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Citations for abcam :
1851 - 1900 of 2149 citations for Recombinant Human TNFRSF12A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... The membrane containing the purified proteins was probed with an anti 6X His Tag monoclonal antibody (Abcam; ab49746) conjugated to alkaline phosphatase at 1:2000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Precleared lysates (equal amounts of protein) were incubated with 3 µg rabbit polyclonal antibody to NrCAM (Abcam #24344) or nonimmune IgG (nIg ...
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bioRxiv - Bioengineering 2023Quote: ... Endogenous peroxidase and protein blocking steps were performed using reagents provided in the ABC detection kit (Abcam, ab64261). Sections were incubated overnight with primary antibodies at 4°C (Table S2) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed via ICC for the expression of smooth muscle protein 22-alpha (SM22A, Abcam, Cambridge, UK), ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2 ...
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bioRxiv - Microbiology 2023Quote: ... The immunohistochemistry technique was performed with a commercial antibody anti-SARS-CoV-2 spike protein (Abcam, Cambridge, UK) and the in-house mAb 1F7 ...
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bioRxiv - Pathology 2023Quote: ... antibodies against the following proteins were used to probe membranes: Collagen type I (0.9 µg/mL, ab138492, abcam), p-SMAD2 (0.1 µg/mL ...
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bioRxiv - Genomics 2023Quote: ... OXPHOS complex component proteins were probed for with an OXPHOS antibody cocktail (1:250, Abcam ab110412, RRID: AB_2847807). Protein levels were determined by band densitometry and quantified by normalizing to the most stable complex component (V).
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bioRxiv - Cancer Biology 2023Quote: ... AHA-labeled newly synthesized proteins were analyzed by electrophoresis and detected with anti-biotin antibody (Cat# ab201341, abcam). For visualizing total proteins ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared products were collected and incubated in fresh Protein A agarose beads and anti-huntingtin EPR5526 (Abcam) for 2 hours rotating at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were separated by SDS-PAGE and Western blots were performed with following antibodies: UCP1 (Abcam, #ab209483), PPARγ (Cell Signaling Techonology ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose® 4 Fast Flow beads (Cytiva) bound to 1 µg of anti-TRIB3 (abcam, ab75846) were incubated with 500 µg of cell lysate for 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were immunostained with anti-Arl13b (Protein Tech, 17711-1-AP or Abcam, ab136648; both 1:1000), anti-pericentrin (Abcam ...
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bioRxiv - Genetics 2024Quote: ... Equal volumes of SFB-tagged proteins in kinase reaction buffer and 500 µM ATP-γ-S (Abcam, ab138911) were mixed and incubated at 30°C for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... plasma sample at 10% in complete media was incubated with Protein A/G sepharose beads (Abcam, Boston, MA) for 1 hr with rotation at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM EDTA) and 1mg of protein lysate was subjected to IP with 5ug of anti-BRD4 antibody (Abcam, #ab128874) or anti-NFκB p65 antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... For subsequent interrogation of complement protein levels all samples were employed in either the C3 (Abcam ab108822 and 108823), iC3b ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were prepared in 1% donkey serum (in PBS) for the following proteins: GFP (ab13970, Abcam, 1:200), HA-tag (ab9110 ...
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bioRxiv - Cancer Biology 2021Quote: ... When using recombinant proteins such as PSME4 [purified as described in(Toste Rêgo and da Fonseca, 2019)] or PSME1 (AB-206168, abcam) 1 nM of each protein was add to 100 ul final reaction ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: ... lysates were diluted twofold in assay buffer and incubated with capture beads for FUT8 (protein G-agarose beads; Abcam) overnight ...
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bioRxiv - Biochemistry 2021Quote: ... and the purified protein band was also confirmed by Western blot analysis by using an anti-His antibody (Abcam).
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bioRxiv - Cell Biology 2021Quote: ... Supernatant (1 mg protein) was incubated overnight with rotation at 4°C with either nesprin-2 antibody (Abcam, #ab217057) or anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation was conducted using 50 seedlings for each sample with Dynabeads protein A and anti-myc (Abcam, Cambridge, MA). Western blots using anti-myc (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min and probed with mAb or pAb at 4°C overnight: anti-NPC proteins mAb (1:2,000, ab24609, abcam); anti-NUP98 pAb (1:2,000 ...
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bioRxiv - Neuroscience 2020Quote: ... and probed with antibodies against rabbit monoclonal growth associated protein-43 (GAP-43; 1:2000; #ab75810, Abcam, Cambridge, MA), mouse monoclonal neurofilament light polypeptide (NF-L ...
