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1551 - 1600 of 4776 citations for Recombinant Mouse Enpp7 His tagged since 2019

• Healing of Primary and Recurrent Mouse Ocular Herpetic Disease Following Topical Ocular Treatment with an Engineered Fibroblast Growth Factor-1 (FGF-1) is Associated with Increased Frequency and Function of Corneal Anti-Inflammatory M2 Macrophages

  Nisha R Dhanushkodi, et al., bioRxiv - Immunology 2020

  Quote: ... the corneas were incubated overnight with a cocktail of rabbit anti-mouse A488 conjugated anti-mouse LYVE-1 (Abcam), PE-conjugated anti-mouse CD31 (Millipore) ...
• Universal method for the gentle isolation of intact microvessels from frozen tissue: a multiomic investigation into the neurovasculature

  Marina Wakid, et al., bioRxiv - Neuroscience 2023

  Quote: ... and the washed pellet was resuspended in 250 ul of the following antibody solution: recombinant Alexa Fluor® 647 anti-ERG (1:100 dilution; ab196149, Abcam, Massachusetts, United States) in 0.5% BSA + 0.1% Triton in PBS ...
• Early defects in lysosomal storage diseases disrupt excitatory synaptic transmission

  Camila de Britto Pará de Aragão, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti-mouse postsynaptic density protein 95 (PSD-95, 1:1000 for cells, 1:200 for tissues, Abcam, catalog ab99009), rabbit anti-mouse vesicular gamma aminobutyric acid (GABA ...
• The rates of adult neurogenesis and oligodendrogenesis are linked to cell cycle regulation through p27-dependent gene repression of SOX2

  Ana Domingo-Muelas, et al., bioRxiv - Neuroscience 2022

  Quote: ... antibodies (1:100 mouse anti-ASCL1, BD 556604; 1:100 mouse anti-ASCL1, Guillemot lab; 1:800 rat anti-BrdU, Abcam ab6326 ...
• Dual blockade of misfolded alpha-sarcoglycan degradation by bortezomib and givinostat combination

  Lucile Hoch, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C overnight for the mouse anti-KDEL 1:100 (ADI-SPA-827, Enzolife) and the mouse anti-LAMP2 1:500 (ab25631, Abcam). Washing was carried out 3 times for 5 minutes at room temperature with PBS and then secondary antibodies alexa fluor 488 goat anti-mouse 1:1000 (A-11029 ...
• Caveolae couple mechanical stress to integrin recycling and activation

  Fidel-Nicolás Lolo, et al., bioRxiv - Cell Biology 2022

  Quote: ... Total cell lysates were separated by SDS-PAGE and analyzed by western blot with rabbit monoclonal anti-mouse Caveolin-1 and rabbit polyclonal anti-mouse PTRF (Abcam), with mouse monoclonal anti tubulin used as the loading control ...
• Tissue-specific MicroRNA Expression Alters Cancer Susceptibility Conferred by a TP53 Noncoding Variant

  Qipan Deng, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Tissue sections were developed using the rabbit-specific HRP/DAB (ABC) Detection IHC Kit or Mouse on Mouse Polymer IHC Kit (Abcam). After haematoxylin counterstaining ...
• Knockout of Cyp26a1 and Cyp26b1 during post-natal life causes reduced lifespan, dermatitis, splenomegaly and systemic inflammation in mice

  Jessica M Snyder, et al., bioRxiv - Pathology 2020

  Quote: The RBP4 concentrations in mouse serum and liver were quantified using a mouse RBP4 ELISA kit (ab202404; Abcam; Cambridge, UK) according to the manufacturer’s instructions ...
• ATRX alteration contributes to tumor growth and immune escape in pleomorphic sarcomas

  Lucie Darmusey, et al., bioRxiv - Cancer Biology 2021

  Quote: ... tissue sections were blocked with 5% mouse serum PBS1X for 1h30 and incubated with mouse anti-tryptase antibody (1:300, ab2378, RRID:AB_303023, ABcam, Cambridge, UK) for 1h at RT ...
• STAT3 localizes in mitochondria-associated ER membranes instead of in mitochondria

