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Citations for abcam :
1451 - 1500 of 3978 citations for 6 methyl 4 oxo 5 6 dihydro 4H thieno 2 3 b thiopyran 2 sulfonyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... lamin A/C (1:100 in PBS; following blocking with NGS, 2 hr at 37°C) (Abcam; ab108595, USA), or nucleostemin (1:250 in PBS ...
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bioRxiv - Genetics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG were incubated in lysis buffer and 0.5% BSA for 2 hrs with either rabbit anti-GFP (Abcam) for query IPs or 5 μl of rabbit-anti HA (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with Alexa Flour 488-conjugated goat anti-rabbit antibody (abcam, 1:500 in 2% BSA) and DAPI (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were resuspended at 1 × 106 cells/mL in cell-culture media supplemented with 2 mM thymidine (Abcam ab143719) and incubated at 37 °C in 5% CO2 for 18 hours ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: Floating sections were blocked with 10% goat serum and incubated with chicken anti-MAP-2 (1:250, Abcam, USA) and rabbit anti- Cav-1 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam) according to kit protocol and analyzing the dilution of the dye at 24-hour periods over 3-days and measuring fluorescence intensity ...
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bioRxiv - Neuroscience 2021Quote: ... or the DCS Supervision 2 Polymer System (Innovative Diagnostik-Systeme, PD000POL-K) and the DAB substrate kit (Abcam, ab64238). In situ hybridization for Protocadherin beta 2 (Pcdhb2 ...
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bioRxiv - Cell Biology 2020Quote: ... Blocking buffer was aspirated and cells incubated for 1 h at RT with a 1:1,000 dilution of primary antibodies in 2% BSA blocking buffer against dsDNA (Abcam, Cambridge ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Acta2 (1:100) and anti-nuclear transcription factor Gli family zinc finger (Gli)2 (1:100; ab26056, Abcam) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... blocked with 5% BSA and 0.2% triton X-100 for 2 h and incubated with primary antibody (rabbit anti-pMLKL, 1:200 Abcam) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated for 2 hours with secondary antibody Alexa Fluor 488 donkey anti-rabbit (1:1000 abcam ab150073), and glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2019Quote: ... E-Cadherin (Cdh1) IHC staining was done by using mouse monoclonal [HECD-2] to E-Cadherin (Abcam, cat. ab1416) with M.O.M ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed after 2 hour with 3.5% PFA and proceeded with IF staining with anti-BrdU antibody (ab6326, Abcam) followed with the secondary antibody conjugated with Alexa Fluor 594.
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bioRxiv - Neuroscience 2019Quote: ... The 2 ml of whole blood were added to 40 ml of red blood cell lysis buffer (Abcam, ab204733) and incubated for 10 min at room temperature to lyse red blood cells ...
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bioRxiv - Biochemistry 2021Quote: ... primary antibodies (OAT, Invitrogen #PA5-66715, 1:500; Fumarase, Invitrogen #PA5-82899, 1:500; Casein Kinase 2 beta, Abcam #ab151784 ...
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bioRxiv - Cell Biology 2021Quote: ... A monoclonal antibody that detects serine or threonine linked O-Linked N-Acetylglucosamine-RL-2 (ab2739) was from abcam. Anti-OGT (ab177941) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with either 0.2% saponin or 0.2% triton (see Supporting Table 2) in PBS for 15 m and then blocked with 10% goat serum (Abcam) in PBS for 30 minutes (adding 0.2% saponin for saponin-permeabilized samples) ...
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bioRxiv - Immunology 2021Quote: ... incubated with HRP-conjugated goat anti-human IgG (Fab’)2 (1:5,000 in 1% nonfat dry milk-TBST; Abcam) for 1 h at RT and visualized with KwikQuant Ultra digital ECL substrate using the KwikQuant digital imager (Kindle Biosciences ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: AAV-injected mice were intraperitoneally injected with saline or clozapine-N oxide (CNO) (2 mg/kg, Abcam, Cambridge, UK), returned to their homecage ...
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bioRxiv - Pathology 2022Quote: ... Anti-SARS-CoV-2 was conjugated to PE / R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit / Abcam, ab102918), while anti-CD31 was linked to Alexa Fluor 488 (Abcam ...
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bioRxiv - Physiology 2022Quote: ... The homogenates were centrifuged at 14’000 g for 2 min and the supernatant was used to measure triglyceride using a triglyceride quantification kit (Abcam) by following the manufacture’s instruction ...
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bioRxiv - Physiology 2023Quote: ... in DPBS for 1 h at room temperature followed by incubation in blocking solution (2% donkey serum (#ab7475, abcam), 3% BSA (#A7030 ...
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bioRxiv - Microbiology 2023Quote: ... 2 x 106 parasites were collected and incubated with 200ul FITC conjugated VSG2WT antisera (FITC conjugation kit, Abcam ab102884) (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... pUb (pS65-Ub, Cell Signalling Technologies, 62802S, 1:250) and/or ref(2)P/p62 (Abcam, ab178440, 1:1000), diluted in 1% BSA in 0.3% Triton X-100 PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rehydrated and washed with ddH2O for 2 minutes and mounted using Mounting Medium with DAPI (Abcam, USA), and edges sealed with clear enamel once the mounting medium set.
