Labshake search
Citations for abcam :
1501 - 1550 of 2293 citations for IL 4 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies targeting Atox1 (Abcam, diluted 1:300). Subsequently ...
-
bioRxiv - Molecular Biology 2023Quote: ... Immunoblots were stained overnight at 4°C with the following primary antibodies: H2A.Z (1:2,000; Abcam ab4174), Secondary goat anti-rabbit (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal aliquots of sonicated chromatin were used per immunoprecipitation reaction with 4 μl H3K27ac antibody (Abcam; 4729) preconjugated to Protein G Dynabeads (Life Technologies) ...
-
bioRxiv - Cell Biology 2023Quote: ... Sections were then incubated overnight at 4°C with goat polyclonal SNAP23 (1.25 µg/ml; Abcam, ab166808), diluted in 1% PBS/BSA ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated overnight at 4 °C in primary antibody (1:100 Abcam Rabbit anti-Atrx ab97508). After primary incubation ...
-
bioRxiv - Systems Biology 2023Quote: ... and then stained overnight at 4°C with a 1:500 Anti-Collagen I antibody (Abcam, ab34710). After overnight incubation ...
-
bioRxiv - Biochemistry 2023Quote: ... membrane extracts were incubated for 1h at 4°C with an anti-NDUFS3 primary antibody (ab14711; abcam) against the matrix arm of complex I from C ...
-
bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated in blocking solution over night at 4 °C (RUNX1 1:200 - ab92336, Abcam). Cells were then washed in PBS-T and incubated with secondary antibodies for 1 hour at room temperature (donkey α-rabbit 1:200 - A-11008 - Thermo Scientific) ...
-
bioRxiv - Genomics 2024Quote: ... Both plates were stained for ALP after 4 days using the Alkaline Phosphatase Staining Kit (Abcam, ab242286) following kit instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated overnight at 4°C with primary antibodies directed Desmin (Abcam #Ab32362; 1:200) in 2% BSA ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4° C overnight in 1:500 recombinant rabbit anti-HA tag (Abcam, AB236632) or rabbit anti-c-Fos (Abcam ...
-
bioRxiv - Immunology 2023Quote: ... Blots were incubated overnight at 4 °C with the following primary antibodies: β-ACTIN (Abcam, 1:5000), Y701 STAT1 (Cell Signaling ...
-
bioRxiv - Developmental Biology 2023Quote: ... and membranes were subsequently incubated overnight at 4°C in blocking buffer with anti-Sox2 antibodies (Abcam, Cambridge ...
-
bioRxiv - Neuroscience 2024Quote: ... Recombinant Anti-α-synuclein aggregate antibody [MJFR-14-6-4-2] - Conformation-Specific (abcam, ab209538, 1:200). Lists of secondary antibodies used ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were then stained with an antibody against enhancer of mRNA decapping 4 (EDC4) (1:200, Abcam) at room temperature for 1 h before washing three times with PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were incubated overnight at 4℃ at the indicated dilutions: goat anti-YFP (1:400, Abcam) and rabbit anti-SPC (1:1000 ...
-
bioRxiv - Bioengineering 2024Quote: ... The following primary antibodies were added overnight at 4°C: anti-H3K27ac (Abcam, #ab177178, dilution 1:7000) and anti-H3K27me3 (Abcam ...
-
bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat. ab108252, Abcam, Cambridge, UK) diluted 1:100 in BSA 1% in PBS without Ca2+ and Mg2+ ...
-
bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
-
bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2019Quote: The detection of specific anti-RSV IgG antibodies in biological fluids was performed using Human Anti-Respiratory syncytial virus IgG ELISA Kit (ab108765, abcam®, Sweden), according to manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... Cells were then washed once with 200 μL PBS containing 1% BSA and incubated with PE conjugated Goat F(ab’)2 Anti-Human IgG-(Fab’)2 secondary antibody (abcam, Cambridge, MA) at a final concentration of 2.5 μg/ml in 100 μL PBS containing 1% BSA per well ...
-
bioRxiv - Immunology 2020Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody (Abcam, ab98532, 1:2000) for 30 min at RT ...
-
bioRxiv - Bioengineering 2020Quote: Albumin secretion was quantified in Liver-Chip effluent collected from the top channel using the Human Albumin SimpleStep ELISA® Kit (Abcam, #ab179887) according to the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Expression of each species’ ACE2 receptor in the cells was confirmed by Western Blot analysis using antibody to human ACE2 (Abcam, Waltham, MA). Parental CRFK cells served as a control (Mock).
-
bioRxiv - Microbiology 2021Quote: ... Chuncheon-si, ROK), anti-HCoV-OC43(LS-C79764, LS-bio, Seattle, WA, USA) and anti-human GAPDH antibody (ab9485, Abcam, Cambridge, UK) overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
-
bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
-
bioRxiv - Cell Biology 2019Quote: The same protocol was used for the staining of VEGF-A expressing cells using a rabbit anti-human VEGF-A monoclonal antibody (ab52917, Abcam, Cambridge, UK), which was revealed using the same secondary antibody as above ...
-
bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
-
bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated with heat-inactivated serum and visualized with a goat anti-human secondary antibody (IgG) conjugated with FITC (Abcam, Cat#ab97224).
-
bioRxiv - Genetics 2020Quote: ... and incubated with rabbit polyclonal anti-PEA-15 (C-terminal amino acids 93-123 of Human PEA15) antibody ab135694 (Abcam, Cambridge, UK) at a concentration of 1:100 and anti-GAPDH antibody (MAB374 ...
-
Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
-
bioRxiv - Molecular Biology 2022Quote: ... blood samples from each repopulated mouse were collected via the saphenous vein and analyzed using the Human Albumin ELISA Quantitation Set (Abcam, Cambridge, UK) according to the manufacture’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for the western blot experiments: mouse monoclonal antibody against human apolipoprotein E (dilution 1:1000; Abcam, Cambridge, UK), and polyclonal rabbit antibodies against the LL37 epitope (diluted 1:1000 ...
-
bioRxiv - Biochemistry 2023Quote: Cell lysates were subjected to SDS/PAGE (0.1% w/v) immunoblotting analysis using an anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... washed three times with PBS and then 200 µl PBS containing anti-human antibodies conjugated to alkaline phosphatase (Abcam, 1:2000 dilution) was added for 1 h ...
-
bioRxiv - Immunology 2024Quote: ... The antibodies were removed and the plates were washed three times with PBS + 0.1% Tween20 before adding anti-human IgG HRP conjugate (Abcam, #ab97225, 1:20000) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and 50 μL Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
-
bioRxiv - Bioengineering 2020Quote: ... The GP-13C6 complex was identified by adding a polyclonal human anti-IgG antibody conjugated to FITC (Abcam, Cambridge, USA; Cat. No. ab97224). Cell cultures were observed under fluorescence illumination with an optical microscope (Axio Imager M2 microscope (Zeiss ...
-
bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 μg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2021Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) for 30 mins at RT ...
-
bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
-
bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...