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Citations for abcam :
151 - 200 of 205 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Leukotriene B4 (LTB4) and Cysteinyl leukotrienes (LTC4, LTD4, and LTE4) were assessed in cell media using respective ELISA kits (Abcam). The results were normalised to the corresponding CellTiter-Blue viability results or cell count.
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bioRxiv - Biochemistry 2022Quote: SISA strains KS8266 and KS8311 (Mustavchiev et al., 2016) were maintained on Ye5S media containing 5μM 3-BrB-PP1(Abcam, ab143756) to prevent activation of sty1 and retention of Wis1DD was confirmed by sequencing of cells used for experiments ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBST three times and then mounted onto MAS-GP microscope slides (Matsunami) and coverslipped with Fluoroshield Mounting Media (Abcam). Images were collected on a confocal microscope (Zeiss LSM 710 ...
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bioRxiv - Molecular Biology 2023Quote: ... For GeneSwitch experiments flies were placed on media supplemented with either vehicle (EtOH) or varying concentrations of RU486 (Abcam, ab120356). In experiments where the Gal4/Gal80ts system was utilised ...
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bioRxiv - Cancer Biology 2023Quote: ... PSA levels were quantified both in the conditioned media and in the mouse sera using the PSA ELISA kit (Abcam), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... and plated into a Matrigel-coated 96-well plate with mTESR media containing ROCK inhibitor Y-27632 (10 uM, Abcam) at 30,000 cells per well one day before transfection ...
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bioRxiv - Genomics 2023Quote: Standard media was added to mESC E14 or K562 cells with a final concentration of 10 uM BrdU (abcam ab142567). Cells were incubated in BrdU-containing media for between five minutes and 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... After an additional 24 hours the media was either filtered onto SHSY5Y receiver cells or 10ml was added to 2.5ml PEG-IT reagent (Abcam, ab1025238) and stored at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... cultures were treated with 7-Chlorokynurenic acid (7-CKA) (final concentration 50 μM; 1 μL stock solution in DMSO in 1 mL media; ab120024; Abcam) and D-AP5 (final concentration 100 µM ...
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bioRxiv - Genomics 2024Quote: Exponentially growing NIH3T3 cells were stimulated by adding one volume of fresh media containing 10 ng/mL TNFα (Abcam, ab9642), for a final concentration of 5ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed for 5 mins in PBS and mounted with mounting media containing DAPI (catalog number ab104139, Abcam) before imaging.
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bioRxiv - Cell Biology 2020Quote: ... cell culture medium was aspirated and iPSCs were incubated in 2 mM EGTA (Carl Roth) solution in PBS for 20 mins followed by incubation in culture medium containing Y-27632 ROCK inhibitor (Abcam). Control samples received knockout DMEM (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 0.25 x 106 cells were seeded into 10 mL cell culture dishes then treated with 5 ng/mL TGFbeta (Abcam) on the next day ...
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bioRxiv - Cell Biology 2022Quote: ... cultures were incubated for 1 h at room temperature with primary antibodies as follows: rabbit anti-VE-cadherin (ab33168, Abcam), mouse anti-paxillin (MA5-13356 ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation of HeLa cells transfected with the ZC3H11A-Flag expressing plasmids (5μg plasmid/100mm tissue culture plate) was done using Cell Fractionation Kit (Abcam, ab109719) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Free glycerol was measured in the infranatant fraction of the isolated adipocyte culture using a colorimetric assay kit (ab65337, Abcam) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: The amount of α-synuclein in the culture medium was measured using a mouse α-synuclein ELISA kit (Abcam ab282865) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Cancer Biology 2023Quote: ... Following 24 hours to allow for TEV uptake DCs culture on glass slides was stained with Cytopainter Red (cat# ab219942, Abcam) for 30 minutes according to the manufactures instructions and fixed with 4% formaldehyde for 30 minutes and washed 5 times with PBS ...
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bioRxiv - Cancer Biology 2023Quote: The acetyl-CoA content of the cell culture medium was determined with the Acetyl-CoA Assay Kit (Reference: ab87546, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... CMs cultured on Matrigel-coated cell culture plates were harvested and stained for Anti-Cardiac Troponin T (clone 1C11) Antibody (Abcam) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: Enzymatic activity of secreted LOX was measured in 10 μl cell culture supernatants using the fluorometric Lysyl Oxidase Activity Assay Kit (Abcam). LOX activity kinetics was evaluated by measuring fluorescence (Ex/Em = 535/587 nm ...
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bioRxiv - Molecular Biology 2024Quote: Mycoplasma PCR test was performed on culture supernatants (at least 48h without medium change and confluence at least 80%) using Kit Mycoplasma PCR Detection Kit (Abcam) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: Levels of glucose and glutamine were determined via analysis of spent culture medium (n=4/group) using a Glucose Assay Kit and a Glutamine Assay Kit (Abcam), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... NOK805 and D6 cells were seeded into 6-well plates at a density of 2,5 × 105 cells per well in antibiotic free media with 10 μg/ml Y-27632 (Abcam, Cambridge, UK) and left to adhere at 37 °C and 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: Cell media testosterone levels were determined using a commercially available testosterone ELISA assay according to the manufacturer’s protocol (#ab108666, Abcam, Cambridge, UK).
