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Citations for abcam :
1901 - 1950 of 2150 citations for Recombinant Human YKT6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were probed in Can Get Signal Solution1 (Toyobo) using following primary antibodies: anti-Gpx4 (1:10,000, Abcam, ab125066), anti-β-actin (1:20,000 ...
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bioRxiv - Genomics 2024Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were washed once with distilled water and stained with InstantBlue® Coomassie Protein Stain (Abcam, Cambridge, UK). The gels were viewed using a ChemiDOC XRS molecular imager (Biorad ...
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bioRxiv - Immunology 2023Quote: ... Obtained protein fraction has been subjected to Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Microbiology 2023Quote: ... or mouse antibodies recognizing cellular proteins (anti-EEA1, BD Biosciences, 610457, 1:100; anti-VPS35, Abcam, ab57632, 1:200); rabbit antibodies recognizing cellular proteins (anti-TGN46 ...
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bioRxiv - Genomics 2023Quote: ... 500 mM NaCl and 0.2% Tween 20) and precleared with 100 μL 50% protein G Sepharose bead (Abcam, ab193259) slurry for 20 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was transferred overnight to 0.22µ PVDF membranes and probed using either anti-HA (Abcam Cat# ab9110, RRID:AB_307019) or anti-Myc (Abcam Cat# ab32 ...
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bioRxiv - Biochemistry 2022Quote: ... and the quality of the purification was assessed via SDS-PAGE followed by InstantBlue Coomassie protein stain (abcam: ab119211) staining and immunoblotting ...
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bioRxiv - Biochemistry 2023Quote: ... Then gels were either stained with Coomassie-blue (15 µg of protein per lane) (Instant blue, abcam, Cambridge, UK) or transferred to nitrocellulose membranes (0.25 µg or 1 µg of protein per lane for the monomeric transporters and heterodimer respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight in primary antibodies against green- fluorescent protein (GFP, 1:1000 in antibody diluent, Abcam, ab13970), calcitonin gene-related peptide (CGRP ...
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bioRxiv - Molecular Biology 2023Quote: ... In each capillary 0.2mg/mL protein was loaded for analysis with antibodies to dystrophin (Abcam # ab15277, dilution 1:15) as in 26 ...
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bioRxiv - Genomics 2024Quote: ... and ∼10 µg total protein was used to measure TET activity using the TET Hydroxylase Activity Quantification Kit (Abcam) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: Protein was visualized by SDS-PAGE analysis using 4-12% bis-tris mPAGE gels and stained using InstantBlue (Abcam), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450) and a 1:5000 dilution of donkey anti-goat IRDye800CW (Licor 926-32214) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Biophysics 2020Quote: ... All sections were permeabilized with 0.2% Triton X-100 in PBS for 15 minutes and non-specific protein binding was then blocked by incubation in a commercial blocking agent (ab156024, Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Antibodies against phospho-Heat Shock Protein 27 (S82) and phospho-c-Jun (S73) were obtained from Abcam (Cambridge, United Kingdom), while phospho-JNK (T183/Y185 ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted proteins were analyzed on a 6% SDS-PAGE gel followed by a western blot using monoclonal anti-GST (Abcam) or anti-MBP (New England Biolabs ...
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bioRxiv - Genomics 2021Quote: ... one piece corresponding to the higher protein ladder size for the incubation of RAD21 and CTCF primary antibodies (RAD21: anti-RAD21 antibody, rabbit polyclonal, Abcam, ab154769 ...
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bioRxiv - Microbiology 2022Quote: ... α-surfactant protein D (primary: polyclonal, Abcam catalog no. ab203309; secondary: Donkey anti-Rabbit IgG, AlexaFluor 405, Abcam catalog no. ab175651), α-mannose binding lectin A (primary ...
