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Citations for abcam :
151 - 191 of 191 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared products were collected and incubated in fresh Protein A agarose beads and anti-huntingtin EPR5526 (Abcam) for 2 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose® 4 Fast Flow beads (Cytiva) bound to 1 µg of anti-TRIB3 (abcam, ab75846) were incubated with 500 µg of cell lysate for 2 hours at 4°C with rotation ...
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bioRxiv - Immunology 2024Quote: ... plasma sample at 10% in complete media was incubated with Protein A/G sepharose beads (Abcam, Boston, MA) for 1 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Washed beads were resuspended in 400µl lysis buffer and 5µl anti-Piwi (Hannon Lab) or rabbit IgG antibodies (Abcam, ab37415) added ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting nuclear extract was incubated with Dynal protein G beads which were preincubated with 5μg of H3K27me3 or H3 antibody (ab6002 and ab1791, Abcam) respectively at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... male) transfected with miR-145 or control mimics for 24h using 90 μl Sepharose G beads (Abcam, Cambridge, UK) pre-coated with 6ug of mouse anti-human Ago2 (clone2E12-1C9 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then removed buffer and resuspended cells and beads in BD staining buffer with Annexin V-Cy5 (Abcam Cat# ab14150) incubated for 10min in dark at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were resuspended in 1 mL ChIP dilution buffer with 8 μg of anti-HA antibody (Abcam ab9110) and incubated on a rotator at 4°C for 6 h.
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bioRxiv - Genomics 2024Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... beads were resuspended in 50 μl of WaB-Dig containing LaminB1 antibody (Abcam, 1:100 of 1 mg/mL). The sample was incubated at 4 °C on nutator for 2 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... The beads were resuspended in 1 mL ChIP dilution buffer with 8 μg of anti-HA antibody (Abcam ab9110) and incubated on a rotator at 4°C for 6 h.
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cancer Biology 2022Quote: The bead-lysate mixtures were centrifuged at 1500 rcf for 1 min at 4°C and each lysate was transferred to a tube containing ∼37.5 µL pre-washed beads and 15 µL of HLA-II antibody mix (9 µL TAL-1B5 (Abcam, ab20181), 3 µL EPR11226 (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: EVs were separated and concentrated from 1 mL of plasma as described above and then analyzed by flow cytometry using CD9 exosome capture beads (ab239685, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Nuclear extracts from 2L×L105 UCB monocytes purified using an anti-CD14 antibody coupled to magnetic beads per the manufacturer’s recommendation (MACS, Miltenyi Biotech, San Jose, CA) were isolated per the manufacturer’s instructions (Abcam, Cambridge UK) and quantified using a micro-BCA assay protein assay kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A (if the antibody host was rabbit) or Protein G (if the antibody host was mouse) agarose beads (Abcam) were washed three times in aCSF and centrifuged at 400 g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The precleared supernatant was divided into two Eppendorf tubes and beads were either cross-linked to the bait antibody (Grin2b-rabbit #06-600 Millipore,Ppp1ca-Abcam Rabbit #ab16446 ...
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bioRxiv - Genomics 2023Quote: ... Beads were then resuspended in 1 mL BSA solution and 5 μL of α-5-hmU antibody (Abcam Cat # ab19735) were added ...
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bioRxiv - Neuroscience 2024Quote: ... 5mg per sample of lysate was added to 50ul Protein G beads (Cytiva 28951379) pre-bound with 10ul anti-PCB (Abcam 110314 ...
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bioRxiv - Pathology 2024Quote: ... Each pre-cleared chromatin sample was then incubated with 35μl of protein A/G agarose beads pre-bound with 10μl of ChIP-grade rabbit anti-ESR1 antibody (Abcam, #ab32063) at 4℃ overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... were prepared in order to directly crosslink antibodies to the beads (anti-GAF, gift from G. Cavalli, or anti-H4K8ac, abcam 15823), avoiding cross reaction with the H3S10ph antibody ...
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bioRxiv - Microbiology 2020Quote: ... or IgG were used to immunoprecipitate the chromatin fragments in the supernatant by overnight incubation using protein A sepharose beads (Abcam, UK) at 4 °C.The beads were washed twice with wash buffer ...
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bioRxiv - Physiology 2022Quote: ... 50-100 μg of protein were used for the pull down of FLAG-tagged Kcnq4 proteins with monoclonal anti-FLAG M2 antibody produced in mouse (mouse monoclonal Anti-FLAG M2 antibody, F3165) and Protein G Sepharose beads (Abcam, UK) at 4 °C overnight ...
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bioRxiv - Genomics 2022Quote: ... The ChIP assay was performed with Dynabead Protein G beads and histone antibodies (H3K4me3: Millipore #07-473, H3K4me1: Millipore #07-436, H3K27me3: Millipore #07-449, H4K27ac: abcam ab4729). After binding to antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were removed and nuclear extracts were incubated overnight at 4°C with either anti-lamin A/C antibody (Abcam, #ab108595) or non-immune IgG ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation was accomplished using plate based-beads in the EpiQuik Kit along with HNF4α antibodies (Novus NB100-1783 and Abcam ab181604) to validate enrichment at QPRT ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were enriched by ConA Beads and resuspended by 50 µL primary antibody buffer of anti-pMad (1:50, Abcam, ab52903), and incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 200,000 SYAT cells were bound on Concanavalin A beads prior to incubation overnight with 1:50 anti-GFP rabbit antibody (Abcam #ab290). Next ...
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bioRxiv - Developmental Biology 2024Quote: ... Bead-bound cells were incubated with a SOX2 (CST, clone D9B8N, 23064, 1:100) or H3K9me3 antibody (abcam ab8898, 1:100) at 4C overnight ...
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bioRxiv - Immunology 2024Quote: ... The exosomes/beads complexes were incubated with primary anti-human antibodies against CD63 (anti-CD63 antibody [EPR5702], Abcam, Cambridge, MA, USA) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... the surface of the coverslip and the platform beads are functionalized by flowing anti-GFP antibody (product #ab1218, Abcam, Cambridge, United Kingdom) solution in AB buffer (25 mM imidazole at pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Developmental Biology 2024Quote: ... Beads were resuspended in 150 µl of CUT&Tag wash buffer with 1.5 µl of secondary antibody (Abcam, 46540; Antibodies Online, ABIN101961), and incubated for 1 hour at 4 °C on a nutator.
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed into a LoBind tube carefully so as not to remove any beads and 2 µg of anti-GFP antibody (Abcam ab290, anti-rabbit) were added to the sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... Pre-washed beads were incubated with antibodies (Normal Rabbit IgG, Millipore Sigma, Cat. #12-370; Normal mouse IgG, Cell Signaling Technology, Cat. #68860L; MAFG, Abcam, Cat ...
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bioRxiv - Genomics 2023Quote: ... were blocked and pH-calibrated as per the manufacturer’s protocol and 5 μl bed volume of beads were loaded with 2 μg of ChIP antibody (H3K27ac; Abcam-AB4729, H3K115ac; PTM BIO-PTM-170). Beads were added to the solubilized chromatin ...