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Citations for abcam :
1551 - 1600 of 2151 citations for Recombinant Human DHPS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Samples were then stained with rat anti-myelin basic protein (#ab7349, Abcam, 1:100), rabbit anti-GFAP (#ab7260 ...
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bioRxiv - Cancer Biology 2023Quote: ... GDF15 (Protein-tech, 27455-1-AP; 1:500) and FOXP3 (Abcam, ab215206; 1:1000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... protein bands are visualized by re-staining the same gels by Coomassie staining (AbCam) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and chicken anti-microtubule-associated protein 2 (MAP2) antibody (Abcam, Cat# ab92434, 1:70)] ...
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bioRxiv - Microbiology 2023Quote: Antibodies to the following proteins were used in this study: EEA-1 (Abcam, ab2900), LAMP-2 (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP) for astrocytes (1:500, ab279290, Abcam, UK), rabbit anti-neuronal nuclei (NeuN ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 15 μL protein A magnetic beads and 2 μL V5 antibody (Abcam, Catalog # ab9116) were added to the mixture followed by rotation at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... GFP -fused proteins were detected using rabbit monoclonal anti-GFP antibody (1:3000; Abcam). The blot signal was detected by chemiluminescence image analysis system (Tanon-5200).
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bioRxiv - Microbiology 2023Quote: ... Antibodies against specific proteins were used as follows: GPx4 (1:1000, Abcam Cat# ab125066), SLC7A11 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble nuclear and insoluble nuclear proteins were isolated using a commercial kit (#ab219177, Abcam) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The gel was stained with 25 mL of InstantBlue Coomassie Protein Stain (Abcam, ab119211) for 15 min at RT and protein bands of interest were excised ...
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bioRxiv - Cancer Biology 2024Quote: Immunoprecipitation assays were performed using the mitochondrial protein IP kit from Abcam (catalog # ab239710) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... biotin and biotin-binding proteins was blocked with Avidin/Biotin Blocking Kit (abcam, ab64212) in those sections where botinylated secondary antibodies were used ...
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bioRxiv - Physiology 2024Quote: ... The primary antibodies were rabbit antibodies targeting AMP-activated protein kinase (AMPK; Abcam, ab207442), adipose triglyceride lipase (ATGL ...
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bioRxiv - Neuroscience 2021Quote: ... Microglia were probed with a 24h incubation at 4°C with either a rabbit antihuman IBA1 antibody diluted in blocking solution (1:200, 019-19741, Wako chemicals, USA) or a goat anti-human IBA1 antibody (1:200, ab5076, Abcam, UK). We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... surface expression of iNKG2D was correlated with GFP expression using a MicAbody and detecting with PE-anti-human kappa chain (Abcam #ab79113) or by directly conjugating the Rituximab-MicAbody to Alexa Fluor 647 (Alexa Fluor Protein Labeling Kit #A20173 ...
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bioRxiv - Neuroscience 2020Quote: ... The MJFF rabbit monoclonal Rab12 pSer105 (equivalent phosphorylation site to human pSer106) antibody was described previously [21] and is available from Abcam (ab256487). Recombinant anti-LRRK2 pSer1292 MJFR-19-7-8 (ab203181) ...
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bioRxiv - Microbiology 2020Quote: ... peroxidase-labeled (Cat. 04-10-06, KPL, USA) or goat anti-human IgA alpha chain (alkaline phosphatase) (Cat. Ab97212, Abcam, UK), diluted 1:1000 in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were used: C-terminus (aa 450-499) of human Cdc20 (C-term Ab, 1:500, Abcam, ab26483), N-terminus (aa 1-175 ...
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bioRxiv - Cell Biology 2021Quote: ... cut based on the molecular weights indicated by the prestained marker and then incubated overnight on a shaker at 4 oC with rabbit anti-human STEAP3 (1:1000) (abcam, 151566), mouse anti-human p53 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: Supernatant was harvested from HeLa tBID2A cells as described above and used to probe a Human Growth Factor Antibody Array (Abcam, ab134002) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Immunology 2021Quote: ... This spin was repeated and re-suspended in 200 μL of CBB containing FITC rabbit anti-human C3c pAb (Abcam, UK) a 500-fold dilution ...
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bioRxiv - Immunology 2021Quote: ... Grids were permeabilized and blocked with 0.1% Saponin/1% cold-water fish skin gelatin (Perm buffer) for 10 min and first stained with anti-human CD63 (Abcam, ab59479) antibody (1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... Human and mouse lung sections were stained for colocalization with primary and secondary antibodies: ANT1 (Abcam #ab102032 rabbit polyclonal, 1:1000), pan ANT (anti-ANT1/2/3 Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse monoclonal anti-human-ATM(phospho-S1981) which stains the homologous mouse ph-ATM(phosphor-S1987) was used at 1:500 (Abcam ab36810). The slides were incubated in biotin-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h and then incubated overnight at 4 °C with primary antibodies: rabbit anti-human IRS2 (ab134101; Abcam, Cambridge, UK) and mouse anti-human active β-catenin (05-665 ...
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bioRxiv - Cell Biology 2022Quote: ... Amphiregulin concentrations in HT-22 cell fractions were determined by using a Human Amphiregulin ELISA kit (ab222504; detection range: 18 – 600 pg/mL; Abcam, Netherlands). Protein concentrations were measured using RC DC Protein Assay (5000120 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated with primary antibodies O/N at 4°C (Rabbit anti-human Lyve-1, Fitzgerald; Goat anti-murine Lyve1, R&D AF2125; rabbit anti-CD31, abcam Ab28364) and washed three times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then processed for immunostaining by overnight incubation at 4°C in the primary antibodies of anti-Human phospho alpha-synuclein (Ser129) (1:2000, Cat# ab51253, Abcam, RRID:AB_869973), anti-Iba-1 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... was performed on the plasma samples from the donors obtained from the blood bank using Human Anti-Cytomegalovirus IgG ELISA kit (abcam, #ab108724) according to the manufacturer’s protocol to check for the donors’ sero-status ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with PBSF and resuspended in 200 μL PBSF with 1:1000 goat anti-human antibody conjugated to AlexaFluor647 (Abcam, ab190560) and incubated for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (50 μm) of skin were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam) at 1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (10 μm) of DRG were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Physiology 2023Quote: ... and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM)21,22 were used to study the effects of α-MSH (abcam, # ab120189) and selective MC-R agonists in vitro ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain, IF 1:150) was from Abcam (#ab21679). Mouse monoclonal anti-clathrin light chain (clone X22 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies and dilutions for human muscle cross section imaging: Donkey anti-mouse (Alexa Fluor® 488) 1:500 (Abcam, #ab150105), donkey anti-goat (Alexa Fluor® 594 ...
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bioRxiv - Cancer Biology 2024Quote: The evaluation of human MMPs (Matrix Metalloproteinases) and TIMPs (Tissue Inhibitors of Metalloproteinases) was conducted following the instructions provided by the manufacturer (abcam 134004). In summary ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were immunoprecipated from 1 μg chromatin and 1 μL of anti-H3 (Abcam, 1791) conjugated to 20 μl protein G magnetic beads (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... Protein loading was also checked by performing Western blot using anti-beta Actin (Abcam, ab8227).
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or DNMT3A (DNA cytosine-5-methyltransferase 3A protein) (1:200 in PBS) (Abcam; ab23565, USA) at 4°C o/n ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies were used against the following proteins: Erg (AF-647, Abcam ab196149, 1:100), Phospho-S6 Ribosomal Protein (Ser235/236 ...