Labshake search
Citations for abcam :
1701 - 1750 of 2079 citations for 6 Chloro 4 iodopyridin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Slides were then incubated in a humidified chamber overnight at 4°C with primary antibodies (1:50 dilution): rabbit anti-human CD20 (ab78237, Abcam), rat anti-mouse Fibroblast Marker ER-TR7 (sc73355 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Where antibody staining was required coverslips were permeabilized with 0.2% Triton X-100 for 10 min and blocked in 4% BSA in PBS for 20 min followed by incubation with relevant antibodies (DHRS7 abcam at 1:1,000 dilution or rabbit lamin A antibodies [17] at 1:50 dilution for 1 hr at RT ...
-
bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were then stained overnight at 4°C with primary antibodies against anti-CD3 (ab52959, Abcam, CA, USA), anti-CD4 (AF-379-NA ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were then incubated overnight at 4°C on a shaker (65 rpm) with primary antibodies as follows: anti-CD206 (Abcam) for macrophages and anti-CD31 (Dako ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
-
bioRxiv - Immunology 2023Quote: ... followed by incubation at 4◦C overnight with primary antibodies diluted in the blocking buffer (rabbit anti-Dclk1 1:1000, Abcam, ab31704 ...
-
bioRxiv - Genomics 2023Quote: ... Chromatin was incubated overnight at 4°C with antibodies to lamin B1 (10 μg per 10 million cells; Abcam, ab16048), coupled to Invitrogen Dynabeads protein A/G (Thermo-Fisher) ...
-
bioRxiv - Immunology 2023Quote: ... The membranes then were incubated at 4◦C overnight with the primary antibodies diluted in the blocking buffer (rabbit anti-NLRP3 1:1000, Abcam, ab263899 ...
-
bioRxiv - Immunology 2023Quote: ... the specimens were incubated overnight at 4 °C with 5µg/mL rabbit anti-GFP antibody (ab290, Abcam, Cambridge, MA, USA). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: ... Sections were then washed in deionized water and blocked with a solution of 5% normal goat serum (NGS) in PBS for 15 min at 37 °C and then stained overnight at 4 °C with 4G2 antibody (Abcam) at 1:200 ...
-
bioRxiv - Neuroscience 2024Quote: ... membranes were incubated overnight at 4°C with the following primary antibodies at the indicated dilutions: anti-BMAL1 (Abcam (UK), ab93806) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Debris were removed using centrifugation and the chromatin was incubated overnight at 4°C with antibody against H3K9me3 (Abcam, Ab8898). Antibody-bound chromatin was captured using magnetic beads (Themo Fisher ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.1% BSA pH 7.4 in phosphate buffered saline (PBS) or Tris-HCl buffered saline (TBS) and incubated at 4°C overnight with primary antibodies (anti-Nr4a2, Abcam, ab41917 ...
-
bioRxiv - Microbiology 2024Quote: ... at 1:10,000 followed by incubation with either secondary goat pAb to mouse IgG HRP (ab97040, Abcam, Lot # GR3219575-4) at 1:10,000 or secondary mouse antibody to rabbit IgG HRP (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2024Quote: ... and blocked with 5% milk in TBS-Tween 20 for 2h and incubated with primary antibodies diluted in the 5% BSA in TBS-Tween 20 overnight at 4°C (AMPD2, Sigma HPA045760, 1:1000; IMPDH2, Abcam ab129165 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4°C with a mix of primary antibodies: chicken anti-GFAP (1:500, Abcam ab4774) and rabbit anti-GFP (1:1000 ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated for 48 h with primary antibody in PBST (containing 0.3% Triton X-100) on a 4°C agitator using rabbit anti-c-fos (1:8000, Abcam, USA) antibody ...
-
bioRxiv - Cancer Biology 2023Quote: ... were sonicated and incubated overnight under rotation at 4 °C with 5 ug/ml of anti-RNAPII-Ser2p (Abcam, ab193468) or anti-IgG (Cell Signaling ...
-
bioRxiv - Biochemistry 2024Quote: ... Membranes blocked for 1 h at room temperature were probed overnight at 4 °C in 5% non-fat dry milk with 1:2,000 anti-CA (Abcam ab63917) or 1:5,000 anti-IN77 ...
-
bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
-
bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with the following primary antibodies in 5% skim milk at 4 °C overnight: rabbit anti-ATF4 antibody (ab270980, 1:1000, Abcam), XBP1 antibody (ab37152 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated for 72 hours at 4°C with the following dilutions of the primary antibodies in blocking buffer: Pecam1 1:50 (ab28364, Abcam), Keratin 8 1:100 (Troma1 ...
