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Citations for abcam :
151 - 200 of 3761 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or 2 µM Pitstop-2 (Abcam), hypotonic medium (80% of medium was replaced with water ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein expression was assayed by Western blotting cell supernatant using anti-6xHis-HRP antibody (Abcam). Proteins from transient transfection were purified as follows ...
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bioRxiv - Cancer Biology 2020Quote: Histone proteins were extracted from treated or untreated cells using a histone extraction kit (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-human antibodies were used for the following proteins: CDK12 (Cell Signaling 11973S, Abcam ab246887), β-Actin (Santa Cruz sc47778) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated for 2 hrs with anti-αSYN ab52168 (Abcam, 1:100 dilution) in blocking buffer ...
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bioRxiv - Physiology 2021Quote: Differentiated Caco-2 cells in transwells were stained with H&E stain (Abcam, ab245880) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... live single-cell preparations were stained with 20μM 2’,7’ – dichlorofluorescin diacetate (DCFDA, Abcam) for 5 minutes in HBSS ...
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bioRxiv - Neuroscience 2023Quote: ... LRRK2 kinase activity was inhibited pre-treating cells with 0.5μM MLi-2 (ab254528, Abcam) for 90 minutes.
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bioRxiv - Cancer Biology 2024Quote: ... p-LATS1/2 (T1079/T1041) (Cell Signaling, D57D3, Abcam, ab305029, 1:1,000 – 1:3,000), cJUN (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTS) cell proliferation assay (Abcam, ab197010). Gadodiamide (GdDTPA-BMA ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with primary antibody for 2 h Anti-TFEB Antibody (Abcam, ab2636). After washing 3 times in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Pathology 2019Quote: ... Soluble proteins (20 μg) were used for Western blotting with primary antibodies against AKT1/2/3 (rabbit monoclonal antibody; EPR16798 Abcam; 1:10,000), mTOR (rabbit monoclonal antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... Proteins were stained using InstantBlue Coomassie protein stain (Abcam). Molecular weights were manually estimated according to the Novex Sharp protein marker ...
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bioRxiv - Physiology 2020Quote: ... Anti-Influenza A Virus Nucleoprotein (NP; NR-43899, BEI Resources, Manassas, VA) or Anti-Thrombospondin 1 (ab85762, abcam, Cambridge, UK), with secondary antibody AlexaFluor 594 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The culture supernatants containing pseudotyped viruses particles were either used immediately or flash frozen in aliquots and stored at 80°C until use after being concentrated using a PEG virus precipitation kit (Abcam). Incorporation of the SARS-CoV-2 S protein into the SARS-CoV-2pp was confirmed using Western Blot analysis with anti-SARS-CoV-2 S1 chimeric monoclonal antibody with combined constant domains of the human IgG1 molecule and mouse variable regions (40150-D001 Sinobiological ...
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bioRxiv - Systems Biology 2023Quote: ... These were followed by subsequent staining with 50 μl of primary antibody (Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193, Abcam), 1:500 dilution ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and stored at -80°C or further concentrated using a polyethylene glycol (PEG) virus precipitation kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T cells were treated with either the fluorescent glucose analogue 2- (N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, 50μM, 1h, Abcam) or kynurenine (800μM in HBSS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Interleukin 2 (IL-2, Abcam, Cambridge, UK) was used at a final concentration of 1 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (Abcam Catalog No. ab146200) was dissolved in DMSO to yield a 20 mM stock solution ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were tested for expression of the fusion protein by Western blot analysis for ApoA1 (Abcam), ApoM (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: Citrullinated proteins were immunoprecipitated from whole cell extracts with an anti-pan-citrulline antibody (Abcam, ab6464). Samples were concentrated on Amicon Ultra 0.5 mL columns (cutoff ...
