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Citations for abcam :
1601 - 1650 of 2070 citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 0.5% BSA and 2 mM EDTA added to Anti-GFP antibody (1:500, Abcam, Ref. ab6556) for 15min at 4-8°C ...
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bioRxiv - Neuroscience 2023Quote: ... a rat monoclonal against tyrosinated α-tubulin (clone YL1/2, Abcam, Cat. Number: ab6160, RRID: AB_305328) diluted 1:2000 ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... cells were subjected to ChIP assay with primary antibody against ESR1 (2 μl/IP, Abcam #ab32063) or normal rabbit IgG (1 μg/IP ...
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bioRxiv - Immunology 2023Quote: ... mtDNA was extracted from 2×107 of Mito+ RBCs with the Mitochondrial DNA Isolation Kit (Abcam) and quantified with Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... or 2) rabbit Tau (D1M9X; 1:600) and mouse GAD-67 (Abcam, Ab 26116; 1:200) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were given daily intraperitoneal injections of either DMAPT (0.2, 2, or 20 µg/kg; Abcam; dissolved in DMSO and further diluted in sterile PBS ...
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bioRxiv - Biochemistry 2023Quote: ... at room temperature and incubated with 1:1000 rabbit anti-LRRK2 monoclonal antibody (Abcam c41-2) diluted in 1% BSA O/N at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Biotin-conjugated goat anti-human IgG (Fab’)2 antibody (Abcam, Waltham, MA, USA; 1 μg/ml) was subsequently added for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The antibody to tyrosinated α-tubulin was added first using rat monoclonal antibody YL1/2 (Abcam), diluted 1:200 with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 h with Chicken IgY-Alexa Fluor 488 (1:500, Cat #150169, Abcam) in 0.4% Triton-PBS ...
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bioRxiv - Bioengineering 2024Quote: ... The blocks were sectioned onto slides and stained with anti-neutrophil Ly6B.2 antibody (Abcam, ab53457). Histological images were analyzed by a blinded individual ...
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bioRxiv - Bioengineering 2024Quote: ... and a saturation step (45 min, RT) with goat serum (2%; Abcam Inc., Cambridge, MA, USA) and bovine serum albumin (5% ...
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bioRxiv - Genomics 2024Quote: ... washed and pre-incubated with 2 µg of primary antibody (CTCF: Diagenode, C15410210-50; SMC1A: Abcam, ab9262 ...
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bioRxiv - Biochemistry 2024Quote: ... Five-micrometer sections of colon tissues were stained with Alcian Blue or Mucin-2 (Abcam #ab272692), examined using a ZEISS Axio Observer microscopy platform (Carl Zeiss Microscopy ...
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bioRxiv - Developmental Biology 2024Quote: ... chromatin was IP’d overnight at 4°C with 2 μg of anti-YBX1 antibody (Abcam, ab12148) pre-bound to Dynabeads Protein G (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).
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bioRxiv - Bioengineering 2023Quote: ... The slices were then incubated overnight at 4°C with primary antibody solutions (supplemented with 3% (v/v) Triton X-100 in 1× PBS) to target astrocytes (GFAP) and neuronal nuclei (NeuN) (Abcam Inc., Cambridge, UK) (Table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed 3 times in 1x PBS for 10 minutes then incubated in blocking solution containing AlexaFlour488 conjugated secondary antibodies (Abcam, ab150077; 1:1000) for 2-4 hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for western blotting at the indicated dilutions: anti-CD63 (MBL, D263-3, 1:5000; Abcam, ab134045, 1:2000), anti–Flotilin-1 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... gravid worms were placed in 1 drop of ddH2O containing 3% (v/v) pelleted OP50 and 100 μM bafilomycin A1 (Abcam, dissolved in DMSO) for 16 h at 20 ℃ ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were incubated for 3 h at room temperature with a mix of secondary antibodies (ThermoFisher A-11039 or Abcam ab150158, 1:400) that had been previously centrifuged at 20000g for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: Apoptosis and proliferation levels were measured by immunostaining in dewaxed and rehydrated midline placental sections with cleaved caspase-3 (Asp175) (Cell Signalling, US, 9661; 1:200) and Ki67 (Abcam, UK, ab264429; 1:500). Sections were incubated with goat-anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing both WGA-555 (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475, Lot GR3234297-32) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times with 1x PBS and placed in secondary (1:500 AF488 conjugated Goat anti-Rabbit (Abcam, Cambridge, UK, Cat# AB150077), 1:500 Cy3 conjugated Donkey anti-mouse (Jackson Immuno Research Labs ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then treated with 3% bovine serum albumin in PBS and incubated overnight at 4°C with the primary mouse antibody anti-Pax8 (Abcam ab53490, diluted 1/100). After washing unbound antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Following primary antibody incubation brains were washed 4 times for 15 minutes each in a 3% salt solution (in 1X PBST) and transferred to secondary antibodies (Abcam: ab175673; ThermoFisher: A-21236) in 1XPBST and incubated overnight at 4°C on a nutator ...
