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Citations for abcam :
1401 - 1450 of 1487 citations for 6 Chloro 2 iodotoluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The samples were blocked with 5 % (v/v) FBS in PBS for 30 min and incubated with a rabbit polyclonal anti-COX-2 primary antibody (ab15191, Abcam, Cambridge, UK) diluted at 1:100 in 1 % (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was removed using a magnetic Eppendorf tube rack prior to adding 2 µg of anti-citrulline antibody (ACAb, Abcam, Cambridge, UK) diluted in 200 µL phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2019Quote: ... GluN receptors were blocked by adding 4 μl of 25 mM d,l-2-amino-5-phosphonovaleric acid (APV; Abcam Cat# ab144498) to the 1 ml of aCSF in the interface recording chamber ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained for intracellular spike protein using an Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam, ab275759, 1:50) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor 488 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Immunology 2020Quote: ... Followed by capture of SARS-CoV-2 receptor binding domain which was biotinylated using the lightning-link type-A biotinylation kit(Expedeon/Abcam, 370-0005) for 180s at 10ul/min ...
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bioRxiv - Immunology 2022Quote: ... or CD8+ cells using a rabbit-raised anti-SARS-CoV-2 S protein antibody (1:5000 dilution, ab272504, Abcam plc, Cambridge UK), a rabbit monoclonal anti-CD4 antibody (1:250 dilution ...
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bioRxiv - Microbiology 2022Quote: ... paraffin-embedded sections were firstly subjected to immunostaining with a commercially available and diluted (1:5,000) rabbit-anti-SARS-CoV-2 spike (S) protein (ab272504, Abcam Plc., Cambridge, UK), followed by staining with a peroxidase-conjugated secondary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were blocked for 35 min by incubation with solution of 1% of BSA in PBS and stained with 2 μg/mL Rabbit Anti-EEA1 antibody - Early Endosome Marker (ab2900, Abcam, Cambridge, UK) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... 100 ug of anti-SARS-CoV-2/S2 (1A9) antibody was conjugated to A647 using the Lightning-Link Conjugation Kit (Abcam, Cat# ab269823) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight at 4 ℃ in an antibody diluent (1.1% Triton-X and 2% normal goat serum in PBS) containing primary antibodies anti-TUBB3 (mouse, 1:1000, Sigma-Aldrich, T8660; rabbit, 1:300, Abcam, ab18207), anti-SOX2 (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were incubated overnight at 4°C with rabbit monoclonal anti-syntaxin 2 antibody (7.8 µg/ml; Abcam, Cambridge, England, UK: ab170852) or goat polyclonal anti- SNAP23 antibody (1.25 µg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415; YTHDF1 antibody, Abcam ab220162) and incubated at room temperature for one hour ...
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bioRxiv - Microbiology 2022Quote: Immunohistochemistry was performed on formalin-fixed cryosections of mouse aortic roots using antibodies to identify macrophages (MOMA-2, 1:50; ab33451, Abcam, Cambridge, MA), followed by detection with biotinylated secondary antibodies (1:400 ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... H3Cit staining of fixed surface labelled cells was performed by incubating the cells overnight at 4°C in PBS 2% HI-FBS + 2mM EDTA + 0.1% saponin with monoclonal anti-H3Cit antibody (Abcam, ab219407, 1:600). Cells were washed and then incubated with donkey anti-rabbit AF555 (Invitrogen ...
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Locomotion-dependent auditory gating to the parietal cortex mediates flexible multisensory decisionsbioRxiv - Animal Behavior and Cognition 2024Quote: ... The samples were incubated at 4℃ for 48 hours with mouse anti-CaMKIIα antibodies (ab22609, abcam, 1:250 dilution in 2% NGS). After treating the primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were then incubated overnight at 4°C with the following primary antibodies: anti-COX-2 (Abcam, UK, 1:1000 dilution), anti-IL-1β (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with goat-anti-mouse IgG secondary antibody conjugated with horseradish peroxidase (ab6789, Abcam, Cambridge, UK; 2 mg/ml) at a final concentration of 0.8 μg/ml / in 1:2500 dilution in milk blocking buffer for 1.5 hours at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... listed in Supplemental Table 2 were used to quantify TrxB expression levels with the GFP ELISA kit (Abcam, Cambridge, UK, Cat# ab171581) according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... membranes were washed three times and incubated at 4°C overnight in 2% skim milk solution (1g skim milk powder, 50mL TBST) with the primary antibody (GFAP; #ab7260, Abcam, Cambridge, UK) and housekeeper protein (GAPDH ...