  Yixun Su, et al., bioRxiv - Cell Biology 2019

  Quote: ... the cells were incubated in appropriate secondary antibodies (goat-anti-rabbit-AF488, goat-anti-mouse-AF568 or goat-anti-mouse-AF647 (Abcam), both diluted at 1:200 in the blocking buffer ...
• Voluntary running does not increase capillary blood flow but promotes neurogenesis and short-term memory in the APP/PS1 mouse model of Alzheimer’s disease

  Kaja Falkenhain, et al., bioRxiv - Neuroscience 2020

  Quote: ... Sections were then incubated overnight at 4°C with primary antibodies against Iba1 (1:500, rabbit anti-mouse Iba1; 019-19741, WAKO) and GFAP (1:500, chicken anti-mouse GFAP; ab53554, Abcam) in the same buffer ...
• The CD103-XCR1 axis mediates the recruitment of immunoregulatory dendritic cells after traumatic injury

  Ravi Lokwani, et al., bioRxiv - Bioengineering 2022

  Quote: The XCL-1 measurement in mouse blood plasma samples was performed by using Mouse XCL- 1 SimpleStep ELISA kit (Abcam). The assay was performed as per manufacturer guidelines ...
• N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules

  Pavel Kudrin, et al., bioRxiv - Molecular Biology 2023

  Quote: ... Primary antibodies (rabbit anti-ac4C, #ab252215, Abcam; rabbit anti-NOP58, #14409-1-AP, Proteintech; mouse anti-NCL, #87792, CellSignal; mouse anti-G3BP, #ab56574, Abcam) were diluted 1:50 in blocking solution and incubated with the samples for 1 hr at RT ...
• Scribble, Lgl1, and myosin IIA interact with α/β-catenin to maintain epithelial junction integrity

  Maha Abedrabbo, et al., bioRxiv - Cell Biology 2023

  Quote: ... Horseradish peroxidase-conjugated goat anti-Mouse (ab6789) and donkey anti-Mouse IgG H&L (Alexa Fluor 555) (ab150110) were from Abcam. All antibodies were diluted 1:1000 for Western blot and 1:100 for immunofluorescence.
• Encoding of the colorectal cancer metabolic program through MICU2

  Alison Robert, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibody was mouse anti-pimonidazole and the secondary antibody was FITC-labeled goat anti-mouse (Abcam, Cambridge, USA).
• SNAP25 disease mutations change the energy landscape for synaptic exocytosis due to aberrant SNARE interactions

  Anna Kádková, et al., bioRxiv - Neuroscience 2023

  Quote: ... Incubation in primary antibodies (a-SNAP25: mouse, 1:10000, SYSY 111011, Synaptic Systems; a-VCP: mouse, 1:2000, ab11433, Abcam) was performed overnight with 70 rpm shaking at 4 °C ...
• Long-term potentiation in neurogliaform cells modulates excitation-inhibition balance in the temporoammonic pathway

  Marion S. Mercier, et al., bioRxiv - Neuroscience 2021

  Quote: ... using mouse anti-reelin (1:1000; Abcam, ab78540) and goat anti-mouse Alexa Fluor 568 (1:500 ...
• A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

  Kevin B. Weyant, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... anti-mouse IgG1 (1:10,000; Abcam Cat # ab97240), or anti-mouse IgG2a (1:10,000 ...
• A modular platform for on-demand vaccine self-assembly enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens

  Kevin B. Weyant, et al., bioRxiv - Synthetic Biology 2021

  Quote: ... HRP-conjugated goat anti-mouse (Abcam; Cat # ab6789) was used as needed ...
• Single-cell landscapes of primary glioblastomas and matched organoids and cell lines reveal variable retention of inter- and intra-tumor heterogeneity

  VG LeBlanc, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Mouse anti-Carbonic Anhydrase (CA9) (Abcam; 1:500), Rabbit anti-Myelin Basic Protein (BMP ...
• Chlamydia coinfection inhibits HPV-induced safeguards of the cellular and genomic integrity in patient-derived ectocervical organoids

