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Citations for abcam :
1501 - 1550 of 3364 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed three times with PBS and antibody binding was probed using secondary-conjugated Goat antibodies targeting the species of ALG-2 and ALIX antibodies (no. ab175652, ab150113, abcam). Secondary antibodies were diluted 1:500 with 2% BSA in PBS and incubated with cells in the dark for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The remaining supernatant was transferred to a 2 mL round-bottom tube and incubated with 5 µg/µl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for one hour ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody incubation was performed for 2 hours at room temperature with slow rocking using Goat Anti-Mouse IgG H&L (ab6789, Abcam). The blots were developed using Clarity Western ECL Substrate (1705061 ...
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bioRxiv - Physiology 2019Quote: Uterine sections were blocked and permeabilized with 5% normal goat serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam) in 5% normal goat serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... in TBST before incubating at room temperature for 2 h with polyclonal or monoclonal antibodies against EGFP (AB290 from ABCAM) or DsRed (AB62341 and AB28664 from ABCAM for cell culture samples ...
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bioRxiv - Bioengineering 2020Quote: ... blocked spheroids were firstly incubated for 2 days at 4 °C with α-Ki67 (Abcam, stock concentration 0.031 mg/mL) or α-cleaved caspase3 (Cell Signalling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated for 2 hrs with a rabbit polyclonal pan-synuclein antibody (1:1000; ab53726; Abcam, Cambridge, MA). After washing in TBST ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS was diluted in the supernatant to 0.1% with FA-lysis buffer and incubated with 2 μg anti-Rpb3 antibody (Abcam 1Y26) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 2% BSA in PBS for 1 hr and subsequently incubated with anti-androgen receptor antibody (1:400, Abcam) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-SQSTM1/p62 (IP 1-2 μg/mg of lysate; ab101266) and rabbit anti-GFP (WB 1:2000, ab290) were from Abcam; mouse anti-SQSTM1/p62 (WB 1:1000 IF 1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were washed 3 times with PB and incubated for 2 hours at room temperature with secondary antibodies (goat anti chicken Alexa Flour 594, abcam, ab150172 ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining lysate was incubated with 5 μl of ssDNA (10mg/ml) and 2 µl of mouse monoclonal anti-V5 antibody (Abcam), 4 µl of rabbit polyclonal anti-PrA (Sigma ...
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bioRxiv - Pathology 2021Quote: ... for 20 min before being incubated overnight with the primary anti-ACE-2 antibody (a polyclonal antibody raised in rabbits and diluted 1:2000, ab15348 Abcam) at 4°C in Tris-Buffered Saline (TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with 1x TBS and incubated for 2 hours at room temperature with appropriate secondary antibodies (goat anti-chicken FITC, 1:200, Abcam or goat anti-rat AF488 ...
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bioRxiv - Physiology 2020Quote: ... in 300 μl of TBS (with 2% goat serum and 0.2% Triton X-100) containing primary anti-desmin antibody (1:50; ab15200, Abcam, UK). After overnight incubation ...
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bioRxiv - Biochemistry 2021Quote: ... Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784, 1:500; PDHAl [9H9AF5], Abcam #ab110330 ...
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bioRxiv - Developmental Biology 2021Quote: ... mice were bred to Rosa26-mTmG (91) and analyzed at 2 months by immunostaining with chicken anti-GFP antibodies (Abcam) and Alexa Fluor 647 hydrazide ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in 2% BSA in PBS to block against non-specific binding and incubated in 2% BSA containing rabbit anti-LC3B primary antibody (Abcam). Coverslips were then washed 3 × 5 minutes in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with 200 μL DMEM and then incubated with a mixture of 2 μL Apopxin Green (Abcam) and 0.4 μL Propidium Iodide (15 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were subsequently subjected to 2 h of incubation with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody (Abcam). Immunoblots were visualized by enhanced chemiluminescence (ECL kit ...
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bioRxiv - Genetics 2020Quote: ... Membranes were probed for 1 hour at room temperature and overnight at 4°C with primary antibody (Anti-8-Hydroxy-2’-deoxyguanosine antibody, 1:1000, Abcam) in 3% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Immunology 2020Quote: ... thawed unfixed tissue slices were rinsed with PBS then blocked with blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) for 1 hour at room temperature before incubation of mAbs at 5 µg/ml overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Laminin alpha-2 was used specifically for costaining and was detected by Alexa Fluor 594 Donkey anti-rat (AB150156; 1:200; Abcam). All sections were mounted in Vectashield (Vector Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... secondary antibodies for another 90 minutes at 37°C and mounted with 4’,6-diamidino-2-phenylindole (DAPI) containing mounting medium (Abcam) as published before14 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed in 1xPBS with 0.05% Triton and incubated for 2 hours at room temperature with a secondary antibody (Abcam, ab150077). Brain slices were washed and mounted in Fluoromount (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... followed by a 2 hours incubation with respective Horseradish peroxidase-coupled secondary antibodies (anti-mouse IgG ab97023 and anti-rabbit IgG ab97051, Abcam). Luminescence was then detected using SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then washed 3X in 0.4% Triton-X-100 TBS for 5 minutes before blocking at room temperature for 2 hours in a solution of 10% Normal Goat Serum (Abcam), 3% BSA (Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Neuroscience 2024Quote: ... groups of wildtype larvae (AB*) at 6 dpf were incubated for 2 hours in 30 mL EM with or without 1 mM cisplatin (Abcam) at 28°C ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...
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Caspase cleavage of Influenza A virus M2 disrupts M2-LC3 interaction and regulates virion productionbioRxiv - Microbiology 2024Quote: Antibodies used in this study were mouse monoclonal anti-IAV Matrix 2 (Abcam, ab5416, WB 1:1000, IF 1:100), rabbit monoclonal anti-LC3B (Novus Biologicals ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were separated on a magnetic rack and washed once before being resuspended in 100 µl antibody buffer (wash buffer, 0.25% Digitonin and 2 mM EDTA) and 1 µl BRG1 antibody (Abcam, ab110641). Beads were incubated with the antibody over night with gentle shaking at 4 °C ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Genomics 2024Quote: ... These strains bearing single-copy integrations were grown overnight at 30°C in rich medium YEA and used for ChIP with 2 µl of anti-GFP (ab290, Abcam). PCR oligonucleotides detecting central core (cc1&3 ...
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bioRxiv - Immunology 2024Quote: ... and were rinsed with PBS for three times before mounting the coverslips to slides in glycerol mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (ab188804, Abcam). The mounted samples were observed on a Zeiss LSM 880 inverted laser scanning confocal microscope ...
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bioRxiv - Cell Biology 2023Quote: ... ab199956), Bcl-2 antibody (1:1000, rabbit anti, ab194583), and gp91phox (1:2500, rabbit anti, ab129068) were purchased from Abcam company ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for chromatin immunoprecipitation: rabbit anti-NK Homeobox 2-1 (NKX2-1, 1 µg per reaction, ab133737, Abcam) and rabbit anti-CEBPA (C/EBPα ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were washed three times with TBST and incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (Abcam) or HRP-conjugated anti-rabbit IgG (Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were blocked with 2% BSA in PBS and then incubated with anti- H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Microbiology 2023Quote: ROS production of the donor strain was measured by using a 2’,7’–dichlorofluorescein diacetate (DCFDA) kit (abcam®, UK), following the manufacturer’s protocol ...