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Citations for abcam :
1801 - 1850 of 2313 citations for Recombinant Human EGFR Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: The detection of specific anti-RSV IgG antibodies in biological fluids was performed using Human Anti-Respiratory syncytial virus IgG ELISA Kit (ab108765, abcam®, Sweden), according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed once with 200 μL PBS containing 1% BSA and incubated with PE conjugated Goat F(ab’)2 Anti-Human IgG-(Fab’)2 secondary antibody (abcam, Cambridge, MA) at a final concentration of 2.5 μg/ml in 100 μL PBS containing 1% BSA per well ...
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bioRxiv - Microbiology 2020Quote: The IL-12p40 in cell supernatants of infected and uninfected macrophages were quantified using BD OpteEIA™ Human IL-12(p40) ELISA kit (Abcam, UK) as per the manufacturer’s directives.
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bioRxiv - Immunology 2020Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody (Abcam, ab98532, 1:2000) for 30 min at RT ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion was quantified in Liver-Chip effluent collected from the top channel using the Human Albumin SimpleStep ELISA® Kit (Abcam, #ab179887) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Expression of each species’ ACE2 receptor in the cells was confirmed by Western Blot analysis using antibody to human ACE2 (Abcam, Waltham, MA). Parental CRFK cells served as a control (Mock).
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bioRxiv - Microbiology 2021Quote: ... Chuncheon-si, ROK), anti-HCoV-OC43(LS-C79764, LS-bio, Seattle, WA, USA) and anti-human GAPDH antibody (ab9485, Abcam, Cambridge, UK) overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Cell Biology 2019Quote: The same protocol was used for the staining of VEGF-A expressing cells using a rabbit anti-human VEGF-A monoclonal antibody (ab52917, Abcam, Cambridge, UK), which was revealed using the same secondary antibody as above ...
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bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with heat-inactivated serum and visualized with a goat anti-human secondary antibody (IgG) conjugated with FITC (Abcam, Cat#ab97224).
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bioRxiv - Genetics 2020Quote: ... and incubated with rabbit polyclonal anti-PEA-15 (C-terminal amino acids 93-123 of Human PEA15) antibody ab135694 (Abcam, Cambridge, UK) at a concentration of 1:100 and anti-GAPDH antibody (MAB374 ...
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bioRxiv - Molecular Biology 2022Quote: ... blood samples from each repopulated mouse were collected via the saphenous vein and analyzed using the Human Albumin ELISA Quantitation Set (Abcam, Cambridge, UK) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for the western blot experiments: mouse monoclonal antibody against human apolipoprotein E (dilution 1:1000; Abcam, Cambridge, UK), and polyclonal rabbit antibodies against the LL37 epitope (diluted 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: Cell lysates were subjected to SDS/PAGE (0.1% w/v) immunoblotting analysis using an anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... washed three times with PBS and then 200 µl PBS containing anti-human antibodies conjugated to alkaline phosphatase (Abcam, 1:2000 dilution) was added for 1 h ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were removed and the plates were washed three times with PBS + 0.1% Tween20 before adding anti-human IgG HRP conjugate (Abcam, #ab97225, 1:20000) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were immunoprecipated from 1 μg chromatin and 1 μL of anti-H3 (Abcam, 1791) conjugated to 20 μl protein G magnetic beads (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Protein loading was also checked by performing Western blot using anti-beta Actin (Abcam, ab8227).
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or DNMT3A (DNA cytosine-5-methyltransferase 3A protein) (1:200 in PBS) (Abcam; ab23565, USA) at 4°C o/n ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were used against the following proteins: Erg (AF-647, Abcam ab196149, 1:100), Phospho-S6 Ribosomal Protein (Ser235/236 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were incubated overnight at 4 ℃ with anti-Surfactant Protein A antibody [6F10] (ab51891, abcam) diluted at 1:500 then probed the next day using goat anti-human IgG (IRDye 800 ...
