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Citations for abcam :
1401 - 1450 of 1466 citations for L Tyrosine Ring 13C6 99%; 3 3 D2 30% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Bioengineering 2024Quote: ... the slides were incubated with secondary antibody Goat Anti Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077, 1:500 dilution) for 30 min at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies used were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (1:500; Cat# ab150077, Abcam, UK) and Goat anti-Rabbit IgG (H+L) ...
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bioRxiv - Cell Biology 2024Quote: ... and then washed 3X with PBST and incubated for 1 hour in secondary antibody Goat Anti-Mouse IGG H&L Alexa Fluor® 488 (ab150113, Abcam, 1 ...
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bioRxiv - Cancer Biology 2024Quote: MKN7/HGC-27 cells that overexpressed or have knockdown of IGF2BP3 were collected and processed according to the kit instructions (Glucose Assay kit, Abcam, ab65333; L-Lactate Assay kit, Abcam, ab65331; ATP Assay Kit, Abcam, ab83355). In brief ...
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bioRxiv - Cell Biology 2024Quote: The activity of Cathepsin L from isolated cardiomyocytes from 6-8 mice (plated as described) was assessed using the Cathepsin L Activity Assay kit from Abcam (ab65306). 50μg of protein from isolated cardiomyocytes were used to assess the degradation of the FR-AFC substrate at 37°C degrees for 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% NP-40, in TBST) for 30 min at room temperature and then incubated with anti-GFP (1:2000, #ab290) (Abcam, Cambridge, United Kingdom) anti-Zld (1:750 ...
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bioRxiv - Cell Biology 2021Quote: ... CD63 staining was performed by incubating with mouse anti-CD63 (TS63 Diaclone 857.770.000 1/200) in PBS-BSA 1% for 30 min and CD9 staining was performed incubating with rabbit anti-CD9 (Abcam ab236630 1/80) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were then permeabilized with 0.2% Triton X for 30 minutes at room temperature followed by blocking with 5% normal donkey serum (Abcam plc, Cambridge, MA, USA) and 300 mmol/L glycine for one hour at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Slides were immunostained overnight at 4°C or 4h at 30°Cwith a mixture of the two primary antisera diluted in blocking buffer [rat anti-tubulin (Abcam ab6160 1:1250) and rabbit anti-MYC (Santa Cruz ...
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bioRxiv - Developmental Biology 2020Quote: ... After the incubation with the primary antibodies the embryos and pupal dissections were washed three times for 30 minutes in PBT and incubated with the respective secondary antibodies: Alexa Fluor 488 conjugated Donkey Anti-Goat (abcam Cat. No: ab150129), Alexa Fluor 488 conjugated Goat Anti-Rabbit (ThermoFisher Scientific Cat ...
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bioRxiv - Cell Biology 2021Quote: ... blocked in goat serum for 30 min and incubated overnight at 4°C with primary antibodies against DOT1L (Abcam, ab64077, 6 µg/ml), HIF-1α (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... the slides were blocked with 10 % goat serum containing 1 % BSA at room temperature for 30 minutes and then incubated with the primary antibody against EGFP (Abcam, ab184601, 1:100) overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... for 30-45 seconds. Then counter stained with Harris Modified Hematoxylin (Epredia Cat. No. 72704) or Meyers Modified Hematoxylin (Abcam Cat. No. ab220365) for 10-30 seconds ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) labeled with Alexa Fluor®-647 (Alexa Fluor® 647 Conjugation Kit (Fast)-Lightning-Link® (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then permeabilized and blocked with the blocking buffer [0.1% Triton X-100 and 2% bovine serum albumin in phosphate buffered saline (PBS)] for 30 min and subsequently incubated with the primary antibodies rabbit anti-αTubulin (Abcam, ab18251,1:1000 dilution), mouse anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-nucleocapsid (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at room temperature (RT) for 30 min protected from light in the presence of anti-spike antibody (Abcam, clone 1A9, ab273433) or anti-H3 (A/Perth/16/2009 ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked with 10% goat serum plus 1% BSA at room temperature for 30 minutes and then incubated with the primary antibodies against EGFP (Abcam, ab184601, 1:100) mixed with Neurofilament-L (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2019Quote: ... cells were resuspended at a concentration of 2×106 cells/ml in serum-free media containing 30 μg/ml of an IgG mouse isotype control antibody (abcam, Cambridge, MA, Catalog #ab91353) or a function blocking anti-β1 integrin antibody (abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 30 min at room temperature and blocked in 10 % serum (goat [Thermo Fisher Scientific, Catalog No. 16210064] or donkey [Abcam, Catalog No. ab7475]), 0.01% Triton X100 and 0.3M glycine in PBS for 4h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed twice with 200 μL of cold DPBS/BSA and incubated for 30 min on ice with 50 μL of 1:500 dilution of anti-beta subunit cholera toxin (Abcam, catalog no. ab 34992). For antibody staining ...
