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Citations for abcam :
151 - 200 of 2476 citations for 7 Trifluoromethyl quinazoline 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitation was performed overnight at 4°C with rotation using 1-2 × 106 cell equivalents per immunoprecipitation using antibodies (5 µg) against H3K4me1 (Abcam), H3K27ac (Abcam) ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... blocked spheroids were firstly incubated for 2 days at 4 °C with α-Ki67 (Abcam, stock concentration 0.031 mg/mL) or α-cleaved caspase3 (Cell Signalling Technology ...
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bioRxiv - Evolutionary Biology 2021Quote: ... pCS2-lg-HAtag-WT and pCS2-lg-HAtag-MUT (2 μg) (Supplementary Fig. 4) were then transfected into the HEK293T cell line (ab255449, Abcam) separately ...
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bioRxiv - Genetics 2020Quote: ... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Neuroscience 2023Quote: D-AP5 (D-(−)-2-Amino-5-phosphonopentanoic acid) and SR 95531 (2-(3-Carboxypropyl)-3-amino-6-(4 methoxyphenyl) pyridazinium bromide) were purchased from Abcam, UK ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with TBST containing 5% skim milk or 5% BSA for 2 hours and incubated overnight at 4°C with the following antibodies: anti-MIF (1:200, ab65869, Abcam), anti-iNOS (1:500 ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times in PBS before the addition of 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, Cambridge, UK) diluted 1:10,000 in H2O ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Immunology 2024Quote: ... and were rinsed with PBS for three times before mounting the coverslips to slides in glycerol mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (ab188804, Abcam). The mounted samples were observed on a Zeiss LSM 880 inverted laser scanning confocal microscope ...
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bioRxiv - Immunology 2022Quote: ... 4-Hydroxynonenal (4-HNE, 1:200; Abcam, # ab48506), Alexa Fluor 555 donkey anti-rabbit (1:300 ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... and treated with blocking solution (TBS containing 5% normal goat serum and 0.2% Triton X-100) for 2 hr and incubated overnight at 4°C with chicken anti-GFP monoclonal antibody (1:500; abcam; ab13970). After washing three times with TBST (TBS containing 0.2% Triton X-100) ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The secondary antibody was then washed with 1X PBS three times and the nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole)-containing mountant (Abcam Ab104139). Samples were imaged with a motorized fluorescence microscope (Olympus IX83 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Immunology 2024Quote: ... chromatin from approximately 106 cells was incubated overnight at 4°C with 2 μg of anti-ERRα or anti-PGC-1α (Abcam, USA) ChIP-grade antibody and 20 μL of A/G magnetic beads ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were rotated for 2 hours at 4°C in 200 μL DigWash with 1:400 Lamin B1 antibody (Abcam, ab16048, rabbit), followed by a wash step with DigWash ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... with fluorochrome for 1 hour in the dark and the samples were then mounted with Fluoroshield mounting medium with DAPI (4’,6-diamidino-2-phenylindole, Abcam, Cambridge, MA). Slides were imaged using the Keyence BZX-700 Fluorescent Microscope (UCSD Microscopy Core).
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4° C with the primary monoclonal mouse antibody MOAB-2 against beta amyloid (1:500; Abcam Cat# ab126649) (Youmans et al. ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... The samples were incubated at 4℃ for 48 hours with mouse anti-CaMKIIα antibodies (ab22609, abcam, 1:250 dilution in 2% NGS). After treating the primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... CCK-4 (Abcam), CRF (Anaspec) ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by goat anti-rabbit IgG (HRP, Abcam Lot#GR3231028-7, 1:20,000). The bottom half was probed with MAP-1B (LC1 ...
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bioRxiv - Genetics 2020Quote: ... or GATA1 (∼7.2 µg ab11852, lots: GR208255-9, GR290167-7, GR239235-8; AbCam) overnight ...
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bioRxiv - Developmental Biology 2019Quote: ... after 7 days of culture for Stra8 (Abcam Inc., Cambridge, MA, USA, ab49602), a marker for differentiated spermatozoa ...
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bioRxiv - Neuroscience 2020Quote: ... Non-viable cells were excluded by staining with 7-AAD Staining Solution (Abcam) for five minutes and pHrodo fluorescent signal was analyzed from 20000 live cells by flow cytometry ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used: anti-Cytokeratin 7 (1/8,000, ab181598, abcam), and - Monocarboxylic acid transporter 1 (1/1,800 (0.5 μg/ml) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sca-1/Ly6A/E (E13 161-7, Abcam, Cat#ab51317, 1:200 IF), YAP1 (63.7 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µM Rhod-2 AM (Abcam) were loaded for 30 min at 37 °C in buffer P ...