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Citations for abcam :
1451 - 1500 of 1618 citations for Recombinant Enhanced Green Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the cells were then incubated with the following primary antibodies in 0.1% BSA solution (1X PBS) overnight in the dark at 4 °C: mouse monoclonal (1:1000) Respiratory Syncytial Virus (F-protein) (Abcam), rabbit monoclonal (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with the polyclonal rabbit anti- myelin basic protein (MBP) antibody (1:200, ab40390, Abcam) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to PVDF membranes and then immunoblotted with the following antibodies: cleaved Caspase-3 (1:500, Abcam, ab13847), WAVE1 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Molecular Biology 2022Quote: ... The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211) because detection via our anti-DnaD antibody (Eurogentec ...
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bioRxiv - Synthetic Biology 2024Quote: ... Percent purity of each sample was determined by running diluted aliquots of each purified protein on a 4-12% Bis-Tris gel and staining with Optiblot Blue (Abcam). After destaining ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-voltage-dependent anion-selective channel protein 1 (VDAC1)/porin (mouse monoclonal, #ab14734, RRID: AB_443084, Abcam; 1:2000 for WB), anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The co-immunoprecipitated and immunoprecipitated proteins were then characterized by Western Blotting using anti-HA monoclonal antibody (ab18181, Abcam, USA) and anti-myc monoclonal antibody (ab18185 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and size of the protein has been evaluated by Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Pathology 2023Quote: ... Five micrograms of extracted protein was used for western blot analysis with primary antibody against MMP2 (1:200; ab92536, Abcam). The signal intensity of bands was quantified using the Image J densitometry software (NIH).
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Systems Biology 2023Quote: ... The protein expression and solubility were evaluated by both Coomassie stain and western blot (WB) of the GST and MBP fusion proteins using anti-GST (abcam) and anti-MBP (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... pT320-pp1 (1:1000 for the detection of the endogenous protein, 1:30000 for the kinase assay, rabbit, Abcam ab62334), pY15-Cdk1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Concentrations of plasma LPS-binding protein (LBP) were examined via Mouse LBP SimpleStep ELISA according to the manufacturer’s protocol (Abcam, #ab269542). Plasma samples were prepared at a 1:16,000 dilution ...
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bioRxiv - Genetics 2023Quote: ... and Atg18 knockdown flies with the expression of mCherry-Atg8a fusion protein were counterstained with diluted CytoPainter Phalloidin-iFlor 488 (from Abcam) for F- actin at room temperature for 15 minutes before the tissues were mounted in phosphate-buffered saline solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Cell Biology 2024Quote: ... The gels were run in MOPS buffer at 200 V for 45 min and stained with Instant- Blue Coomassie protein stain (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Neuroscience 2024Quote: ... 5mg per sample of lysate was added to 50ul Protein G beads (Cytiva 28951379) pre-bound with 10ul anti-PCB (Abcam 110314 ...
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bioRxiv - Molecular Biology 2024Quote: Protein-nucleosome binding assays were performed by incubating the abovementioned purified Pioneer-seq nucleosome library with human TP53α (Abcam ab84768), TAp63α (Origene TP710041) ...
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bioRxiv - Cell Biology 2024Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Our antibody against DARPP-32 binds to a 16 AA segment on a region of the protein that does not contain any phosphorylation sites (Abcam ab40801) and therefore it labels DARPP-32 irrespective of phosphorylation state and cellular location ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4 °C with Protein G magnetic beads (Dynabeads: ThemoFisher) that had been pre-incubated with anti-H3K27Ac (Abcam, ab4729) or anti-Sox2 (R&D ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Genetics 2020Quote: ... Reactions were stopped by addition of 6x SDS Laemmli buffer and proteins were resolved by 15% SDS-PAGE and analysed by western blotting with anti-ubiquitin antibodies (1:2000 Abcam P4D1) and anti-Rad51 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The signal intensity from the examined proteins were evaluated on free-floating sections using the standard method of simultaneously combined staining (Abcam Protocol). Brain slices (50 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... were resolved by SDS-PAGE and the target proteins were detected with Western blot (WB) using the antibodies as follows: GAPDH Rabbit monoclonal antibody (ab181602, Abcam, UK), SLC40A1 Rabbit polyclonal antibody (ab58695 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were blocked for 1 hour at room temperature with 5% fat-free milk or milk-free blocking buffer (for analysis of phosphorylated proteins) and incubated overnight at 4°C with primary antibodies for p-GCN2 (Abcam, ab75836), p-eIF2α (Cell Signaling ...