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Citations for abcam :
1951 - 2000 of 2079 citations for 6 Chloro 3 fluoro 2 formylpyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with the SARS-CoV-2 Spike Antibody Mouse Mab (40592-MM117, SinoBiological, China) and HRP-conjugated goat anti-mouse IgG (ab6789, abcam, UK). Detection was carried out using an Ultra High Sensitivity ECL Kit (HY-K1005 ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 1% normal goat serum (NGS) for 1 hour and incubated overnight with primary recombinant anti-prolactin receptor antibody [EPR7184(2)] (abcam, ab170935) at 1:50 in 1% BSA ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation of the cleared lysates was performed overnight at 4C with 5μg of anti-VDR antibody or anti-RXRα monoclonal antibodies (Abcam – Table 2). The beaded complexes were washed several times with NP-40 lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubations were performed overnight at 4°C in PBS-T with 2% NGS plus one or more of the following antibodies: polyclonal rabbit anti-GFP (1:800, Abcam #ab290), monoclonal mouse anti-Abd-B (1:500 ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Western blot analysis was done as described (IHARA AND NISHIWAKI 2007) using anti-NID-1 (2 μg/ml) (KUBOTA et al. 2008) and anti-α-tubulin (DM1A, 1:1000; abcam), anti-GFP (3E6 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated in 50 μL of 2% goat serum in PBS containing mouse monoclonal anti-NR2A antibody (abcam #240884, 1:200) and rabbit monoclonal anti- Na+/K+-ATPase ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked at room temperature for 2 hours in TBTD supplemented with 10% donkey serum and incubated in anti- SOX2 (AbCam, #ab97959) and anti-SOX17 (R&D ...
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bioRxiv - Molecular Biology 2024Quote: ... H-2, sc-398394, 1:1000, Santa Cruz Biotechnology; anti-CPT1α: ab128568, 1:1000, Abcam; anti-ERRα: ab137489, 1:1000, Abcam). Imaging was done using KODAK films or the Amersham Imager 680 equipment.
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bioRxiv - Bioengineering 2023Quote: ... Blocking was conducted using 2% w/v BSA in 1×PBS for 1 h and incubated with anti-Ki67 antibody (Abcam ab16667) in a 1:200 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 2 hours at room temperature and incubated overnight at 4°C using the following concentrations: anti-H3K9me3 (1:300, Abcam ab8898), anti-H3K27me3 (1:300 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was then incubated in blocking buffer (5% skim milk in TBS/0.05% tween-20) followed by blotting with primary antibodies (anti-SARS-CoV-2 spike (Abcam ab275759, USA) 1:2,000 or anti-β-actin (Sigma A2228 ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked with 5% normal goat serum/2% BSA/0.1% Triton-X for 1 hour and incubated with mouse monoclonal PAX7 antibody (abcam, 1:200) and rabbit monoclonal SOX10 antibody (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed three times in PBS containing 0.01% Triton X-100 and incubated for 2 hours with AlexaFluor conjugated donkey anti-rabbit secondary antibody (Abcam ab150073 1:1000). After secondary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the same ACE2 IHC protocol was applied to other 2 primary antibodies anti-Human ACE2: monoclonal rabbit anti-Human ACE2 (cat. Ab108252, Abcam, Cambridge, UK) diluted 1:100 in PBS 1× supplemented with 5% goat serum ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were rotated for 2 hours at 4°C in 200 μL DigWash with 1:400 Lamin B1 antibody (Abcam, ab16048, rabbit), followed by a wash step with DigWash ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Genomics 2020Quote: ... Blocking with 1% BSA/PBS for 1h was followed by incubation with the following primary antibodies for 1-2 h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Microbiology 2020Quote: ... Each IP comprised of 100 μl of lysate and 2 μg of an antigen-specific antibody against p50 (ab7971, Abcam, Cambridge, UK), p65 (ab7970 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Western blots (2 µg per lane) were probed with primary antibodies specific for CD68 (ab213363, 1/500 in blocking buffer; Abcam, Cambridge, UK) or β-actin (4967S ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: ... The cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min at 37 °C with or without dye-labeled phalloidin (ab176753 or ab176759, Abcam, Cambridge, UK) for 1 h at 37 °C ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... Nuclei from one striatum (~10,000) were washed 2X and then resuspended in Digitonin Buffer with either anti-H3K4me3 (Antibody 1: Abcam, Ab8580 (Figure 2); Antibody 2 ...
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bioRxiv - Neuroscience 2021Quote: ... diluted in PBS plus 2% NBCS and 0.3% Triton X-100: mouse anti-Glutamate decarboxylase (65 kD isoform, GAD65; 1:1500; ab26113; Abcam, Cambridge, United Kingdom), rabbit anti-Calbindin (1:3000 ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Genomics 2022Quote: ... were washed twice with PBS/BSA and incubated with either 0.5 μl of anti-TBP antibody (gift from Dr. Joseph Reese, Pennsylvania State University) overnight or with 2 μl of anti-H3 (Abcam, cat # ab1791) for 3 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were blocked with 5 % (v/v) FBS in PBS for 30 min and incubated with a rabbit polyclonal anti-COX-2 primary antibody (ab15191, Abcam, Cambridge, UK) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was removed using a magnetic Eppendorf tube rack prior to adding 2 µg of anti-citrulline antibody (ACAb, Abcam, Cambridge, UK) diluted in 200 µL phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...