  Stefanie Koster, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse-anti-Msh2 (3A2B8C) (1:1000, Abcam, # ab52266), rabbit-anti-Mlh1 (EPR3894 ...
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... HRP conjugated mouse anti-hnRNP K (Abcam; ab204456), HRP conjugated rabbit anti-ILF3 (Abcam ...
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... and HRP conjugated mouse anti-Nucleophosmin (Abcam; ab202579). For control experiments ...
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... and HRP conjugated mouse anti-Nucleophosmin (Abcam; ab202579).
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... HRP conjugated mouse anti-hnRNP K (Abcam; ab204456), HRP conjugated rabbit anti-ILF3 (Abcam ...
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... and HRP conjugated mouse anti-Nucleophosmin (Abcam; ab202579).
• Highly sensitive in situ proteomics with cleavable fluorescent tyramide reveals human neuronal heterogeneity

  Renjie Liao, et al., bioRxiv - Systems Biology 2020

  Quote: ... HRP conjugated mouse anti-hnRNP K (Abcam; ab204456), HRP conjugated rabbit anti-ILF3 (Abcam ...
• Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19

  Cheng Dong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... anti-SCP3 (mouse monoclonal, 10G11/7, ab97672; Abcam), anti-γ H2A.X (mouse monoclonal ...
• EIPR1 controls dense-core vesicle cargo retention and EARP complex localization in insulin-secreting cells

  Irini Topalidou, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse monoclonal anti-proinsulin (1:100, Abcam, #ab8301), rabbit polyclonal anti-TGN38 (1:350 ...
• Centrosome and ciliary abnormalities in fetal akinesia deformation sequence human fibroblasts

  Ramona Jühlen, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-γ-tubulin (1:1000; ab11316; Abcam), mouse anti-β-tubulin (1:1000 ...
• The eIF2α kinase HRI triggers the autophagic clearance of cytosolic protein aggregates

  Mena Abdel-Nour, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-α-synuclein (#ab1903, Abcam 1/3000), Ubiquitin ...
• Using Expansion Microscopy to visualize and characterize the morphology of mitochondrial cristae

  Tobias C. Kunz, et al., bioRxiv - Cell Biology 2020

  Quote: ... and α-GFP (ab1218, mouse; ; Abcam, Cambridge, UK). All primary antibodies were used in a dilution of 1:100 ...
• The structural basis for kinetochore stabilization by Cnn1/CENP-T

  Stephen M. Hinshaw, Stephen C. Harrison, bioRxiv - Cell Biology 2020

  Quote: ... and rabbit anti-mouse IgG-HRP (Abcam 97046).
• Defining totipotency using criteria of increasing stringency

  Eszter Posfai, et al., bioRxiv - Cell Biology 2020

  Quote: ... and mouse anti Krt18 1:100 (ab668, Abcam). To visualize trophoblast cells in placentas ...
• Distinct roles for RAB10 and RAB29 in pathogenic LRRK2-mediated endolysosomal trafficking alterations

  Pilar Rivero-Ríos, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse monoclonal anti-GAPDH (1:2000; Abcam, ab9484), mouse monoclonal anti-flag (1:500 ...
• Partial rescue of neuronal genes deregulated in Cornelia de Lange Syndrome by cohesin

  Felix D. Weiss, et al., bioRxiv - Cell Biology 2020

  Quote: ... and mouse monoclonal NeuN (1:1000, Abcam, ab104224). Samples were then washed with PBS and incubated in secondary antibodies diluted 1:500 in staining solution (0.1% Triton X-100 ...
• DNAJB chaperones inhibit aggregation of destabilised proteins via a C-terminal region distinct from that used to prevent amyloid formation

  Shannon McMahon, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-SQSTM1/p62 (1:2000; ab56416, Abcam), mouse anti-ubiquitin (1:1000 ...
• BNIP3 phosphorylation by JNK1/2 promotes mitophagy via enhancing its stability under hypoxia