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bioRxiv - Physiology 2022Quote: ... transwell filters were removed from plastic retaining rings and mounted onto slides with Fluoroshield + DAPI mounting media (Abcam; Cambridge, MA USA). For co-staining with direct labeling of primary antibodies ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were added to incubate for an hour in the dark at room temperature before being washed again with PBS and PBST and mounted on positively charged slides with DAPI Fluoroshield mounting media (Abcam ab104139). Antibodies and dilutions used for immunofluorescent staining can be found in Table S2.
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bioRxiv - Neuroscience 2024Quote: Primary hippocampal neurons treated with Tris and PFFs at various time points were incubated in growth media with 1 μM Fura-2 AM (Abcam, UK) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: C3/C3b ELISA to evaluate the concentration of C3/C3b in cell culture supernatants was performed according to the manufacturer’ ss instructions (Abcam, UK). Samples and standard controls were loaded in 96 well-plates coated with specific C3b antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... and virus replication was quantitated by measuring the p24 antigen released in culture supernatants at different time points using HIV-1 p24 SimpleStep ELISA kit® (Abcam). Serial dilutions of HIV-1 p24 protein (strain 92418 ...
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bioRxiv - Bioengineering 2020Quote: ... we also confirmed cardiac differentiation on dECM-fibrin samples and tissue culture plates using an antibody specific to cardiac troponin T (Abcam, ab8295). This data is provided in supplementary figure S1.
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bioRxiv - Developmental Biology 2022Quote: ... protein was extracted from the 8 cultures using the lysis buffer from the GFP Fluorescent ELISA Kit (ab229403) (Abcam, Cambridge, UK) that was supplemented with Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: One hundred µl of the cell culture was transferred into a 96-well microplate provided by the In-Cell ELISA kit (Abcam, ab111541), and treated following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were then incubated at 4C overnight with an and a rabbit monoclonal antibody against either Golgin-97 (Abcam, #ab84340) or Calnexin (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... was used for AnkB localization in cortical neurons in culture by immunofluorescence staining with mouse monoclonal anti-AnkB antibody 33-3700 and AF-594-conjugated secondary antibodies (Abcam, #ab245992). STED images were acquired using a Leica SP8 STED confocal microscope with Lightning deconvolution in the UNC Neuroscience Research Center.
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bioRxiv - Molecular Biology 2023Quote: ... Cultures were then washed 5x 5 minutes each and incubated with Alexa594-conjugated Goat α-Mouse IgG H&L (Abcam, #ab150116) diluted 1:500 in wash buffer for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: NRVMs were transduced with shCtrl or shAtg7 for 48 hours and the supernatants of the cultures were collected for cytokine array analysis using a rat cytokine array kit (Abcam, # ab133992) according the protocol provided by the manufacturer.
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bioRxiv - Cell Biology 2024Quote: The concentrations of S100A8/S100A9 in the culture medium were quantitatively determined utilizing a Human Calprotectin ELISA Kit (S100A8/S100A9) (Abcam, ab267628), adhering strictly to the manufacturer’s prescribed protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The cell pellet was resuspended into an osteoclast differentiation media constituting of RPMI 1640 supplemented with 40 ngml-1 phorbol 12-myristate 13-acetate (PMA, Abcam, Cambridge, UK), 10 ngml-1 receptor activator of nuclear factor-kappa ß ligand (RANKL ...
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bioRxiv - Cell Biology 2023Quote: ... MDM treated with or without actin remodelling inhibitors as above were washed and incubated with 50 μL serum-free media and 50 μL of MTT reagent (Abcam MTT assay) per well ...
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bioRxiv - Bioengineering 2022Quote: ... Phenol-red free media surrounding scaffolds for 14 days of hMSC cell culture on scaffolds was pooled and 80 μL of this sample was used with an Alkaline Phosphatase Assay Kit (Abcam, United Kingdom) along with phenol-red free media as a background control (n=6) ...
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bioRxiv - Neuroscience 2020Quote: The glutamate concentration in the conditioned medium of primary microglial cultures was assayed with the glutamate colorimetric assay kit from Abcam (no. 83389), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Secreted RANKL and OPG were quantified in cell supernatants from day 7 of MC-MSC co-cultures with RANKL (ab213841, Abcam, Cambridge, UK) and OPG (EHTNFRSF11B ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocyte cell culture samples were probed for glutamic acid decarboxylase (GAD) 65-67 using rabbit anti-GAD 65-67 (Abcam, catalog #183999) with the corresponding secondary HRP-conjugated antibody and re-probed for β-actin (as described above) ...
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).