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Citations for abcam :
101 - 150 of 159 citations for Cyanine2 NHS ester minimal dye since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... After adding 2 µL of DNA dye (Hoechst 33342, Abcam, Cat # ab228551) 150μL of nuclei solution (Hoechst only) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with Fluo-8 AM calcium dye (5 µM, Abcam) for 15 min at room temperature in Tyrode solution (130 mM NaCl ...
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bioRxiv - Genetics 2024Quote: ... cells were incubated with the MitoRed dye-working solution (Abcam, Cambridge, UK) for 30 minutes in a 37°C 5% CO2 incubator ...
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bioRxiv - Genomics 2024Quote: ... Nuclei were visualized by staining the tissue with DRAQ5 dye (abcam ab108410) at a 1:300 dilution in 1X PBS for 30-60 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with Hoechst 33258 Staining Dye Solution (Abcam, cat. #ab228550) for 45 min at 4°C in darkness ...
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bioRxiv - Neuroscience 2020Quote: ... cells were shortly permeabilized in 1x PBS with 0.1%Triton-X-100 and incubated for 1h30 in 10% NHS with chicken anti-GFP (1:2000, Abcam) and rabbit anti-PlexinA4 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Biochemistry 2020Quote: ... The membrane was immunoblotted with primary antibodies: mouse anti-strep (1:2500, Fisher NBP243719) and rabbit anti-thiophosphate ester (1:2000, Abcam ab133473), washed ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was vacuum dried and the dried lipids were dissolved in 200 μL of the assay buffer supplied by the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, ab65359). 5 μL of each sample was used for each reaction.
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bioRxiv - Biochemistry 2023Quote: ... The lipid fraction from 1 million cells per sample was extracted after which total cholesterol was assayed using the Cholesterol/Cholesteryl Ester Quantitation Assay kit (Abcam, ab65359) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: The levels of total and free cholesterol released in medium were measured using a colorimetric cholesterol/cholesteryl ester detection kit (Abcam, ab102515) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a cell permeable analog of 1,2-bis-(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid acetoxymethyl ester (BAPTA-AM, Abcam, 10 μM) (Tsien ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.1% 4-Iodophenylboronic acid (4IBPA) and 0.2 % 5-TAMRA fluorescent dye (Abcam, ab145438) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then treated with dye-labeled secondary antibody (ab175660, abcam, UK) solution in PBS and incubated for 1h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... were incubated with the cell tracking dye Cytopainter Green (ab138891, Abcam, 1:1000) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 μL hydrogel ...
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bioRxiv - Cancer Biology 2023Quote: The cell fluorescent labelling was performed using CytoLabeling reagent dyes (Abcam, Cambridge, UK) according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2023Quote: ... secondary antibodies and dyes include: anti-GFP (1:1000, Abcam ab13970, RRID: AB_300798), anti-ZO-1 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated with 3 µM Cal-520AM dye (ab171868, Abcam) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2, ab18465, Abcam; 1:1000 Rabbit anti-GABAalpha6 ...
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bioRxiv - Genomics 2023Quote: The cholesterol assay was carried out according to the manufacturer protocol using the Cholesterol/Cholesterol Ester Assay Kit (ab65359, Abcam, Cambridge, UK) and normalized to total protein content as determined by Bradford Assay.
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bioRxiv - Microbiology 2022Quote: ... Mitochondrial membrane potential was measured using a tetramethylrhodamine ethyl ester (TMRE) and carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) as a control (Abcam, Cambridge, UK). Prior to incubation and acquisition ...
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bioRxiv - Developmental Biology 2024Quote: ... were incubated with the cell tracking dye Cytopainter Green (1:500, cat# ab138891, Abcam) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 uL hydrogel ...
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bioRxiv - Cancer Biology 2023Quote: ... 1×105 cells were incubated with H2-DCFDA (non-fluorescent dye, Abcam, Boston, MA)/ CellROX Orange (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies and dyes (E-cadherin BD Bioscience #610181, dilution 1:100; CDX2 [EPR2764Y] Abcam ab76541 ...
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bioRxiv - Cell Biology 2022Quote: The MMP (ΔΨm) of cells was evaluated using the cationic fluorescent dye JC-1 (Abcam). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... following which sections were blocked in 3% normal horse serum (NHS) for 30min before overnight incubation with Tyrosine hydroxylase (TH) (Abcam, ab112, 1:750) or IBA-1 (Wako ...