-
bioRxiv - Cell Biology 2019Quote: The following primary antibodies were used for immunofluorescence imaging (at the indicated final concentration diluted in 3% BSA in PBS): chicken α-GFP (ab13970 from Abcam, 1:5000), guinea pig α-Cenp-C (BT20278 from Caltag + Medsystems) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
-
bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
-
bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).
-
bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
-
bioRxiv - Bioengineering 2023Quote: ... The slices were then incubated overnight at 4°C with primary antibody solutions (supplemented with 3% (v/v) Triton X-100 in 1× PBS) to target astrocytes (GFAP) and neuronal nuclei (NeuN) (Abcam Inc., Cambridge, UK) (Table 1) ...
-
bioRxiv - Cell Biology 2023Quote: ... gravid worms were placed in 1 drop of ddH2O containing 3% (v/v) pelleted OP50 and 100 μM bafilomycin A1 (Abcam, dissolved in DMSO) for 16 h at 20 ℃ ...
-
bioRxiv - Cancer Biology 2021Quote: ... carried out overnight on a rotating wheel at 4 °C in the presence of 20 μg of anti-V5 antibody (Abcam, ab9116). 2.5% of the input sample was collected just before addition of the antibody and stored at −20 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 4°C with one of the following mouse monoclonal primary antibodies: anti-heavy chain cardiac myosin (Abcam, #ab50967), anti-α-actinin (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections were blocked with 5% donkey serum and incubated overnight at 4°C against MMP13 (1:100, Abcam ab39012, Cambridge, UK) and for 2 hours at room temperature with a Cy3-conjugated secondary (1:500 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400 ...
-
bioRxiv - Developmental Biology 2020Quote: ... at 4 °C overnight followed by staining with a secondary goat-anti-rabbit antibody coupled with Alexa 568 (ab175471, Abcam, US).
-
bioRxiv - Developmental Biology 2020Quote: ... tissue sections were blocked with 2% Bovine Serum Albumin in PBS for 1 h and then incubated overnight at 4 °C with the following antibodies: ISLET-1 (1:200, abcam, ab109517), HES1 (1:200 ...
-
bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21: ab88572, Abcam; anti-GFP: ab290, Abcam). Paired-end sequencing was performed on a NovaSeq6000 platform (Illumina ...
-
bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
-
bioRxiv - Molecular Biology 2019Quote: ... and subsequently incubated with primary antibody overnight at 4°C (1:1000 anti-CTCF C-terminus Millipore 61311 and 1:2000 anti-TBP Abcam ab51841) in Odyssey blocking buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in 4% NGS containing an antibody targeting αsyn phosphorylated at serine 129 (anti-pSer129, rabbit monoclonal, 1:600, Abcam #51253). Subsequently ...
-
bioRxiv - Bioengineering 2022Quote: ... The following primary antibodies were used at a 1:200 dilution (as recommended by the manufacturer) and incubated overnight at 4°C: anti-αSMA (Abcam ab5694) or anti-Rac2 (Fischer Scientific ...
-
bioRxiv - Microbiology 2019Quote: ... at room temperature for 120 min and then incubated overnight at 4°C with primary antibodies for caspase-9 (Cat # ab69514, Abcam USA), caspase-3 (Cat # ab90437 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Embryos were then blocked at room temperature for 1 hour in 10% heat-inactivated goat serum and then stained with the following affinity-purified primary antibodies overnight at 4°C: anti-β-Catenin antibody (1:500; ab6302, Abcam), anti-Cdh1 (1:200 ...
-
bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight at 4°C in a humidified chamber with primary antibodies against chicken GFP (1:1000; Abcam #ab13970) and rabbit aromatase (1:100 Abcam #ab18995) ...
-
bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4 °C with Protein G magnetic beads (Dynabeads: ThemoFisher) that had been pre-incubated with anti-H3K27Ac (Abcam, ab4729) or anti-Sox2 (R&D ...
-
bioRxiv - Neuroscience 2019Quote: ... for 30 min at 4°C in the presence of protease inhibitors (Pierce™. protease inhibitor Mini Tables, Pierce Biotechnology Inc) and PMSF 1M (Abcam). Protein concentration was determined using the bradford protein assay (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2019Quote: ... They were then incubated overnight at 4°C with mouse monoclonal antibody against tyrosine hydroxylase (TH) (1:750, Abcam, Cambridge, UK) or goat polyclonal primary antibody against cathepsin X (1:200 ...
-
bioRxiv - Molecular Biology 2019Quote: ... coverslips were blocked with 4% BSA/PBS for 45 min at RT and primary antibody anti-rabbit lamin B1 (abcam ab16048), mab414 (1:500 ...