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bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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bioRxiv - Bioengineering 2022Quote: ... and incubated for 1h at room temperature with anti-mouse or anti-rabbit IgG H&L secondary antibodies conjugated with Alexa Fluor™ 488 or Alexa Fluor™ 568 (goat, 1:500; Abcam). Slides were mounted with VECTASHIELD antifade mounting medium containing DAPI (Vector Labs ...
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bioRxiv - Biophysics 2022Quote: ... The cells were washed with PBS three times before labeling with goat anti-rabbit IgG H&L Biotin (ab97049; abcam, Cambridge, UK) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were washed 3x with PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed 3x with 100 μL PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at room temperature and a secondary antibody (1:500; Donkey anti-mouse IgG H&L, Alexa Fluor® 568, Abcam #ab175472) for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... anti-occludin antibody (ab216327, and goat anti-rabbit IgG H&L (Alexa Fluor 488, ab150077) antibody were all obtained from Abcam (Waltham, MA). Anti-tumor necrosis factor-α (aTNF ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA); Ro 3306 ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: The following secondary antibodies have been used for Western blotting: Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Abcam, #ab7068; 1:10000), Recombinant Protein G (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... and secondary antibody solutions were added for 1 hour at room temperature: donkey anti-rabbit IgG H&L (Alexa Fluor 647) at 1:200 (Abcam, Cambridge, Massachusetts); donkey anti-mouse IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2023Quote: A pair of parotid salivary glands were harvested from each mouse (n=5/group) and directly placed in 500 uL of Lactate Assay Buffer from the L-Lactate Assay Kit (ab65331, Abcam, Cambridge, UK), 10 uL of protease inhibitor (PI ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were washed three times with 1X PBS and then incubated with the secondary antibody (Goat Anti-Rabbit IgG H&L, Alexa Fluor® 647, Abcam, ab150079) at a 1:500 dilution in 2% BSA in 1X PBS for 1 hour at room temperature in the dark ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Biophysics 2021Quote: ... the cells were blocked for 1 h at room temperature using an unconjugated goat anti-mouse IgG H&L antibody (polyclonal, 1:1000, Abcam ab6708, Cambridge, UK) diluted in previously mentioned blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed 3 times and then stained simultaneously with two secondary antibodies: goat anti-mouse IgG H&L (Alexa Fluor® 488) preadsorbed (1:400 diluition; ab150117; Abcam, Cambridge, UK) and rhodamine red -X-coniugated affinity pure goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody was added to each tube (10μL anti-Histone H3, Cell Signaling Technology, cat #4620; 4μg Normal Rabbit IgG, Cell Signaling Technology, cat #2729; 4μg anti-SNF2H, Abcam, ab72499). Two SNF2H IP replicates were performed ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by a 48h incubation in goat antirabbit IgG H&L (Alexa Fluor 555) antibody (1:500, Abcam, Abcam Cat# ab150078, RRID:AB_2722519) and then placed for 1 h in a Hoescht:PBS (1:10 000 ...
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bioRxiv - Microbiology 2024Quote: ... Antigen-specific IgG antibodies in hamster sera were detected using horseradish peroxidase (HRP)-labeled goat anti-Syrian hamster IgG H+L Ab (Abcam, 1/30000 dilution). IgG1 and IgG2/3 subclass Abs were analyzed using biotinylated subclass-specific antibodies (Southern Biotech ...