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Citations for abcam :
1351 - 1400 of 2938 citations for Rat CCL11 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Membranes were then washed with TBST buffer (5 × 1 min) and probed with secondary rabbit anti-rat antibodies conjugated with peroxidase (1:1000, Abcam #ab6734). Blots were washed as described above and detection was carried out using Amersham ECL Western Blotting Select Detection Reagent (GE Lifesciences #RPN2235) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by washing in PBS (3 × 15 min) and an overnight incubation (>16 hr at RT) with the primary antibody (1:1000, rat anti-Ctip2, Abcam #ab18465) diluted in antibody solution (1% goat serum and 0.2% Triton X-100 in PBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... blocked with 10% BSA for 1 hour and incubated at 4°C overnight with rat anti-BrdU antibody (1:500, Abcam, ab6326) and rabbit anti-Pax6 in 1% BSA in PBT ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400 ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-rat Fis1 monoclonal antibody (1:1000, Abcam, USA), rabbit anti-rat Cyto c monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam; 1/1000 guinea pig anti-DCX AB2253 Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-glucagon (ABCAM; ab82270) primary antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were incubated for 2.5h with primary Abs for (IdU: 1:100, mouse monoclonal anti-BrdU, Becton Dickinson 347580; CldU: 1:100, rat monoclonal anti-BrdU, Abcam 6326) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-GFP (1:1000, Nacalai Tesque, #04404-84, RRID: AB_10013361) and rabbit anti-GFP (1:5000, Abcam, #ab6556, RRID: AB_305564). The following day ...
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bioRxiv - Microbiology 2020Quote: ... 3 colonic and 1 intestinal tissue fragments of each mouse were incubated with a primary polyclonal 1:200 rat anti-E-cadherin (#ab11512; Abcam USA) in PBS–3% BSA for overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Rat Monoclonal anti-Myosin (MAC 147) (cross-reacts with Myosin in Drosophila; Immunofluorescence: 1:100) was obtained from Abcam (Cat. Ab51098). Rabbit anti-GFP (TP401 ...
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bioRxiv - Genetics 2022Quote: ... Samples were incubated with primary rat anti-Mp antibodies (1/100) (Harpaz et al., 2013) or goat anti-GFP (1/500) (Abcam ab5450) overnight at 4 °C followed by 3 washes with PBS-Tween 0.1% ...
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bioRxiv - Neuroscience 2022Quote: ... The rats received bilateral lesions in the LHb with ibotenic acid (5 µg/µl in 0.01 M PBS, Abcam, Cambridge, UK). The coordinates for the LHb (AP −3.3 mm ...
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bioRxiv - Neuroscience 2022Quote: ... and their respective secondary antibodies (Alexa Fluor 594 anti-rabbit IgG, ThermoFisher; A11037 and Alexa Fluor 488 goat anti-rat, Abcam; ab150157). Sections were mounted and coverslipped with Vectashield Hardset Antifade Mounting Medium with DAPI (Vectorlabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then incubated overnight at 4° C with both α-Cytokeratin-8/18 (1/10, rat monoclonal, University of Iowa, US) and α-Myc antibody (1/200 [Y69], ab32072, rabbit monoclonal, Abcam). After washing with PBS-T ...
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bioRxiv - Physiology 2024Quote: ... followed by secondary antibody incubation (1:200 dilution, goat anti-rat IgG H&L, Alexa Fluor® 488, Abcam, ab 150157,). The density of Mac-2 positive macrophages in the RV was determined by the total cell count in at least 5 random visual fields of the mid RV free wall CSA divided by the total (tissue ...
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bioRxiv - Biochemistry 2023Quote: Rat sera were incubated at 4°C for 16 h in the presence of anti-adiponectin antibodies from Abcam (ab 56416) and Cell Signaling (2789s) ...
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bioRxiv - Bioengineering 2023Quote: ... tissue sections were first washed with 1x PBS and then incubated with secondary antibodies of donkey anti-rat Alexa Fluor 405 (1:500, Abcam, ab175670), donkey anti-rabbit Alexa Fluor 488 (1:500 ...
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bioRxiv - Pathology 2023Quote: ... The rat monoclonal anti-mouse dipeptidyl peptidase-4 (Dpp4 also known as CD26) and the rat monoclonal anti-mouse F4/80 antibodies were purchased from Abcam (ab42899) and AbD serotec (CL:A3-1) ...