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bioRxiv - Molecular Biology 2021Quote: Expression of GLIS1-βGAL fusion protein was examined by staining tissue sections with chicken anti-βGAL (1:500, ab9361, Abcam) and Alexa Fluor@ 488 donkey anti-chicken IgG (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Physiology 2019Quote: ... rabbit acetylated SOD2 (K122), rabbit SOD2, and rabbit OXPHOS cocktail (to analyze NDUFB8, SDHB and UQCR2 protein content) from Abcam. Fluorescent anti-mouse or anti-rabbit secondary antibodies (red ...
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bioRxiv - Biochemistry 2019Quote: ... and equal amounts of proteins were used for each condition that was analyzed by immunoblotting with following antibodies: UCHL1 (ab27053; Abcam), Tubulin (2148 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes containing proteins were blocked in 5% BSA and incubated with rabbit anti-HMGB1 polyclonal antibody (1:1,000, Abcam, U.S.A), followed by incubation with horseradish peroxidase-linked secondary antibodies (1:5,000 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Bioengineering 2019Quote: ... cells were incubated with primary antibodies against microtubule-associated protein 2 (MAP2) (rabbit polyclonal, 1:100, ab32454; Abcam, Cambridge, UK) or Beta III Tubulin (β3TUB ...
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bioRxiv - Plant Biology 2019Quote: ... the corresponding protein bands were probed with a goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5000 dilution, Abcam, ab205718) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... for 6 h and then fixed with methanol for immunostaining with an antibody against nuclear pore complex proteins (clone mAb414, Abcam) following the regular immunofluorescence protocol described below ...
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bioRxiv - Cancer Biology 2019Quote: ... 30 μg of protein lysate from each fraction was analyzed by western blotting using the anti-HER4 antibody E200 (Abcam) to determine HER4/4ICD subcellular localization ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Cell Biology 2021Quote: ... ovalbumin and CD4-Fc fusion proteins were labelled with allophycocyanin (APC) with the Lightning-Link Labelling Kit (Abcam, Cambridge, UK).
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-mouse postsynaptic density protein 95 (PSD-95, 1:1000 for cells, 1:200 for tissues, Abcam, catalog ab99009), rabbit anti-mouse vesicular gamma aminobutyric acid (GABA ...
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bioRxiv - Microbiology 2021Quote: ... PBS for 45 minutes and further incubated in blocking solution containing anti-SARS-CoV-2 spike protein subunit 2 (Abcam) 1:1000 dilution for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: Iron saturation of transferrin was measured on 6% TBE-urea gels as previously described.32 Gels were stained with InstantBlue Coomassie protein stain (abcam).
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Immunology 2021Quote: ... the HIV Env 1086.c K160N gp120 protein was conjugated to biotin using the Fast Type A Biotin Conjugation kit (Abcam) and then captured on avidin-labeled fluorescent beads (NeutrAvidin™ ...
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Biochemistry 2022Quote: ... The supernatants were incubated with an anti-TIN1 antibody followed by protein-A/G-agarose beads (Abmart) or with anti-GFP Sepharose beads (Abcam) at 4 °C for 6 h ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Physiology 2022Quote: ... blocked in 5% goat serum for 1 hour and stained using myelin protein zero (MPZ) (1 in 50 in 5% goat serum in PBS containing 1% triton) (EPR20383, Abcam) overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The molecular masses of the protein bands were assessed by comparing with the mass of the bands in the lane containing the standards (Abcam). The experiment was performed in triplicate and the bands corresponding to the heterodimer and heterotetramer were visualized in all the three experiments.
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bioRxiv - Biochemistry 2022Quote: ... Separated proteins were transferred to polyvinylidene difluoride (PVDF) membranes and immunoblotted with antibodies specific for SF3B1 (Abcam, catalog no. ab170854), SF1 (Bethyl Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919; goat anti-EHD2, 1:200, Abcam 23935 ...
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bioRxiv - Biophysics 2022Quote: ... 1:1000 dilution in blocking buffer) plus antibodies against respective caveolin accessory proteins as indicated in figures (rabbit anti-cavin1, 1:200, Abcam 76919 ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...