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Citations for abcam :
1501 - 1550 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and 30ng of this extract was used for subsequent immunoprecipitation using 4μl of antibody targeting H3K4me3 (Abcam AB8580 lot GR273043-3).
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed 3 times in TBST and incubated with the secondary antibody (donkey anti-rabbit IgG HRP conjugate, AbCam 16284) diluted 1/5000 in TBST for 2 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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Varying recombination landscapes between individuals are driven by polymorphic transposable elementsbioRxiv - Genomics 2024Quote: ... We followed the CUT&Tag@home protocol (V.3, (Kaya-Okur et al. 2019; Henikoff et al. 2020)) using H3K9me3 rabbit antibody (Abcam EPR16601) and anti-rabbit Secondary Antibody binding (EpiCypher 13-0047) ...
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bioRxiv - Cancer Biology 2024Quote: ... The assay plate was washed 3 times with 100 µl/well of PBST and 20 µl/well biotinylated detection antibody (Abcam, Ab62352) was diluted (1:100 in dilution buffer ...
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bioRxiv - Genetics 2024Quote: ... washed 3×10 minutes in TBS-T and then blocked for an hour in TBS-T+ (TBS-T with 3% normal goat serum (Abcam, ab7481)) at room temperature ...
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bioRxiv - Genomics 2023Quote: ... primary antibodies (CTCF: D31H2 CST 1:600, MRE11: ab214 Abcam 1:600, PCNA: ab29 Abcam 1:500, RAD21: ab992 Abcam 1:500, STN1: sc-376450 1:200) were added in a 1% BSA in PBS solution overnight at 4°C or RT for 3 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242; P2X7R, 1:50, Abcam/ab109054; S100β, 1:200, Abcam/ab52642; NeuN, 1:200, Millipore/MAB377) overnight at +4°C ...
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bioRxiv - Cell Biology 2021Quote: ... ALP (1:1000, A5111), SHH (1:1000, A5115) (Selleckchem), collagen □ (1:1000, ab255809), OCN (1:1000, ab133612), Runx2 (1:1000, ab54868) (abcam, UK), Ptch1 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... EEA1 (1:200, CST 3288T), Lamin A/C (1:200, CST 4777T), Clathrin (1:200, CST, Synaptophysin (1:200, Abcam ab32127), Synaptotagmin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were procured from the following sources: β-catenin (1:1000, ab16051), CD31 (1:1000, ab222783), podoplanin (1:500, ab10288) and Beclin1 (1:2000, ab62557) from Abcam. Has2 (sc-514737 ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000; EpiGentek A4032) (LAMP1 1:1000; Abcam ab32356) diluted in 5% BSA 0.5% Tween20 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Claudin-1 (Cldn-1) (1:200 prepared in 2.5% goat serum, Cat. No. ab15098; Abcam) for overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... samples were stained using primary antibodies: anti-Nkx2-1 (TTF-1) (1:500, ab76013, Abcam) and secondary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-ionized calcium binding adaptor molecule 1 (Iba-1) (1:50; Abcam, Cat #: ab5076), mouse anti-α-syn phosphorylation at serine position 129 (p129 ...
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bioRxiv - Neuroscience 2020Quote: ... NBQX disodium salt (NBQX, 5 μM) and CGP 55845 hydrochloride (CGP, 4 μM) were obtained from Abcam. Fluorocitrate was prepared as previously described (Paulsen et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μg of lysate was incubated with 5 μg of either polyclonal rabbit anti-SBDS (Abcam, ab128946) or polyclonal rabbit anti-RPS19 (Bethyl-A304-002A ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were diluted 5-fold and glycogen content was analyzed via the Glycogen Assay Kit (ABCAM, ab65620) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... T- REx-293-EV cells were treated with (+)-JQ1 (5 µM, Abcam, Cat# ab141498, dissolved in DMSO) or the equivalent amount of DMSO [0.025% (v/v)] 30 min before TNF-α stimulation ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA and probed with primary antibodies overnight (anti-THEM6, ab121743, anti-CALR, ab22683, Abcam, Cambridge ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were incubated with the anti-p53 antibody PAb 240 (5 μg/ml; Abcam, Cambridge, UK), overnight at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were washed 5× in PBS-T before developing with TMB ELISA Substrate (High Sensitivity) (Abcam, ab171523). Plates were allowed to develop for 3 min before the reaction was stopped by adding 1 N HCl ...
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bioRxiv - Bioengineering 2020Quote: ... serial cross-sections (5 µm) of the aortic root were stained with ORO (Abcam, Cambridge, United Kingdom), H&E ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 6.0 (121°C for 5 min) and stained overnight at 4°C for Esrrg (abcam, ab49129), Gp2 (MBL ...