  Yun-Ling He, et al., bioRxiv - Cell Biology 2020

  Quote: ... anti-p62 (1:10,000, mouse mAb, Abcam, ab56416), anti-phospho-BNIP3 (Ser 60 ...
• The ESCRT protein CHMP5 restrains skeletal progenitor cell senescence by preserving endo-lysosomal-mitochondrial network

  Xianpeng Ge, et al., bioRxiv - Cell Biology 2020

  Quote: Mouse anti-OXPHOS antibody cocktail (cat#ab110413, Abcam)
• Tryptophan residues in TDP-43 and SOD1 mediate the cross-seeding and toxicity of SOD1

  Edward Pokrishevsky, et al., bioRxiv - Cell Biology 2020

  Quote: ... and 1:100 mouse anti-HA (Abcam, ab130275). After washes with PBSTw ...
• APEX-Gold: A genetically-encoded particulate marker for robust 3D electron microscopy

  James Rae, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti mouse IgG (1:3000, Abcam ab97046), rabbit α-tubulin (1:3000 ...
• A TIR-1/SARM1 phase transition underlies p38 immune pathway activation in the C. elegans intestine

  Nicholas D. Peterson, et al., bioRxiv - Immunology 2021

  Quote: ... and anti-mouse IgG secondary antibodies (Abcam, #ab6789) were diluted 1:10,000 and used to detect the primary antibodies following the addition of ECL reagents (Thermo Fisher Scientific ...
• Mechanical collaboration between the embryonic brain and the surrounding scalp tissues

  Koichiro Tsujikawa, et al., bioRxiv - Developmental Biology 2021

  Quote: ... anti-cytokeratin 10 (mouse, ab9025, Abcam, 1:500), anti-elastin (rabbit ...
• Behavioural and molecular characterisation of the Dlg2 haploinsufficiency rat model of genetic risk for psychiatric disorder

  Sophie Waldron, et al., bioRxiv - Genetics 2021

  Quote: ... and mouse anti-GAPDH (1:5000, Abcam, UK). Membranes were then subject to 3 × 10 minute TBST washes before incubation with the appropriate fluorescent IRDye 680RD secondary antibodies at 1:15,000 dilution in 5% milk at room temperature ...
• The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double strand break repair

  Tao Huang, et al., bioRxiv - Developmental Biology 2020

  Quote: ... mouse anti-SCP3 (1:500 dilution; Abcam #ab97672), rabbit anti-SCP1 (1:2,000 dilution ...
• Meiotic nuclear architecture in distinct mole vole hybrids with Robertsonian translocations: chromosome chains, stretched centromeres, and distorted recombination

  Sergey Matveevsky, et al., bioRxiv - Evolutionary Biology 2020

  Quote: ... mouse antibodies MLH1 (1:50, Abcam, Cambridge, UK), mouse anti-phospho-histone H2AX (1:1000 ...
• Schistosoma Japonicum infection in Treg-specific USP21 knock-out mice

  Youxiang Zhang, et al., bioRxiv - Immunology 2020

  Quote: ... abcam)and HRP-Rabbit anti-Mouse IgG(ab6728, abcam) were used as the secondary antibody (1:20,000 ...
• Mechanistic Determinants of Slow Axonal Transport and Presynaptic Targeting of Clathrin Packets

  Archan Ganguly, et al., bioRxiv - Neuroscience 2020

  Quote: ... and anti-Mouse IgG (ab190475; Abcam, Cambridge MA) for overnight coupling with magnetic beads at 37°C ...
• Unexpected opposing biological effect of genetic risk factors for Parkinson’s disease

  Marcus Keatinge, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse α-synuclein antibody (abcam ab27766, 1:1000), mouse LAMP1 antibody (Novus Biologicals NPB2-25155 ...
• Acquisition of cellular properties during alveolar formation requires differential activity and distribution of mitochondria

  Kuan Zhang, et al., bioRxiv - Developmental Biology 2021

  Quote: ... mouse anti-MTCO1 (1:100, abcam, Cat# ab14705), chicken anti-GFP (1:300 ...