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bioRxiv - Neuroscience 2023Quote: ... 100□μl of supernatant was saved as “Input.” The remaining supernatant was transferred to a 2-ml round-bottom tube and incubated with 5Lμg/μl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Intrinsic contractility was increased by treating 90-100% confluent Caco-2 cell monolayers with 100nM/mL calyculinA (Abcam #ab141784) for 10-15 min at 37°C.
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bioRxiv - Molecular Biology 2022Quote: ... Staining was performed using antibodies against 8-Oxoguanine (8-OG or 8-hydroxy-2’-deoxyguanosine; Abcam, Cambridge, UK, ab206461), F4/80 (Santa Cruz Biotechnology Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with PBS and lysed with ice-cold lysis buffer containing NP-40 (2%, ab142227, Abcam), Triton X-100 (2%) ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used for western blotting are as follows: rabbit α-LRRK2 (MJFF2 c41-2, ab133474, Abcam, 1:300); rabbit α-phospho-Ser935 LRRK2 (ab133450 ...
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bioRxiv - Microbiology 2023Quote: ... ~10% of each sample by volume was set aside as input and 2 μL of antibody HA (Abcam ab9110) was added to the remaining sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Genomics 2023Quote: ... 100Lμl of supernatant was saved as “Input.” The remaining supernatant was transferred to a 2-ml round-bottom tube and incubated with 5Lμg/μl of anti-EGFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Prior to DENV-2 infection cells were pre-treated with 1 µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: mRNA was isolated as described above and 1 μg was precipitated with 2 μl anti-m6A antibody (ab151230, Abcam) or normal rabbit IgG (sc-2027 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in either recombinant primary rabbit anti-GFAP antibody (astrocytes, 1:500 in 2% BSA and 0.1% Triton [TBS]; Abcam), or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2024Quote: ... or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1) antibody (microglia, 1:1000 in 2% BSA and 0.1% Triton [TBS]; Abcam) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked with 2% BSA and incubated with primary rabbit anti-XBP1 antibody (ab37152, 1:200, Abcam, Cambridge, MA, USA), ATF6 antibody (ab203119 ...
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bioRxiv - Cell Biology 2020Quote: IMR90 cells induced to senescence by oncogenic RAS were prepared and analyzed through ChIP-Seq using anti-histone H3 tri methyl K79 (Abcam; Cat.# ab208189). ChIP-Seq libraries were created using Kapa HyperPrep Kit (Roche Sequencing and Life Science) ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were incubated in blocking buffer containing (TBST+5% BSA) for 1hour at RT and incubated with primary antibody in blocking buffer at 4°C overnight: POLD1 (Abcam Cat# ab168827,1:500); GAPDH (Santa Cruz Biotechnology Cat# sc-32233 ...
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bioRxiv - Genomics 2019Quote: ... permeabilized with 0.1% Triton X100 in PBS for 5 min and incubated overnight at 4°C with anti-α-tubulin (ab7291 from abcam; 1:1000) in PBS containing 0.1% Triton and 0.5 mg/ml BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated either at room temperature for 2 hours or overnight at 4°C with primary antibodies in 5% milk in 1X TTBS at the following concentrations: DNAJB6 (Abcam #ab198995; 1:1000), CTCF (Cell Signaling Technology #2899 ...
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bioRxiv - Cell Biology 2021Quote: Splenic B-cells were pretreated or not with 1 µM staurosporine (Abcam) for 24 h at 37°C in DMEM-BSA to induce apoptosis (Diaz et al ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used include monoclonal mouse anti-cyclophilin-B (CL3901, Abcam, UK), monoclonal rabbit anti-VDR (D2K6W ...
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bioRxiv - Pathology 2022Quote: ... Human kidney biopsies were stained with rabbit anti-phospho-STAT5 B (Abcam) and revealed using Histofine reagent (Nichirei Biosciences).
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TNFR1/p38αMAPK signaling in Nex+ supraspinal neurons regulates sex-specific chronic neuropathic painbioRxiv - Neuroscience 2023Quote: ... phosphorylated nuclear factor kappa B (phospho-NFκB, rabbit, 1:1000, Abcam ab76302), p38 mitogen-activated protein kinase (p38MAPK ...
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bioRxiv - Immunology 2022Quote: ... residual phagocytosis was subsequently blocked with 10 µg/ml cytochalasin B (Abcam) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... Isolated naïve and GC B cells labelled with anti-TFAM (primary, Abcam) and Alexa Fluor 647 conjugated goat anti-rabbit secondary antibody (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-KIF5B rabbit monoclonal antibody clone EPR10276(B) (1:500 abcam ab167429); Anti-Nesprin1 rabbit monoclonal antibody clone EPR14196 (1:250 abcam ab192234) ...
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bioRxiv - Physiology 2022Quote: ... The membranes were incubated with primary antibody against 3-nitrotyrosines (3-NT, Abcam) (1:1000 dilution in 5% FSG TBS-T ...