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bioRxiv - Genetics 2020Quote: ... 2 μg of purified mRNAs were mixed with 5 μg of anti-m A antibody (Abcam, ab151230) in 450 μL immunoprecipitation buffer (50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Physiology 2021Quote: ... washed 2 × 5 min in PBS and then incubated with primary antibodies against Laminin-α2 (#11576, Abcam) in antibody solution containing 10% goat serum in PBS ...
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bioRxiv - Physiology 2020Quote: ... permeabilised and blocked in 5% horse serum prior to incubation in primary antibodies (Collagen type II [Abcam ab34712] 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Ubiquitin (Santa Cruz, Catalog#: sc-9133, IP 10 μl=2 μg; Abcam, Catalog#: ab7780, IP 5 μl), NBS1 (Novus Biologicals ...
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bioRxiv - Bioengineering 2020Quote: ... 5 μg of Antibody (Rabbit Non-Immune IgG, Millipore #12-370, or Rabbit anti-Nrip1, Abcam #Ab42126) was added to the lysates and they were incubated overnight at 4 °C with end over end mixing ...
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bioRxiv - Cell Biology 2023Quote: Frozen tissue sections were washed with 1X PBS and blocked with 5% Normal Goat Serum (Abcam; ab7481) for 2h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl of inactivated sample or type I IFN standard (Abcam, 500, 50 or 5 pg/ µl) was added to HEK-BlueTM IFNα/β cells (InvivoGen hkb-ifnab ...
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bioRxiv - Biophysics 2024Quote: ... Next DMEM was replaced with fresh DMEM which was supplemented with 5 μM of fluo-4AM (Abcam). Plates were then left in an incubator for a further 40 min to enable the cellular uptake of the Ca2+ sensitive fluorescent dye ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% BSA and incubated with primary antibodies for HMOX1 (Abcam Cat# ab52947, RRID:AB_880536), ACO1 (Santa Cruz Biotechnology Cat# sc-166022 ...
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bioRxiv - Molecular Biology 2023Quote: ... Tissue 5-HT levels were assessed using the Serotonin ELISA Kit according to manufacturer’s instruction (Abcam ab133053).
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473; H3K9me2 Abcam ab1220 ...
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bioRxiv - Biochemistry 2022Quote: ... incubated at 95 °C for 5 min and analyzed by SDS-PAGE and subsequent InstantBlue Coomassie (Abcam) staining.
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bioRxiv - Genomics 2022Quote: ... was washed with PBS solution and incubated with 5 μg of mouse monoclonal anti-Ago2 (Abcam, ab57113), anti-CD9 antibody (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... then further mixed with 5 µL antibody (anti-H3K27me3, CST) or 2 µL antibody (anti-H3K27ac, Abcam) and incubated overnight at 4°C on rotator ...
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bioRxiv - Cell Biology 2023Quote: ... small molecule inhibitors and controls were incorporated for final concentrations of: 5 μM Latrunculin B (Abcam, ab144291); 50 μM CK-666 (Calbiochem/Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 μm sections were dipped into xylene and graded concentrations of ethanol as described (http://www.abcam.com/protocols/). Slides were boiled in sodium citrate for 20 minutes ...
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Decreased Astrocytic CCL5 by MiR-324-5p Ameliorates Ischemic Stroke Injury via CCR5/ERK/CREB PathwaybioRxiv - Neuroscience 2024Quote: ... The PVDF membranes were blocked with 5% BSA and probed with primary antibodies (phospho-ERK1/2, Abcam, ab76299 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue sections of 5-μm thickness were cut and immunostained with ELANE antibody (ab68672, Abcam, Cambridge, UK). To quantify the expression of ELANE in the brain ...
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bioRxiv - Cancer Biology 2024Quote: Cells/Organoids were treated with the following compounds as indicated: 5FU ( Abcam, ab142387-5g, 2-5 µM), Metformin (Cayman Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 min at RT and blocked with blocking buffer (5% normal donkey serum (NDS) (Abcam, ab7475) + 1% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... anti-insulin (ab7842, 1:400), anti-glucagon (ab10988, 1:400), anti-somatostatin (ab30788, 1:100), and anti-Ki67 (ab15580, 1:1000) (Abcam, Cambridge, UK). The next day ...
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bioRxiv - Neuroscience 2021Quote: ... Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS, 1:50, Abcam; goat anti-DCX ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1, 1:400, Abcam; rabbit anti-iNOS ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled for amyloid precursor protein (APP) (Chemicon, 1:1000), MAC3 (Pharmingen, 1:400), glial fibrillary acidic protein (GFAP) (Novo Castra, 1:200) or IBA1 (abcam, 1:1000). Images were acquired at 40x magnification using a bright-field light microscope (Zeiss AxioImager Z1 ...