM with or without 1 mM cisplatin (Abcam) at 28°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated with the following primary antibodies diluted in blocking buffer at room temperature for 2 hours: anti-OCT4 (1:200, catalog no. ab181557; Abcam); anti-TFAP2C (1:100 ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocking was performed with 2% BSA in PBS for 30 min and cells were incubated with phalloidin-iFluor 594 reagent (1:1000; ab176757; Abcam) for 1 hour.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were blocked with goat serum for 2 hours at room temperature and incubated with RAD51(1:500 no. ab133534 Abcam) or yH2AX (1:200 no ...
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bioRxiv - Genomics 2023Quote: ... Liquid was removed on a magnet and beads were resuspended in 50uL ice-cold antibody buffer (wash buffer with 2 mM EDTA and 0.1% BSA) and 1 µL of antibody (anti-SON Abcam #ab121759) was added and incubated upright with gentle nutating overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: Equimolar mixture of histone octamer and LIN28B DNA (2 µM each) were mixed with 1 µM of Oct4 (abcam ab134876) and 3 µM of Sox2 (50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), BUBR1 (rabbit ...