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bioRxiv - Neuroscience 2022Quote: We have also tested antibodies for a number of other cell adhesion proteins (ITGB1 (abcam, ab24693), ITGA6 (abcam ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were used for detection of cell-surface proteins: calreticulin (FMC 75 mAb; Abcam); human CD4 (clone SK3 ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were first stained with following antibodies: rabbit-anti-human pCD3ς (Abcam, EP776(2)Y) ...
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bioRxiv - Genetics 2020Quote: ... in HUDEP-2 cells expressing an HA-tagged ZNF410 construct or anti-ZNF410 (Abcam #ab174204) to detect endogenously expressed ZNF410 in HUDEP-2 and primary human CD34+ cells as well as endogenously expressed Zfp410 in MEL cells ...
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bioRxiv - Physiology 2021Quote: ... HEK-293 cells were cultured and treated with 2 µg/mL of tunicamycin (ab120296, Abcam) for 6 hours ...
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bioRxiv - Cell Biology 2020Quote: ... The transfected cells were stained with 1:100 dilution of anti-mouse BST-2 (Abcam) or isotype control antibody (R&D system ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell nuclei were stained with 4’,6-diamidino-2-phenylindole staining solution (ab104139, Abcam) at an ambient temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 20□μM of 2’,7’– dichlorofluorescin diacetate (DCFDA) (ab113851) (Abcam, USA) for 30□min at 37□°C and analysed on an Accuri C6 flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed with acetone:methanol (40:60) solution and immunostaining was performed using an anti-Vaccinia Virus antibody (1:4000 dilution, #ab35219 Abcam, Berlin, Germany), which was detected with a peroxidase-conjugated anti-rabbit secondary antibody (1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... staining was performed overnight at +4⁰C with antibodies to the following targets: influenza A virus (IAV) polyclonal antibody (1:400, goat, Abcam, ab20841), NP (IAV ...
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bioRxiv - Cell Biology 2023Quote: ... This viral supernatant was then concentrated by centrifugation at 3200 x g for 30 minutes at 4°C before being resuspended in 100 µl virus suspension solution (Abcam, ab102538), aliquoted and stored at -70°C ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Polyclonal antibodies against extracellular signal-regulated kinase 1/2 (ERK1/2; ab196883) and monoclonal antibodies against phospho-ERK1/2 (p-ERK1/2; ab201015) were purchased from Abcam. Protein expression was normalized to GAPDH (sc-2577 ...
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bioRxiv - Immunology 2021Quote: ... 2-N-nitrobenz-2-oxa-1,3diazol-4-amino]-2 deoxy-D-glucose (2NBDG) (Abcam). CD4+ cells were cultured for 24h in anti-CD3 coated plates (0.5μg/ml ...
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bioRxiv - Systems Biology 2019Quote: ... Isolation and protein extraction of mitochondria was performed by Mitochondria Isolation Kit for Cultured Cells (ab110170. abcam). Proteins were separated by Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... HIF-1α protein was extracted from cell pellets and confirmed using Human Simple Step ELISA kit (Abcam) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... cells were treated with 4 μg/ml of Recombinant Mouse α-Syn Protein Aggregate (Active) (Abcam, ab246002) for periods of 24 ...
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bioRxiv - Physiology 2020Quote: ... Protein concentrations were measured using BCA Protein Assay Reagent (Abcam, USA). Proteins were separated on SDS-PAGE and transferred to the polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with 1:500 anti-SARS-CoV-2 Spike glycoprotein antibody (Abcam, Catalog#ab272504) diluted in blocking buffer for overnight at 4 degree ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... cells were subjected to ChIP assay with primary antibody against ESR1 (2 μl/IP, Abcam #ab32063) or normal rabbit IgG (1 μg/IP ...
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bioRxiv - Microbiology 2019Quote: The detection of specific anti-RSV IgG antibodies in biological fluids was performed using Human Anti-Respiratory syncytial virus IgG ELISA Kit (ab108765, abcam®, Sweden), according to manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Protein quantification was performed with the BCA protein assay kit (ab207003, Abcam). Proteins were separated by SDS-PAGE and transferred to PVDF membranes ...