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bioRxiv - Biophysics 2020Quote: ... 1 mg Protein A dynabeads (2.8 m) were prepared and loaded with 10 g Anti-GFP antibody (Abcam ab290) or Anti-HaloTag (Promega G9281 ...
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bioRxiv - Biophysics 2020Quote: ... 1 mg Protein A dynabeads (2.8 m) were prepared and loaded with 10 μg Anti-RFP antibody (Abcam ab62341) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: 1 mg Protein G dynabeads (2.8 μm) were prepared and loaded with 10 g Anti-Digoxigenin antibody (Abcam ab6212) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for RNA-binding protein immunoprecipitation (RIP) assays: anti-H1.3 (ab24174, Abcam, 5 µg/reaction), anti-H1.5 (ab18208 ...
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bioRxiv - Biochemistry 2022Quote: ... co-immunoprecipitated proteins were eluted from the magnetic beads by incubating with 10 µL 4X LDS Sample Buffer (Abcam) at 70 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... protein expression was determined by Western blotting using as primary antibodies anti-FLAG or anti-V5 tag antibody (AbCam; 1:500 ...
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bioRxiv - Biophysics 2022Quote: ... The following primary antibodies were used to detect the presence of indicated proteins in this study: SETD3 (ab176582; 1:5,000; Abcam), Actin (A2066 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were analyzed by Western Blot using anti-UBF and anti-Top2a antibodies (sc-9131, Santa Cruz; ab12318, Abcam).
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... Primary antibodies and dilutions were as follows: anti-Wilms tumor protein antibody (WT1, 1:100, AB89901, Abcam, Cambridge, UK), anti-Meis2 antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... proteins (0.8-1 mg) were rotated overnight at 4°C with 5 µg of mouse anti-SGTa antibody (Abcam) or control mouse IgG and protein G Dynabeads (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... rat anti-CD45 (Becton Dickinson 550539, 1:200) and rat anti myelin basic protein (MBP, Abcam ab7349, 1:200). The following donkey-raised secondary antibodies (all in 1:400 dilution unless otherwise stated ...
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bioRxiv - Systems Biology 2020Quote: ... The primary antibodies against the following proteins were used in this study: ATP synthase complex (Abcam, Cambridge, MA, USA), KIF5B (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting nuclear extract was incubated with Dynal protein G beads which were preincubated with 5μg of H3K27me3 or H3 antibody (ab6002 and ab1791, Abcam) respectively at 4°C overnight ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibodies were used as follows: rat anti-glial fibrillary acidic protein (GFAP, 1:100, Abcam, Cambridge, MA, USA) for astrocytes and rat anti-CD11b (1:100 ...
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bioRxiv - Immunology 2021Quote: ... and the obtained values represent the ratio between the immunodetected protein and the glyceraldehyde 3-phosphate dehydrogenase (GAPDH, Abcam) loading control ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then incubated with a 10-fold molar excess of the chosen guanine nucleotide (GMPPNP or GDP (AbCam)) for 3 hours at room temperature (22 °C ...
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bioRxiv - Cell Biology 2021Quote: ... as determined by the staining of the SDS-PAGE gels with CBBR (InstantBlue Coomassie Protein Stain [ISB1L], Abcam: ab119211). Aliquots (10 μl ...
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bioRxiv - Cell Biology 2019Quote: Cytosolic and nuclear proteins were extracted from cultured NRVMs using the Nuclear Extraction Kit (Catalog. #: NC113474; Abcam, Cambridge, MA) according to the manual provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... patient serum was added to a 96-well MULTI-ARRAY plate (Meso Scale Discovery) coated with CD40 protein (Abcam) and allowed to bind at room temp for 2hr ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed twice with PBS containing 0.025% triton-x for 5 minutes and then blocked with protein block (ab64226, Abcam) for 20 minutes ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was used to visualize co-expression of certain proteins in glial or neuronal cell types: C3(Abcam 181147) and S100β (Abcam 41428) ...
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bioRxiv - Molecular Biology 2022Quote: ... HA-tagged proteins and histone H3 were detected with anti-HA (Abcam ab9110, 1:1,000 in 1% milk-PBST) and anti-H3 (Abcam ab1791 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in primary antibodies against the first protein of interest and tubulin (Abcam, Cambridge, UK,; 1:5000) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... IHC staining was applied to examine the expression of the protein encoded by Mpo (Myeloperoxidase, 1:1000, ab208670, Abcam) and Il1b (IL-1 beta ...
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bioRxiv - Bioengineering 2023Quote: ... The gels were washed once with distilled water and stained with InstantBlue® Coomassie Protein Stain (Abcam, Cambridge, UK). The gels were viewed using a ChemiDOC XRS molecular imager (Biorad ...