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bioRxiv - Cancer Biology 2023Quote: ... The lower part of the membrane was blocked with 3% BSA in phosphate-buffered saline (PBS) solution followed by incubation with rabbit polyclonal anti-GFP (Abcam, UK; #ab290, 1:1,000) primary antibody at RT for 1 h for eGFP detection (30 kDa) ...
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bioRxiv - Neuroscience 2024Quote: Western blot analysis was performed on hCMEC/D3 cell lysates using antibodies against full-length and cleaved caspase-3 (Abcam, ab32351, 1:500 dilution), VE-Cadherin (Cell Signalling ...
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bioRxiv - Plant Biology 2024Quote: ... Membranes were washed in TBS-T for 3 × 10 minutes incubating with secondary anti-rabbit (Cell Signalling Technology catalogue # 7074S; 1:2,000) and anti-mouse (Abcam Ltd. Catalogue # ab6728; 1:5,000) in TBS-T with 5% milk for one hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... except that brains were incubated overnight in apoptosis-related primary antibodies (H2A.X, 1:500, EMD Millipore, 05-636, made in mouse; and caspase 3, 1:500, abCam, AB13847, made in rabbit) overnight in blocking buffer at 4°C with gentle rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 20% milk in PBST for 1h following primary antibody incubation (COX-2: Abcam, Cat# ab15191 ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: rabbit anti-Ki67 (1:200; 2 hours at room temperature; Abcam, Ab16667), rabbit anti-β-catenin (1:300 ...
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bioRxiv - Genomics 2021Quote: ... We used the following antibodies in this work: anti-Pol II-total (ab817, lot GR3271868-2, Abcam), anti-Pol II-S5 (ab5131 ...
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bioRxiv - Neuroscience 2020Quote: ... The efficiency of knockdown was assessed by immunostaining using the LRRK2 antibody [MJFF2 (C41-2)] (# ab133474, Abcam) at1:200 dilution ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... In this case 400 μgr of PNS were incubated with 2 μg of anti-GFP (Abcam, ab1218). Following Western blot ...
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bioRxiv - Microbiology 2020Quote: 2 µl of EV isolates and two fold serial dilutions were spotted onto a nitrocellulose membrane (Abcam) and process for dot blot using anti-EV polyclonal murine serum (8 ...
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bioRxiv - Cancer Biology 2022Quote: Protein expression of CXCL2 (MIP-2) was quantified using an enzyme-linked immunosorbent assay (ELISA; Abcam ab184862). Cell culture supernatant was collected after 24 hours and ELISA was performed following the manufacture’s protocol.
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bioRxiv - Immunology 2022Quote: ... Sections were then covered with rabbit anti-SARS-CoV-2 N protein monoclonal antibody (Abcam; 1:1000) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The following antibody dilutions were used: rabbit polyclonal anti-Aconitase 2 antibody at 1:1000 (ab83528; Abcam), rabbit polyclonal anti-6X His tag antibody at 1:1000 (ab1187 ...
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bioRxiv - Immunology 2022Quote: ... incubated with 0.2 μg/mL of anti-SARS-CoV-2 spike glycoprotein antibody (abcam, Catalog No. ab272504) for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... Then the organoids were incubated for 2 hours at RT with primary antibodies against cytokeratin 8 (Abcam, Cambridge ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348 ...
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bioRxiv - Genetics 2020Quote: ... 2 μg of purified mRNAs were mixed with 5 μg of anti-m A antibody (Abcam, ab151230) in 450 μL immunoprecipitation buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated in 2% milk with the corresponding secondary horseradish peroxidase-conjugated antibodies (1:10.000, Abcam) for 1 hour at room temperature ...
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bioRxiv - Physiology 2021Quote: ... washed 2 × 5 min in PBS and then incubated with primary antibodies against Laminin-α2 (#11576, Abcam) in antibody solution containing 10% goat serum in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with α-mouse IgG-AP conjugated (1:2000 in 2% BSA/PBSTx) (ab5880 Abcam, Cambridge, UK) at 4°C overnight ...