Labshake search
Citations for abcam :
1901 - 1943 of 1943 citations for QuantiChrom Aldehyde Dehydrogenase Inhibitor Screening Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Bioengineering 2024Quote: ... were used to quantify the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (Abcam; Cambridge, UK) as an indicator of oxidative stress levels and redox imbalance.33
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Molecular Biology 2023Quote: The main pro-inflammatory and anti-inflammatory cytokines in blood and heart were measured by corresponding ELIZA kit: tumor necrosis factor-ɑ (TNF-ɑ, Abcam, ab100747), interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Neuroscience 2023Quote: ... using V-PLEX Pro-inflammatory Panel 1 (Meso Scale Discovery, catalogue no. K15048D1) and mouse apolipoprotein E ELISA kit (abcam, catalogue no. ab215086) according to the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2023Quote: Serum vitamin D (25(OH)D) levels of mouse serum samples were assessed by commercially available ELISA kits (Eagle Biosciences, Inc. Amherst NH, or Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Three 5 µm sections taken at 150 µm intervals were used for analysis from each animal and were stained with hematoxylin and eosin using the reagents provided in Oil Red O Stain Kit (Abcam, Waltham, MA, USA) for hematoxylin and eosin purchased from StatLab (StatLab ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and the quantification of intracellular and extracellular total cholesterol and its fractions was measured using the Cholesterol Assay Kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: LB samples that were autoclaved or not autoclaved were subjected to quantification of NADH/NAD+ using an NAD+/NADH Colorimetric Assay Kit (ab65348, Abcam, Cambridge, United Kingdom). Autoclaved samples were measured before and after either chronoamperometry or cycle voltammetry treatment ...
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bioRxiv - Immunology 2022Quote: ... TSLP and pCol1 in cell culture supernatants were determined using ELISA kits (R&D System for Areg, IL-33 and TSLP, and Abcam for pCol1). Unless stated otherwise ...
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bioRxiv - Biochemistry 2021Quote: A total of 50 μl of conditioned medium from ARPE-19 cell cultures were collected and used to quantify free fatty acids using the Free Fatty Acid Quantification Assay Kit (Colorimetric) (Abcam cat. # ab65341; Cambridge, MA) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Transiently transfected cells were stained the following day with mAbs conjugated using DyLight® 650 Conjugation Kit (Fast) - Lightning-Link (Abcam, catalog no. ab201803) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Tissue sections were stained for β-galactosidase using a Senescence Detection Kit (ab65351) according to the directions of the supplier (Abcam, Inc., Waltham, MA).
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bioRxiv - Biochemistry 2023Quote: ... Antibodies were fluorescently labelled with AF647 or AF488 using the Alexa Fluor® Conjugation Kit (Fast) - Lightning-Link (Abcam, Cat. No. ab269823 and ab236553) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The sample fractionation by protein size was conducted through an eight-channel GELFrEE® 8100 Fractionation System (GELFrEE® 8100 Fractionation System, 8% Cartridge Kit, #42103, Abcam, Cambridge, UK). The cartridge was prepared according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: The intracellular levels of ROS/RNS in different immune cells of CNS were evaluated using the Cellular ROS/RNS Detection Assay Kit (Abcam, USA; catalog number: ab139473) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonyl residues in were quantified in 10 μg of the cell lysates using the Protein Carbonyl Assay Kit (Abcam, Boston, MA, USA, ab178020). Derivatization and neutralization were carried out according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... To be included antibodies had to be available in a protein free format (except for GPS2, which we purified using an Abcam Protein G purification kit). All antibodies selected were known to be specific ...
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bioRxiv - Neuroscience 2020Quote: ... NAD and NADH extractions were performed on 20mg PBS washed fresh tissue on wet ice homogenised with a Dounce homogeniser with 400uL of NADH/NAD extraction buffer (Abcam NAD/NADH Assay kit-ab65348). For details ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated for 1 hour in the antibody incubation buffer (Massive Photonics, Buffer kit) with anti-guinea pig AlexaFluor 405 (Abcam, cat ab175678, 1:1000 dilution), phalloidin AlexFluor 488 (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... At 24 h post-infection flow cytometry was performed targeting NP: cells were processed with the BD Cytofix/Cytoperm™ Kit (catalog no. 554714) and stained with anti-NP antibody (Abcam, clone 9G8, catalog no. ab43821) followed by donkey anti-Mouse IgG Alexa Fluor 488 (Invitrogen catalog no ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 10 µl of the supernatant obtained after the first centrifugation at 18000g from the Glycogen assay where combined with 90 µl of Lactate assay Buffer (Abcam® L- Lactate Assay kit, #ab65331) and separated in two wells of a 96 well plate for sample and sample background control ...