Labshake search
Citations for abcam :
1651 - 1700 of 1719 citations for Phosphatidylinositol PI CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Protein lysates from each subcellular fraction (cytoplasm, mitochondria, and nucleus) were obtained from PNT1 and LNCaP cells using the cell fractionation kit (Abcam, VIC, Australia) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: Enzyme activity in vaginal swab supernatants and cell pellets was quantified using the α-Glucosidase Activity Assay Kit (Abcam, Waltham, MA, USA), Enzchek Ultra Amylase Kit (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Conjugation to FITC or ammonium peroxidase was carried out using Abcam’s alkaline phosphatase and FITC conjugation kits (Abcam ab102850 and ab102884 respectively) according to the protocols outlined in the manufacturer’s instruction manuals ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... The organic phase was evaporated under liquid nitrogen and lipids were resuspended in 50µL of fatty acid assay buffer (Free Fatty Acid Assay Kit, Abcam, Cambridge, MA, USA). FA (contained in 5µL of buffer ...
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bioRxiv - Microbiology 2019Quote: GAPDH activity was measured in the presence and absence of GAs using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Colorimetric Assay Kit (ab204732, Abcam, Cambridge, MA) as per the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained using the EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit (Abcam, United States; Cat. no. ab80436). The enzyme expression was visualized with the mouse monoclonal antibody anti-SOD2 (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
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bioRxiv - Neuroscience 2020Quote: Absolute quantification of sialic acid in different glycoconjugate fractions was performed using the Sialic Acid (NANA) Assay Kit (Ab83375, Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... equal number of cells were lysed using assay buffer provided in the alpha KG assay kit (abcam, ab83431, lot no. GR3410863-1). The lysates were then deproteinized using perchloric acid and nutralized using 2M KOH as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Total protein levels were measured by BCA assay (Pierce) and a 1:00 dilution was used for quantifying α-synuclein using the mouse α-synuclein ELISA kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Two microliter aliquots of the supernatants were transferred to wells of a 96-well plate prefilled with 98μl of assay buffer of the ammonia assay kit from AbCam (AbCam Cat # ab102509), and ammonia levels were quantified according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... serotonin and brain derived neurotrophic factor using commercially available Enzyme linked immunosorbent assay kits according to the instructions of the manufacturer (ABCAM, Cambridge UK)
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Biochemistry 2023Quote: Right Ventricular Mitochondrial Enrichments: RV mitochondrial enrichments were isolated from the same PAB and control piglets with a mitochondrial isolation kit (Abcam, Cambridge, MA) as previously described(10 ...
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bioRxiv - Bioengineering 2023Quote: ... was dissected from the constructs at the end of maturation time (day 15) and tested via a colorimetric CK Activity Assay Kit (Ab155901, Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... regularly spaced (500 µm) along the rostrocaudal axis were stained with a Prussian blue and nuclear fast red stain kit (Abcam, Cambridge, UK) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: RCC243 cells were treated with 5 μM MS023 for the indicated time periods and then cells were trypsinized and stained using the Propidium Iodide Flow Cytometry Kit (Abcam, #ab139418, Abcam) based on the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... None of the cultures or treatments showed signs of cell cytotoxicity using the using the LDH-Cytotoxicity Assay Kit II (Abcam, cat. #ab65393) to analyze 50 ul fresh culture media (not shown) ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Physiology 2024Quote: Mitochondrial membrane potential was measured in γY4KD cells with Pparγ silenced with siRNA and their scrambled control (Scrl) using Mitochondrial Membrane Potential Assay kit (Abcam, Cat# ab113852). Cells were plated on collagen coated 24-well plate at density of 50,000 cells/well (5 well/per group) ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: D2HG levels were measured in adherent cultures of 1 x 107 chondrosarcoma cells treated with increasing concentrations of Enasidenib for 48 hours using the D2HG colorimetric Assay Kit (Abcam, Cambridge, UK) following the manufactureŕs recommendations.
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bioRxiv - Synthetic Biology 2023Quote: ... listed in Supplemental Table 2 were used to quantify TrxB expression levels with the GFP ELISA kit (Abcam, Cambridge, UK, Cat# ab171581) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... were used to quantify the ratio of reduced (GSH) to oxidized (GSSG) circulating glutathione with a commercial ELISA kit (Abcam; Cambridge, UK) as an indicator of oxidative stress levels and redox imbalance.33
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Physiology 2020Quote: ... and IL-10 concentrations were measured using ELISA kits (TNF-α: R&D Systems, Minneapolis, MN, USA; IL-1β, IL-6, and IL-10: Abcam), in accordance with the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Neuroscience 2023Quote: ... Triton X-100 in 1X PBS for 20 min and then endogenous biotin was blocked using Avidin/Biotin blocking kit (Abcam; cat. no. ab64212) to reduce non-specific background ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Molecular Biology 2023Quote: The main pro-inflammatory and anti-inflammatory cytokines in blood and heart were measured by corresponding ELIZA kit: tumor necrosis factor-ɑ (TNF-ɑ, Abcam, ab100747), interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...