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Citations for abcam :
1551 - 1600 of 2180 citations for Recombinant Human GAB1 Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Antibodies were as follows: i) for human cells: mouse IgG1 anti-CD44/CD73/CD90/CD45/CD105/CD14/HLA-DR (all from Abcam); ii ...
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bioRxiv - Microbiology 2021Quote: ... Beads were next washed twice with 200 μl wash buffer and resuspended in 100 μl FITC-conjugated rabbit anti-human C3c polyclonal antibody (Abcam) diluted 1:500 in BB and incubated in the dark at 25°C for 20 min ...
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bioRxiv - Physiology 2021Quote: ... Schiaffino and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa Department of Biology as well as laminin (#11575; Abcam) and 10% goat serum ...
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bioRxiv - Cancer Biology 2020Quote: ... The stainings were performed using either CDK6 monoclonal rabbit anti-human antibody (Clone EPR4515, dilution 1:250, Abcam, Cambridge, UK) or Cyclin D2 monoclonal rabbit anti-human antibody (Clone D52F9 ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion by HepG2 cells encapsulated in liver models with or without vasculature channels was quantified using ELISA (Human Albumin ELISA kit, Ab 108788, Abcam). Liver models (polymer composition ...
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bioRxiv - Cancer Biology 2021Quote: ... based on hematoxylin and eosin (H&E) staining and nuclear immune-positivity for human-specific NCL (ab13541, Abcam, Cambridge, UK) as previously described [21] ...
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bioRxiv - Cell Biology 2019Quote: ... RUNX-2 positive cells were similarly stained using a mouse anti-human RUNX-2 monoclonal antibody (ab76956, Abcam, Cambridge, UK), which was revealed using an Alexa Fluor®488 goat anti-mouse IgG2a secondary antibody (A-21131 ...
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bioRxiv - Bioengineering 2019Quote: Levels of brain natriuretic peptide (BNP) secreted in the media were quantified by Human BNP ELISA Kit (Abcam, cat# ab193694) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Immunology 2020Quote: ... day 1st-stimulated CAR T cells were co-cultured with γ-irradiated target cells at a 1:3 E:T ratio in the presence of 10 μg/ml plate-bound human CD226 blocking monoclonal antibody (clone DX11, Abcam, UK). Ultra-LEAF™ purified mouse IgG1(Clone MOPC-21 ...
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bioRxiv - Molecular Biology 2021Quote: Human RIPK3 rat monoclonal 1H2 (generated in-house)(Petrie et al, 2019b) Human phospho-MLKL (Ser358) rabbit monoclonal ab 187091 (Abcam) VDAC1 rabbit polyclonal AB10527 (Millipore)
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bioRxiv - Immunology 2020Quote: ... The following fluorochrome conjugated rabbit anti-human antibodies were also used in the intracellular staining: FITC anti-GLUT3 (polyclonal) and PE anti-GLUT1 (EPR3915) from Abcam. Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Immunology 2020Quote: Cytokine levels in the cellular supernatant were evaluated using the FirePlex-HT assay system with the Human Cytokines FirePlex-HT Panel 1 (ab234897; Abcam). FirePlex-HT immunoassays quantify up to 10 protein analytes per sample from low sample inputs in 384-well plate format ...
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bioRxiv - Microbiology 2020Quote: ... then incubated with 0.1μg/ml HRP-conjugated Goat Anti-Human IgG H&L or HRP-conjugated Goat Anti-Rabbit IgG H&L (abcam) in 0.5% BSA in PBST for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Cancer Biology 2020Quote: ... rehydrated through an alcohol gradient to water and antigen retrieval in citrate buffer pH 6.0 was used for the human nucleolin antibody at 5.0 g/mL (ab13541) (Abcam, Canbridge, MA). Endogenous peroxide activity and nonspecific binding was blocked with 3%(v/v ...
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bioRxiv - Bioengineering 2022Quote: ... for detection (brown signals) of collagen type I (1:100 anti-human collagen I alpha-1 1200-1450 fragment (ab233080, Abcam) compatible to pig elastin [45] ...
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bioRxiv - Genomics 2022Quote: We determined the cytomegalovirus (CMV) serostatus of AFB (n = 80) and EUB (n = 80) donors with a human anti-IgG CMV ELISA kit (Abcam) on plasma samples ...
