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Citations for abcam :
1551 - 1600 of 3239 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Each well of a 6-well plate was incubated for 30min at room temperature with 200μL of Cell Extraction Buffer PTR (ab195215, ABCam, UK) containing phosphatase inhibitors and the protease inhibitor aprotinin ...
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bioRxiv - Bioengineering 2023Quote: ... and plated into a Matrigel-coated 96-well plate with mTESR media containing ROCK inhibitor Y-27632 (10 uM, Abcam) at 30,000 cells per well one day before transfection ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG/M antibody (Abcam) for 1h ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed again and bound C3d or C5b-9 detected using a monoclonal anti-C3d antibody (1:500, Abcam) or a monoclonal anti-C5b-9 antibody against the neoepitope (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... This was done using a commercially available luminescence-based kit (ADP/ATP ratio kit, Abcam, cat.no. ab65313) following the user manual suggested by the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat. Ab28364, Abcam, Cambridge, UK) diluted 1:50 in the same blocking buffer ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Zoology 2020Quote: ... 0-3 days old) and older buds (stage 3, 1 month old) by two different means: anti Phospho-histone H3 (PHH3) immunolocalization (Abcam ab5176) antibody (1:200 ...
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bioRxiv - Microbiology 2019Quote: Immunostaining for p62 and ubiquitin was performed with blocking and permeabilization buffer (1% saponin, 3% bovine serum albumin) and anti-p62 rabbit monoclonal antibodies (Abcam #AB109012) at a concentration of 1 μg/ml or anti-ubiquitylated protein antibodies (clone FK2 ...
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bioRxiv - Physiology 2019Quote: ... samples were incubated overnight at 4°C with the following antibodies diluted in 3% BSA blocking solution and 1% horse serum: Anti-cTnT (Abcam, ab33589), anti-cTni (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The the membranes were incubated overnight at 4°C with rabbit anti-VEGFR-3 antibodies (Abcam, Cambridge, MA, USA; 1:50). Horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit antibodies (Sigma-Aldrich Co. ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Developmental Biology 2019Quote: ... After washing 3 times with TBS-T the blots were incubated with HRP-conjugated secondary antibodies (goat-anti-mouse HRP (Abcam, ab6789); goat-anti-rabbit HRP (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Tendon stromal cells isolated from healthy volunteers and patients with tendon tears (n=3 each) were incubated with the following primary antibodies: anti-ALX (Abcam, ab26316), anti-ALOX15 (Abcam ab119774) ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Cell Biology 2020Quote: ... the ovarian samples were incubated overnight at 4°C with primary antibodies against caspase-3 (1:1000 dilution, Abcam, Cambridge, MA) and NLRP3 (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... Slices containing VPMpc were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... The sections were incubated overnight with anti-cell marker cluster of differentiation (CD) 3 antibody (1:200, Abcam, ab16669, Cambridge, UK) at 4°C in a humidified incubation chamber ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in PBS with 3% donkey serum and the following primary antibodies for 72 h at 4°C: goat anti-Iba1 (1:500; Abcam, ab5076), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3 to 7 days on X-tra® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546 ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Microbiology 2023Quote: 10 µm-thick paraffin sections from juvenile and adult Chinook salmon brains (n=3/group) were deparaffinized and treated with trypsin antigen retrieval solution (Abcam, ab970) at room temperature for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... washed twice with 3 % BSA and incubated for 1 h with monoclonal rabbit anti-Ki-67 antibody (Abcam, ab16667, 1:1000) and washed three times with PBS ...
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bioRxiv - Immunology 2024Quote: ... sections were repaired using EDTA antigen recovery buffer and incubated overnight at 4 °C with anti-dengue virus 1+2+3+4 primary antibody (Abcam, ab26837). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Neuroscience 2020Quote: ... NBQX disodium salt (NBQX, 5 μM) and CGP 55845 hydrochloride (CGP, 4 μM) were obtained from Abcam. Fluorocitrate was prepared as previously described (Paulsen et al. ...
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bioRxiv - Genetics 2021Quote: ... or blocked in 5%NGS/PBST for 1hr and incubated overnight with THSB4 (Abcam, ab211143, 1:100) and α-Myosin Heavy Chain (F59 ...
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bioRxiv - Physiology 2022Quote: ... dantrolene (1 µM from a stock concentration of 5 mM in DMSO, to stabilise ryanodine receptors, Abcam), streptomycin (50 µM from a stock concentration of 15 mM in distilled water ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μg of lysate was incubated with 5 μg of either polyclonal rabbit anti-SBDS (Abcam, ab128946) or polyclonal rabbit anti-RPS19 (Bethyl-A304-002A ...
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bioRxiv - Microbiology 2021Quote: ... T- REx-293-EV cells were treated with (+)-JQ1 (5 µM, Abcam, Cat# ab141498, dissolved in DMSO) or the equivalent amount of DMSO [0.025% (v/v)] 30 min before TNF-α stimulation ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA and probed with primary antibodies overnight (anti-THEM6, ab121743, anti-CALR, ab22683, Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were incubated with the anti-p53 antibody PAb 240 (5 μg/ml; Abcam, Cambridge, UK), overnight at 4 °C ...