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bioRxiv - Developmental Biology 2024Quote: Serum vitamin D (25(OH)D) levels of mouse serum samples were assessed by commercially available ELISA kits (Eagle Biosciences, Inc. Amherst NH, or Abcam, Cambridge, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... We utilized the following primary antibodies and dyes: rat anti-BrdU antibodies (1:800, Abcam, ab6326), rabbit anti-RELB (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with secondary antibody (anti-mouse CW800) and DNA dye Draq5 (Abcam, Cambridge, UK), diluted 1:10000 in blocking buffer and incubated for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated either with phalloidin conjugated to Alexa 488 dye (Phalloidin-iFluor 488, Abcam ab176753) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... glucose 13.8 mM) and loaded with a cell permeable fluorescent calcium sensitive dye Fluo8-AM (Abcam) at 5 μM and allowed for 30 min incubation at 37°C ...
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bioRxiv - Immunology 2021Quote: Real-time viability of T cells in different culture conditions was determined using DRAQ7 dye (Abcam, ab109202), which selectively fluorescently stains dead cells ...
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bioRxiv - Biophysics 2021Quote: ... 12 hours prior to harvesting 1 µmol total/well DRAQ7™ dye (Abcam, Cambridge, United Kingdom. ab109202) was added to each well [53 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells pre-incubated with NO dye and assay buffer 1 (Fluorometric Nitric Oxide Assay Kit, Abcam, UK) for 30mins at 37°C in 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: The fluorescent probe JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) cationic dye (Abcam, Cat# Ab141387) was used to analyse the mitochondrial membrane potential (Ψm) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed and mixed with non-heat-treated cells and the viability dye DRAQ7 (Abcam, UK) was added to a final concentration of 3 μM prior to loading as a mixed population ...
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bioRxiv - Neuroscience 2024Quote: Extracellular oxygen consumption was measured using an oxygen-sensitive fluorescent dye (Extracellular Oxygen Consumption Assay Kit, Abcam) as per the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... then switched to either normo-glucose or glucose-deprived media containing the viability dye DRAQ7 (Abcam ab109202, 1:100). Immediately following the media change ...
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Perivascular Excitation Tunnelling: a Novel and Preventable Cause of Cardiac Reperfusion ArrhythmiasbioRxiv - Physiology 2023Quote: ... Hearts were loaded with voltage-sensitive dye (25 µL bolus of 8.6 mM di-4-ANBDQPQ, Cytocybernetics, Buffalo, NY, USA) and electromechanically uncoupled ((±)blebbistatin ([Abcam, Amsterdam ...
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bioRxiv - Immunology 2024Quote: ... The cells were stained with a secondary anti-mouse antibody conjugated with Alexa Fluor 647 dye (1:1,000, #ab150115, Abcam) for 1 h at RT in the dark ...
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bioRxiv - Developmental Biology 2024Quote: ... the membranes were incubated with secondary antibodies conjugated with fluorescence dyes (anti-mouse IRDye® 800CW, 1:5000, Abcam, ab216774 ...
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bioRxiv - Microbiology 2020Quote: ... and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam). Cells were washed 3 × (MBL2 wash buffer ...
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bioRxiv - Bioengineering 2020Quote: ... hCMEC/D3 and primary rodent microglia cells were labeled with Cell Tracking Dye Kit - Green - Cytopainter (ab138891, Abcam, Cambridge, UK) and Cell Tracking Dye Kit - Deep Red - Cytopainter (ab138894 ...
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bioRxiv - Neuroscience 2023Quote: ... were labeled with the fluorescent dye Alexa Fluor 647 according to the manufacturer’s protocol (Alexa Fluor 647 conjugation kit lightning link, Abcam, ab269823). Briefly ...
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bioRxiv - Microbiology 2022Quote: Membrane fluidity was measured as previously described (136) using a membrane fluidity kit that quantifies the fluorescence of a lipophilic dye (Pyrenedecanoic acid) (Abcam). Pyrene decanoic acid exists in monomeric and dimeric states within membranes ...
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bioRxiv - Cell Biology 2024Quote: ... To allow visualization of interaction during live cell imaging T cells were stained with the Calcium-sensitive dye Cal520 (Abcam) prior to injection ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µl of 1 mM DCFDA dye in 1X buffer and 5 µl of 10 µM DiOC2 was added for ROS evaluation (ab113851, Abcam) and membrane depolarization (B34950 ...
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bioRxiv - Cancer Biology 2021Quote: ... transduced cells were cultured at 37 °C for 60min in the presence of the Hoechst 33342 live-cell staining dye (Abcam, USA) to a final concentration of 10μM ...