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Citations for abcam :
101 - 150 of 3762 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... CTCF was immunoprecipitated using protein A agarose (MilliporeSigma) and 2 µg of anti-CTCF antibody (Abcam, ab188408) or rabbit IgG isotype (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ChIP Antibodies (H3K4me1 abcam ab8895, H3K27Ac abcam ab4729, H3K36me abcam ab9050, 2 µg antibody per 2×107 cells) were incubated with 25 µL protein A Dynabeads (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were resuspended at 1 × 106 cells/mL in cell-culture media supplemented with 2 mM thymidine (Abcam ab143719) and incubated at 37 °C in 5% CO2 for 18 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated with goat anti-influenza A virus polyclonal antibody (ab20841, Abcam, 1:10,000 dilution) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... sections were incubated with a primary anti-vaccinia virus antibody (1:10.000, Abcam, ab35219, Berlin, Germany), followed by incubation with secondary anti-rabbit IgG-horseradish peroxidase conjugates (ZytoChem HRP Kit ...
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bioRxiv - Cell Biology 2020Quote: ... Western blot analysis was performed using 100 μg of total protein using anti-ACE2 (Abcam/ab239924/EPR4435(2)) ...
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bioRxiv - Cancer Biology 2020Quote: ... pancreatic or lung CAFs were treated with an inhibitor to beta-site Amyloid precursor protein cleaving enzyme 1 and 2 (BACE) (0.7μM; Abcam) for the duration of CAF CMed generation to inhibit Amyloid β production by the cells.
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bioRxiv - Cell Biology 2021Quote: ... lysed using 62.5 mM Tris buffer (pH 6.8) containing 2% SDS and used for protein quantification using BCA assay (Abcam). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal, Citrate Buffer HIER, dilution 1:2500, Abcam, Code Number: ab242384) was assessed within the brainstem and cerebellum ...
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bioRxiv - Developmental Biology 2022Quote: ... 20 µl of Dynabeads Protein A (for rabbit polyclonal antibodies) and 2 µg anti H3K79me2 antibody (Abcam #ab3594) were combined and rotated overnight at 8 RPM and 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The immunohistochemistry technique was performed with a commercial antibody anti-SARS-CoV-2 spike protein (Abcam, Cambridge, UK) and the in-house mAb 1F7 ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were obtained by lysis of cells in 1xRIPA buffer (Abcam) containing complete Mini ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and B cell lymphoma (Bcl)-2 (Cat#Ab182858, Abcam, Cambridge, UK). Images of chemiluminescent bands were acquired using a Bio-Rad Gel documentation system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... dead cells were labeled with 2 µM of the impermeable cell membrane dye DRAQ7™ (Abcam). In experiments with fixation ...
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bioRxiv - Cell Biology 2019Quote: ... RUNX-2 positive cells were similarly stained using a mouse anti-human RUNX-2 monoclonal antibody (ab76956, Abcam, Cambridge, UK), which was revealed using an Alexa Fluor®488 goat anti-mouse IgG2a secondary antibody (A-21131 ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatant (1 mg protein) was incubated overnight with rotation at 4°C with either nesprin-2 antibody (Abcam, #ab217057) or anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Immunology 2020Quote: ... Virus replication was detected with mouse mAb RSV5H5 anti-RSV M2-1 (ab94805, Abcam, Cambridge, MA, USA) followed by addition of HRP-conjugated goat anti-mouse IgG second antibody (catalog number 1030-05 ...
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bioRxiv - Cancer Biology 2020Quote: ... histone proteins were isolated from cells by using histone extraction kit (Abcam - ab113476) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Histone proteins were extracted from T cells using a Histone Extraction Kit (Abcam) following manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... Cells were loaded with leak-resistant Ca2+ indicator Fura-2 AM (Abcam) and measured using the IonOptix imaging system and software (IonOptix ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam) for 15 min at 4 °C to visualize the cell nuclei ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-CAV1 (for use with Caco-2 BBe1 cells; Abcam ab2910), and mouse anti-GAPDH (AM4300 ...
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bioRxiv - Cancer Biology 2020Quote: ... ERK1/2 (Cell Signaling Technology, #9102, 1:1000; Abcam #ab17942, 1:1000), p-MEK (S217/221 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were exposed to actinomycin D (2 µg/mL; Abcam, USA) for different time intervals ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were incubated for 10 min with Pitstop 2 (30 µM; Abcam)24 ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...
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bioRxiv - Cell Biology 2020Quote: ... We prepared bead-antibody complexes by 4 h incubation of protein A/G agarose beads with 2 μg of antibody (anti-histone H3 ab1791; anti-histone H3K4me3 ab8580; both Abcam) in total volume of 500 μL of dilution buffer followed by O/N blocking of antibody-bead complexes ...
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bioRxiv - Neuroscience 2022Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were grown and protein expressed in 2xYT media induced with the addition of 1 mM IPTG and 2 mM nhpSer (DL-AP4, Abcam) at 37 °C for 18 h.
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with human TruStain FcX and staining with anti-influenza A virus nucleoprotein antibody (431; Abcam) in 0.1% Triton X-100 solution ...
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bioRxiv - Microbiology 2020Quote: FLAG-tagged neuraminidase proteins were immunoprecipitated from cell lysates per the manufacturer’s instruction (Abcam). Briefly ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with EV71 VP1 protein specific mouse monoclonal antibody (clone 10F0; Abcam), and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L ...
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bioRxiv - Genomics 2021Quote: ... CD8+ T-cells or purified c-Myc human recombinant protein (Abcam, ab169901 Cambridge, UK) as previously described (19 ...
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bioRxiv - Immunology 2020Quote: ... T cell plasma membranes were purified with Plasma Membrane Protein Extraction Kit (ab65400; Abcam) and absence APC telomeres assessed by confocal imaging on Leica SP2 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonyl residues in were quantified in 10 μg of the cell lysates using the Protein Carbonyl Assay Kit (Abcam, Boston, MA, USA, ab178020). Derivatization and neutralization were carried out according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated with 10 µM of pitstop 2 (Abcam, Cambridge, MA, USA) or 0.5-2.0 µg/mL ikarugamycin (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... the C3H/10T1/2 cells were labelled with CFSE (10 μM, Abcam, ab113853) staining and seeded on 100 mm culture dishes ...
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bioRxiv - Genomics 2019Quote: ... 1-2 million sorted cells were stained with anti-lamin B1 (Abcam, ab16048) antibody at a concentration of 0.5μl/million cells ...
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bioRxiv - Genomics 2020Quote: ... Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (ab104139, Abcam). After sealing with neutral gum ...
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bioRxiv - Cell Biology 2021Quote: ... The overexpression of WT and mutant c-Myc protein in cells were confirmed by immunofluorescence staining of transfected cells with anti-c-Myc antibody (Abcam). The sequences of cloning primers were listed in Table S4.
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Immunology 2021Quote: ... Proliferation of cells was measured staining of control and Irradiated UVC with either 2 μM Cell Trace Yellow (Abcam) according to kit protocol and analyzing the dilution of the dye at 24-hour periods over 3-days and measuring fluorescence intensity ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then probed for 1 h with a polyclonal rabbit anti-SARS-CoV-2-S protein antibody (1:2,250; ab252690; Abcam, Cambridge, UK) or a rabbit anti-MeV N protein antibody (1:1,000 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...