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bioRxiv - Cell Biology 2020Quote: ... the primary rabbit mono-specific antibodies were diluted into a concentration of 2-4 µg/ml in blocking buffer (PBS + 4% FBS) containing 1 µg/ml mouse anti-alpha-tubulin (Abcam, ab7291, Cambridge, UK) and chicken anti-KDEL respectively (Abcam ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies were applied for 1-2 hours at room temperature and are as follows: Goat α-Rabbit-HRP (Abcam, ab97051, 1:5000), Donkey α-Rabbit-HRP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... we used the Maxpar Antibody Labeling Kit (Fluidigm) according to manufacturer’s instructions for addition of the respective metal tags: Axin2 (145-Nd, Abcam, ab32197, 2 µg/ml), p-MEK1/2 [S217/221] (151-Eu ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin (125 µg/IP) was incubated overnight in 2 µg of either rabbit polyclonal anti-histone 3 lysine 4 trimethyl (H3K4me3; Abcam, Cat# ab8580, RRID:AB_306649) or anti-Histone 3 (Abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Bioengineering 2022Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was removed into a LoBind tube carefully so as not to remove any beads and 2 µg of anti-GFP antibody (Abcam ab290, anti-rabbit) were added to the sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... e cells were then incubated with primary antibodies against mouse 8-hydroxy-2’-deoxyguanosine (8-OHdG) (1:200)(ab48508, 1:200 dilution; Abcam, Cambridge, United Kingdom) at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... brains were incubated in primary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Chicken anti-GFP (Abcam ab13970; 1:1000); anti-TH mouse monoclonal (SIGMA MAB318 ...
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunostaining was performed with the following antibodies at 1:50–500 dilutions in blocking solution for 1–2 hours at room temperature: anti-DDDD-K (Abcam, Cambridge, UK, ab1162), anti-GFP (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Trisbuffered saline (TBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Rabbit polyclonal HPA antibodies targeting the proteins of interest were dissolved to 2-4 μg/ml in blocking buffer (PBS + 4% FBS) containing 1 μg/ml mouse anti-tubulin (Abcam, ab7291, Cambridge, UK). After washing with PBS ...
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bioRxiv - Physiology 2019Quote: Cyst sections were blocked and permeabilized with 3% normal donkey serum in PBS with 0.3% Triton X-100 for 2 h and incubated overnight with rabbit anti-beta-III tubulin antibody (ab18207, 1:2000, Abcam, Cambridge, United Kingdom) in 3% normal donkey serum in PBS with 0.3% Triton X-100 at 4°C ...
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bioRxiv - Physiology 2020Quote: ... diluted in PBTD was added for 2 hours followed by an anti-elastin rabbit polyclonal antibody diluted in PBTD 1:100 (Abcam, Cambridge, UK; ab21610). The samples were incubated with the primary antibody for 18 hours at 4°C followed by 30 minutes washing in PBTD ...
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bioRxiv - Cell Biology 2021Quote: ... ∼ 5×1010 EVs collected from the two steps of purification were diluted into 500 μl of PBS and 2 μg of rabbit monoclonal anti-CD9 (Abcam, Cambridge, U.K., ab92726) or rabbit IgG (Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Physiology 2021Quote: ... 4µm sections of paraffin-embedded mouse kidneys were submitted to antigen retrieval for 20 min at 95°C in citrate buffer followed by incubation with Ly-6B.2 antibody (Abcam, ab53457, 1:100). Sections were incubated with HRP-labeled secondary antibody (Vector ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Media was changed 2 h post-infection and the cells were fixed and immunostained 48 h post-infection to detect firefly luciferase (Abcam #ab181640, RRID # AB_2889835).
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...