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bioRxiv - Cancer Biology 2024Quote: ... the slides were blocked in PBS-BSA 1% + 0.001 % Tween-20 for 1h at RT and incubated with a mix of rat monoclonal anti-BrdU antibody (1:500, Abcam Ab6325) and mouse monoclonal anti-BrdU antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... CTIP2 (chicken ovalbumin upstream promoter transcription factor-interacting protein) antibody was added (rat, 1:500, Abcam, Cat: ab18465, Lot: GR3272266-13) to facilitate delineation of striatum and incubation continued until the 4 hours were completed ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% BSA and 1% normal goat serum overnight at 4°C with primary antibodies: rat anti-Ctip2 (1/500; Abcam, #ab18465), rabbit anti-Cux1 (1/500 ...
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bioRxiv - Developmental Biology 2022Quote: ... the Catalase kit buffer (Abcam, kit ab83464) for catalase activity ...
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bioRxiv - Cell Biology 2019Quote: ... or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2019Quote: ... Visualized neurons were labeled for chicken ovalbumin upstream promoter transcription-factor interacting protein-2 (CTIP2, 1:1000, rat, Abcam, Cat#:ab14865, RRID:AB_2064130) and pre-proenkephalin (PPE ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-ADMA (5,6, kind gift of Pr C. Haass, Munich Germany, 1/100) or rabbit anti-Ubi (Abcam, ab179434, 1/100). After 3 rinses in PBS ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006, Donkey anti Rabbit 647 Abcam ab150075,). Slides were again washed 3 times 5 minutes each with PBS-T ...
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bioRxiv - Physiology 2023Quote: ... Adjacent sections were used for immunohistochemistry with anti-VDR (1:100, rat) and anti-AMH (1:50, mouse) monoclonal antibodies (Abcam, Cambridge, UK) as per manufacturers’ protocol with modifications.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice with PBST before being incubated with the primary antibody (Mouse anti-BrdU, 1:1000, Cat. No. B35128, Invitrogen; or Rat anti-BrdU, 1:1000, Cat. No. ab6326, Abcam, RRID: AB_305426) and the secondary antibody (Donkey anti-mouse 488 or 568 (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies (AF488 goat anti-mouse IgG, Abcam catalog #ab150113; AF594 goat anti-rabbit, Abcam catalog #ab150080; AF680 goat anti-rat IgG, Abcam catalog #ab175778) were diluted 1:500 in DPBS with BSA and Triton-X100 and incubated for 75 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% Triton X for one hour before being staining in in PBS with 0.3% Triton X with rat anti-BrdU (Abcam: 6326 at 1:200), and mouse anti-NeuN (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed twice with PBS and resuspended in goat anti-rat IgG Alexa Flour 647 secondary antibody (Abcam, Cambridge, United Kingdom) diluted 1:1000 in Blocking Buffer and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... SAA and perilipin were performed using a rat anti-mouse F4/80+ antibody and rabbit anti-mouse perilipin (both 1:100 dilution, Abcam®, Cambridge, UK), and a rabbit anti-mouse SAA (1:200 dilution ...
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bioRxiv - Physiology 2020Quote: ... for 1 h followed by a 72 h incubation with primary antibodies as appropriate: rabbit anti-IBA1 at 1:1000 (Wako, 019-19741, Madison, WI) and rat anti-TMEM119 at 1:250 (abcam 209064, Cambridge, UK) [24 ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibodies rat monoclonal anti-CD13 (Novus, NB100-64843, 1:500) and rabbit monoclonal anti-Sox9 (Abcam, Cat. No. ab185966, 1:500) were diluted in Tx buffer (0.2% gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... and the last sample was added with 1 μL of anti-c-Myc rat IgG1 (1 mg/mL; clone JAC6, Abcam #ab10910, RRID: AB_297569). The antibody binding was performed for 2–3 h at 4°C on a rotator ...
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bioRxiv - Genetics 2019Quote: ... Slides were immunostained overnight at 4°C or 4h at 30°Cwith a mixture of the two primary antisera diluted in blocking buffer [rat anti-tubulin (Abcam ab6160 1:1250) and rabbit anti-MYC (Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: MKN7/HGC-27 cells that overexpressed or have knockdown of IGF2BP3 were collected and processed according to the kit instructions (Glucose Assay kit, Abcam, ab65333; L-Lactate Assay kit, Abcam, ab65331; ATP Assay Kit, Abcam, ab83355). In brief ...