Labshake search
Citations for abcam :
1551 - 1600 of 1874 citations for 2 3 Cyclohexenyl Ethyltrimethoxysilane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and QH1 antibody (Hybridoma Bank, AB_531829, 1:200),or (2) anti-laminin rabbit polyclonal antibody (Abcam, ab11575, 1:250) and QH1 antibody (Hybridoma Bank ...
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bioRxiv - Neuroscience 2024Quote: ... The AMPA receptor mediated currents were recorded at -70 mV for at least 2 min of stable baseline and then blocked with 6,7-dinitroquinoxaline-2,3(1H,4H)-dione (DNQX, Abcam, ab120169) 40 µM ...
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bioRxiv - Bioengineering 2024Quote: ... No. 30-2300) were purchased from ATCC. 4,6-diamidino-2-phenylindole (DAPI) (Cat. No. ab228549) was purchased from Abcam. EGFP mRNA-lipid nanoparticle (LNP ...
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bioRxiv - Synthetic Biology 2024Quote: ... MSD GOLD 96-well streptavidin SECTOR plate was coated with 2 μg/mL biotinylated anti-C5b-9 antibody (Abcam) and blocked with PBS containing 2% BSA 0.05% Tween 20 (v/v) ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 hrs at room temperature on an orbital shaker before being incubated in the primary antibody (1:1,000, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in 1% non-fat milk in TBST overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... ARGX-119 was detected using a HRP-conjugated goat F(ab’)2 anti-human IgG-Fc polyclonal antibody (Abcam) for 1h at RT followed- by addition of the TMB substrate ...
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bioRxiv - Neuroscience 2024Quote: ... Then primary antibodies were applied in PBS containing 0.3% Triton and 2% NGS: chicken anti-GFP (1:1000; ab13970, Abcam), rabbit anti-RFP (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were rinsed TBS and nuclei were counterstained with 0.5 µg/mL of 4’,6-Diamidino-2-phenylindole (DAPI; Abcam) at RT for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were held at a constant 100 V for 2 h prior to staining with InstantBlue Coomassie Stain (AbCam).
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bioRxiv - Cell Biology 2024Quote: ... membranes were stripped (Thermo; cat# 21059) and re-blotted with anti-alpha-actinin 4 (Abcam EPR2533[2]; rabbit monoclonal) and anti-GFP (Millipore 11811460001 ...
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bioRxiv - Biophysics 2024Quote: ... The cleared supernatant was then incubated with 3 µg anti-myc antibody (SIGMA M4439 monoclonal anti c-myc or Abcam ab206486 rat mAb to myc tag (9E10)) for 1.5h at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were blocked in 20% milk in PBST for 1h following primary antibody incubation (COX-2: Abcam, Cat# ab15191, 1:1000 dilution, 1h at room temperature in 1% BSA; CYP2E1: Abcam, Cat# ab28146 ...
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bioRxiv - Systems Biology 2022Quote: ... and the supernatant containing chromatin was incubated with 20 μg of antibody (INO80 antibodies, abcam cat # ab105451, lot GR3206182-2) overnight at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and 2% NGS (block) for 1h at room temperature before incubation in primary antibodies (rabbit anti-Synapsin I, ab64581, Abcam; guinea pig anti-GLT-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Immunology 2020Quote: ... 6 µg of pre-cleared chromatin was immunoprecipitated with 2 µg/IP anti-Histone H3K27Ac (Abcam Cat#ab4729; RRID: AB_2118291), 4 µg/IP of anti-LXRα/β (provided by Knut Steffenssen ...
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bioRxiv - Molecular Biology 2021Quote: ... blocked in 2% normal donkey serum/1% BSA in PBS and incubated overnight with guinea pig anti-insulin (1:500, Abcam) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibodies against Rad53 (1:4000; rabbit, ab104332, Cell Signalling) or Tubulin (1:2000; rat, YL1/2, Abcam) diluted in TBS-T containing 1% BSA for 1 h 30 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...
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bioRxiv - Immunology 2021Quote: ... plus either rabbit polyclonal anti-SARS-CoV-2 Spike/RBD (Sino, 40592-T62) or rabbit polyclonal anti-CD11b (Abcam, ab52478) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with non-fat dry milk and incubated overnight with primary antibodies at 4°C (Abcam polyclonal rabbit IgG: Anti-MMP2 – ab37150, Anti-MMP3 – ab53015, Anti-MMP9 – ab38898, Anti-COX-2 – aDb15191 and Abcam mouse monoclonal anti-beta III Tubulin IgG2a – ab78078 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Neuroscience 2021Quote: ... Lysis buffer (930 µl) and 2 µg of either JADE1 antisera or anti-0N tau antisera (EPR21726, Abcam, Cambridge, MA) were combined and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Thermo Fisher Scientific Baltics UAB complies with Quality System Standards ISO 9001 and ISO: 13485) per sample with 2 pg anti-BrdU monoclonal antibody (cat# ab6326, Abcam, Cambridge, UK). Beads were washed four times with 300 pl of PBST wash buffer including SUPERase In RNase Inhibitor (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Plant Biology 2021Quote: ... 2% was removed as input and samples were incubated overnight with 1% antibody (for histone ChIP: anti-H3 (abcam ab1791), anti-H3K27me3 (EMD Millipore 07-449) ...
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bioRxiv - Microbiology 2020Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with mouse monoclonal anti-MBL (Biotin) (2 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam) and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Cell Biology 2020Quote: ... anti-LAMA 1&2 (ab7463, 1:1000), anti-CD59 (ab133707, 1:1000), and anti-GAPDH (ab9485, 1:2500) were purchased from Abcam.
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Primary antibodies were diluted in TBS + 2% BSA + 0.1% Triton X-100 at 5 µg/mL (rabbit anti-OCT4, Abcam #ab19857), 1:200 (mouse anti-NANOG ...
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bioRxiv - Cell Biology 2022Quote: ... The pre-cleared supernatants were divided into two parts and incubated with 40 μL Dynabeads pre-bound with antibodies for 3.5 μg NONO or RNA polymerase II phosphorated at Serine 2 (Abcam, ab5095) and normal mouse IgG overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Developmental Biology 2022Quote: ... Blastocysts were then washed three times in PBS with 2% BSA and then incubated with a secondary anti‐rabbit antibody (1:200, ab150077, Abcam; 1:200, ab150075, Abcam) or anti-mouse antibody (1:200 ...
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bioRxiv - Neuroscience 2022Quote: Slides were similarly processed for BDNF with the exception that slides were blocked with 10% sheep serum in 5% Triton-X PBS under coverwells in a humid chamber for 2 hours and slides were incubated with primary antibodies for BDNF (1:500; ab226843, Abcam) and NeuN (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed three times with PBS and antibody binding was probed using secondary-conjugated Goat antibodies targeting the species of ALG-2 and ALIX antibodies (no. ab175652, ab150113, abcam). Secondary antibodies were diluted 1:500 with 2% BSA in PBS and incubated with cells in the dark for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody incubation was performed for 2 hours at room temperature with slow rocking using Goat Anti-Mouse IgG H&L (ab6789, Abcam). The blots were developed using Clarity Western ECL Substrate (1705061 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... the following antibodies and amounts of antibody were used for the 50,000-cell ChIP: 2 µg of FOXA1 (Abcam, ab170933), 2 µg of H3K4me1 (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used in this study: anti-SARS-CoV-2 spike glycoprotein rabbit polyclonal antibody (dilution 1:1000; #ab272420, Abcam); anti-SARS-CoV-2 nucleocapsid protein rabbit polyclonal antibody (dilution 1:1000 ...