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bioRxiv - Microbiology 2022Quote: ... tween 20 in PBS (PBST) three times followed by incubation with goat F(ab’)2 anti-human IgG (Fab’)2-horseradish peroxidase (Abcam) at a 1:10,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-human MT1-MMP rabbit monoclonal antibody EP1264Y against the catalytic domain (Abcam, catalog no. ab51074, 1:1000 dilution) were used followed by anti-rabbit secondary antibodies (Li-COR ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies (Ab) used in this study: rabbit polyclonal Ab to human serum albumin (Abcam, catalogue no. ab2406, 1:400), goat polyclonal antibody to E-cadherin (R&D systems ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The human origin of all patient-derived organoid xenografts (PDOXs) was confirmed by staining with an antibody recognizing human mitochondria (ab92824, Abcam).
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bioRxiv - Immunology 2022Quote: ... Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG/M antibody (Abcam) for 1h ...
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bioRxiv - Systems Biology 2023Quote: ... PVDF membranes were blocked and stained with total OXPHOS human western blot antibody cocktail (Abcam Cat# ab110411, RRID:AB_2756818, 1:500). Blots were visualized using an Odyssey infrared imaging system (RRID:SCR_022510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then incubated in a humidified chamber overnight at 4°C with primary antibodies (1:50 dilution): rabbit anti-human CD20 (ab78237, Abcam), rat anti-mouse Fibroblast Marker ER-TR7 (sc73355 ...
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bioRxiv - Cancer Biology 2023Quote: ... Labelling of c-Myc positive cells was performed with human c-Myc antibody (Abcam rabbit ab#32072 dilution 1:200) and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633) ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Synthetic Biology 2023Quote: The serum concentration of His-tagged PRL N59D was determined using a quantitative ELISA as follows: 50ul of 0.1ug/mL anti-human PRL antibodies (Abcam; ab244037) were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates for 1hr at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... and stained with anti-human type II or I collagen antibody (1:1,000, clone II-4C11, ICN Biomedicals, Germany; or Abcam, Germany), followed by biotinylated goat anti-mouse antibody (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:1500 (abcam, ab218108, Cambridge, UK). Envision labeled polymer (Dako ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:500 (abcam, ab218108, Cambridge, UK). The infiltration of human T lymphocytes into the neoplastic tumors was assessed using anti-human CD3 at 1:500 (abcam ...
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bioRxiv - Immunology 2024Quote: ... Primary anti-human TSHR antibody was used for 60 minutes at room temperature at 1:1500 (abcam, ab218108, Cambridge, UK). Slides were subsequently prepared as described in the immunohistochemistry section.
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bioRxiv - Molecular Biology 2024Quote: ... Samples were centrifuged at 1,000 g for 30 minutes before proceeding to ELISA (Human FAS Ligand ELISA Kit - CD95L –Abcam-ab100515).
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bioRxiv - Cell Biology 2024Quote: ... Membranes were probed with the following primary antibodies: rabbit polyclonal anti-calreticulin (1:1000) (ab19261, abcam; to detect human CRT); chicken polyclonal anti-calreticulin (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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bioRxiv - Biochemistry 2024Quote: ... Wells were then washed with TBST (0.1% tween 20) and then incubated with an anti-human IgG antibody conjugated to HRP (Abcam, ab102420) for one hour at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: Primary Abs used for these studies were specific for: myelin basic protein (MBP, Abcam), CD45 (Abcam) ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with EV71 VP1 protein specific mouse monoclonal antibody (clone 10F0; Abcam), and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L ...
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bioRxiv - Biochemistry 2019Quote: Anti-GP ab88078 is a mouse mAb purified by protein-A chromatography (Abcam, P.L.C.). The immunogen was residues 734–843 of human muscle GP (UniProt reference sequence P112176) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-myelin proteolipid protein (PLP; 26 and 30 kDa; 1:1000; Abcam), mouse monoclonal anti-myelin basic protein (MBP ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were rat anti-myelin basic protein (MBP; 1:500; Abcam, Cambridge, UK), rabbit anti-glutathione S-transferase π (GSTπ ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against green fluorescent protein (GFP, 1:2000, Abcam, USA), and mCherry (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were obtained from the via FASP Protein Digestion Kit (#ab270519, Abcam, Cambridge, MA), using trypsin (Proteomics Grade ...