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bioRxiv - Molecular Biology 2019Quote: ... and p85-wt-TAP and p85-A951E-TAP proteins were detected with anti-CBP (Abcam). Immunodetection of tubulin (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Proteins were transferred to polyvinylidene difluoride membrane and probed with primary antibodies (CASR (ADD, Abcam), AP2σ2 (Ab92380 ...
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bioRxiv - Neuroscience 2020Quote: ... double immunohistochemistry was performed with primary antibodies for glial fibrillary acidic protein (GFAP, abcam, ab4674) and ionized calcium-binding adaptor molecule 1 (IBA-1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... protein expression was assayed by Western blotting cell supernatant using anti-6xHis-HRP antibody (Abcam). Proteins from transient transfection were purified as follows ...
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bioRxiv - Bioengineering 2021Quote: ... the primary antibodies targeted: (1) glial fibrillary acidic protein (GFAP; 1/1000, goat, Abcam, ab53554), the key intermediate filament protein in astrocytes ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies against the following proteins were used: Phospho-MLKL (Ser345) (Abcam, Cat. No. ab196436); MLKL (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... followed by overnight at 4° C with rabbit anti-Maltose Binding Protein antibody (ab9084, Abcam) or rabbit anti-CPSF6 antibody (EPR12898 ...
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bioRxiv - Microbiology 2021Quote: ... The obtained proteins were separated by SDS-PAGE and immunoblotted using anti-GFP (ab290, Abcam), anti-H3 (ab1791 ...
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bioRxiv - Microbiology 2020Quote: ... IB analysis of the proteins was subsequently conducted using mAbs against myc and Flag (Abcam). RNase was added to the cell lysis and wash buffers.
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bioRxiv - Cell Biology 2020Quote: ... A protein concentration of 100 ug was loaded into each well and10μM Rhod-2AM (Abcam) dye diluted in 1% FBS-containing RPMI medium was added and incubated at 37°C for 30 minutes in order to stain the mitochondria ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein visualization was accomplished using anti-rabbit or anti-mouse HRP-conjugated secondary antibodies (Abcam) and Amersham™ ECL Prime Western Blotting Detection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibodies directed against the following proteins were: anti-ISG15 (1:3000 dilution; Abcam); anti-β-actin ...
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bioRxiv - Pathology 2022Quote: ... Dynabeads coated with protein G were incubated with either CAV1 antibody (5 μg, abcam, ab17052) or IgG mouse control antibody (5 μg ...
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bioRxiv - Bioengineering 2022Quote: ... Specific EV protein marker levels were quantified using western blot analysis for ALIX (Abcam ab186429) at 1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti SARS-CoV-1 and 2 Spike protein clone [1A9] monoclonal (Abcam, cat# ab273433) (1:200 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... Expression of proteins was assayed by western blot using antibodies against V5 tag (Abcam, ab9116), phospho-4E-BP1 (Thr37/46 ...
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bioRxiv - Cell Biology 2022Quote: ... Protein levels were analyzed by SDS-PAGE and immunoblotting using rabbit anti-Cav1 (ab2910, Abcam), rabbit anti-dynamin2 (PA1-661 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:1000 chicken anti-GFP (used for detection of CFP protein; Abcam, ab13970, RRID: AB_300798). Alexa-Fluor conjugated Donkey polyclonal secondary antibodies were from Molecular Probes/Life Sciences and used at 1:300 dilution.
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Coding and non-coding drivers of mantle cell lymphoma identified through exome and genome sequencingbioRxiv - Genomics 2019Quote: ... and we used Western blotting to observe HNRNPH1 protein levels (abcam ab10374, 1/10 000) and histone H3 protein levels (Cell Signaling Technologies #9715 ...
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bioRxiv - Plant Biology 2020Quote: ... ATG8a and actin protein levels were detected with anti-ATG8a antibody (1:1000; abcam, ab77003) and anti-actin antibody (1:3000 ...
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bioRxiv - Pathology 2020Quote: ... we performed immunohistochemical staining with antibodies for monocyte chemoattractant protein-1 (MCP-1, ab9669, Abcam), CD68 for macrophages (M0814 ...