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Citations for abcam :
1701 - 1750 of 1912 citations for Recombinant human IgE kappa L His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: NETs were quantified after IHC staining of 2.5 × 104 PMNs per well in a 96-well plate with mouse anti-human MPO antibody (Abcam) and rabbit anti-human citH3 antibody (Abcam) ...
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bioRxiv - Immunology 2020Quote: ... Then cells were washed twice with cold PBS and stained with mouse anti-human HLA-A2-FITC antibody (Abcam) for 30 min on ice ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody dilutions were prepared in 3% NGS in 1XPBS as follows: mouse anti-human vinculin (VIN-54, Abcam) at 1:200 and phalloidin conjugated with Alexa Fluor 546 (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended with 200 μl of 1%BSA-PBS and 0.25 mg/ml of PE-labeled goat anti-human IgG (Abcam), incubated in ice for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... eric.rubinstein{at}inserm.fr) and mouse IgG1 anti-human CD9 (clone TS9 1/100) (commercially available at Diaclone or Abcam) and then with the following secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Bead pellet was washed three times with 0.5% BSA in PBS before incubating in 1:200 rabbit anti-human NM23A (Abcam), NME2 (Abcam) ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated overnight at 4 °C with either an anti human frataxin monoclonal antibody (abcam, ab 110328), an anti HPRT monoclonal antibody (SC-376938) ...
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bioRxiv - Molecular Biology 2022Quote: ... Human NQO-1 (#ab28947) and HO-1 (#ab133064) protein level changes were quantified by using ELISA kits from Abcam.
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bioRxiv - Physiology 2021Quote: was performed on frozen sections (OCT embedded) of human vein segments using mouse anti-Collagen III antibody (ab7778, abcam). Briefly ...
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bioRxiv - Physiology 2020Quote: ... Desmin polyclonal rabbit anti-human antibody (Cat# ab15200, RRID: AB_301744) was used (1:200) from Abcam (Abcam, Cambridge, UK), and secondary antibody (TRITC polyclonal goat anti-rabbit ...
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bioRxiv - Neuroscience 2021Quote: 16 μg of protein extracted from frozen human tissue samples was employed in a hemoglobin sandwich ELISA (Abcam Ab157707). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: Levels of NT3 protein in serum and muscles were assessed using a Human Neurotrophin-3 ELISA Kit (#ab100615 Abcam). In this study the viral vector encoded the human prepro-NT3 gene ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 nM followed by detection with a goat anti-human Fc FITC secondary antibody (Abcam ab97224, Cambridge, UK) at 1:200 and fixation in 4% formalin ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... analyses was performed as described in manufacturer’s protocol (Double Stain IHC Kit: M&R on human tissue; DAB & AP/Red; Cat # ab210059; Abcam).
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bioRxiv - Immunology 2020Quote: ... HT29 (human colorectal adenocarcinoma grade II) whole cell lysate was used as a positive control for TMPRSS2 (Abcam, ab3952). Full length predicated at 54 kDa and the cleavage fragment at 25 ...
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bioRxiv - Genetics 2021Quote: ... the paraffin section of human embryonic brown adipose samples was successively stained with UCP1 antibody (Abcam, ab10983; 1:500), Perilipin antibody (CST ...
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bioRxiv - Microbiology 2020Quote: ... The exact content of ACE2 in NPs was determined with a human ACE2 ELISA kit (ab235649, Abcam, Shanghai, CHN). ELISA was conducted in triplicate and repeated three times.
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bioRxiv - Immunology 2021Quote: ... incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (1:5,000 in 1% nonfat dry milk-TBST; Abcam) for 1 h at RT and visualized with KwikQuant Ultra digital ECL substrate using the KwikQuant digital imager (Kindle Biosciences ...
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bioRxiv - Cell Biology 2021Quote: The amounts of secreted prolactin were quantified in the cell supernatants by the Prolactin Human ELISA Kit (Abcam, USA). The data were normalized to the total amount of protein determined by the Bradford method ...
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bioRxiv - Immunology 2022Quote: Total human IgG in the collected culture medium was measured by the quantitative SimpleStep ELISA Kit (ab195215, ABCam, UK) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Circulating levels of ACE2 from plasma samples were quantified using the Human ACE2 SimpleStep ELISA® Kit (#ab235649, Abcam) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 54 subjected to Sodium dodecyl-sulfate polyacrylamide gel electrophoresis and stained with rabbit anti-human CTHRC1 antibody (Abcam, ab85739). Fibronectin detected using an anti-human antibody (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: Immunohistochemistry (IHC) for CDK12 expression was performed with polyclonal rabbit-anti-human-CDK12 antibody (ab246887; Abcam; dilution 1:50) using the protocol described in supplementary methods section ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation overnight at 4°C with a human-specific mouse anti-nestin antibody (abcam, ab22035) in the blocking solution ...
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bioRxiv - Microbiology 2023Quote: ... mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1) p24 clone [39/5.4A] (#ab9071, Abcam, dilution 1:500). Mouse monoclonal anti-Human Immunodeficiency Virus Type 1 (HIV-1 ...
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bioRxiv - Cell Biology 2023Quote: The human RTK phosphorylation assay was performed using an antibody array membrane accordingly to the manufacturer specifications (ab193662, Abcam).
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Duke University] and GAPDH (mouse anti-human GAPDH, 1:10,000, 1 hr at RT; Cat: ab8245, Abcam, Waltham, MA). Primary antibodies were detected using donkey anti-rabbit IRDye® 800CW IgG (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Membrane was then incubated in 1:5000 primary antibody (HRP-conjugated Goat pAb to human IgG, Abcam, cat.: 7153) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were then washed three times in PBS 0.05% Tween 20 and then incubated at room temperture with horseradish peroxidase conjugated secondary antibody diluted in PBS 0.05% (For X509: Goat-anti-human (Abcam), 1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... samples were incubated with one of the following primary antibodies (anti-PECAM-1, rabbit anti-human, cat#AB32457, Abcam, 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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bioRxiv - Bioengineering 2022Quote: ... and incubated for 1h at room temperature with anti-mouse or anti-rabbit IgG H&L secondary antibodies conjugated with Alexa Fluor™ 488 or Alexa Fluor™ 568 (goat, 1:500; Abcam). Slides were mounted with VECTASHIELD antifade mounting medium containing DAPI (Vector Labs ...
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bioRxiv - Biophysics 2022Quote: ... The cells were washed with PBS three times before labeling with goat anti-rabbit IgG H&L Biotin (ab97049; abcam, Cambridge, UK) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were washed 3x with PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed 3x with 100 μL PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at room temperature and a secondary antibody (1:500; Donkey anti-mouse IgG H&L, Alexa Fluor® 568, Abcam #ab175472) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... anti-occludin antibody (ab216327, and goat anti-rabbit IgG H&L (Alexa Fluor 488, ab150077) antibody were all obtained from Abcam (Waltham, MA). Anti-tumor necrosis factor-α (aTNF ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Bioengineering 2023Quote: ... the sections were incubated with secondary gt-α-rb IgG H&L antibody (Alexa Fluor® 488) (Abcam, ab150077, 1:500 dilution) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA); Ro 3306 ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody solutions were added for 1 hour at room temperature: donkey anti-rabbit IgG H&L (Alexa Fluor 647) at 1:200 (Abcam, Cambridge, Massachusetts); donkey anti-mouse IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Immunology 2023Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...