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Citations for abcam :
1351 - 1400 of 1420 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Immunology 2024Quote: ... sections were repaired using EDTA antigen recovery buffer and incubated overnight at 4 °C with anti-dengue virus 1+2+3+4 primary antibody (Abcam, ab26837). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... washed twice with 3 % BSA and incubated for 1 h with monoclonal rabbit anti-Ki-67 antibody (Abcam, ab16667, 1:1000) and washed three times with PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Slices containing VPMpc were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... The sections were incubated overnight with anti-cell marker cluster of differentiation (CD) 3 antibody (1:200, Abcam, ab16669, Cambridge, UK) at 4°C in a humidified incubation chamber ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in PBS with 3% donkey serum and the following primary antibodies for 72 h at 4°C: goat anti-Iba1 (1:500; Abcam, ab5076), rabbit anti-ACSL1 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3 to 7 days on X-tra® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546 ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS for 5 minutes and incubated with AlexaFluor® 647 conjugated secondary antibodies (Ab150079, Ab150115; Abcam) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked in TBS containing 3% BSA for 1h at RT and incubated with rabbit anti-B7H3 (1:1000, clone EPNCIR122, Abcam, ab134161) and rabbit anti-GAPDH (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: 10 µl of the supernatant obtained after the first centrifugation at 18000g from the Glycogen assay where combined with 90 µl of Lactate assay Buffer (Abcam® L- Lactate Assay kit, #ab65331) and separated in two wells of a 96 well plate for sample and sample background control ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min at room temperature with primary antibody dilutions prepared in 3 % BSA in PBS as follows: mouse anti-Bub1 (ab54893, Abcam, 1:500); mouse anti-Mad2 (sc-65492 ...
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bioRxiv - Pathology 2019Quote: ... Soluble proteins (20 μg) were used for Western blotting with primary antibodies against AKT1/2/3 (rabbit monoclonal antibody; EPR16798 Abcam; 1:10,000), mTOR (rabbit monoclonal antibody ...
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bioRxiv - Bioengineering 2020Quote: ... Permeabilized cells were rinsed 3 times with 1X PBS and incubated in 1X PBS with primary antibody Rabbit-α-Tomm20 (Abcam, ab186735) and Mouse-α-LaminA/C (Abcam ...
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bioRxiv - Physiology 2020Quote: ... Cells were washed 3 × in TBS and incubated overnight (4°C) in 300 μl of anti-desmin (Abcam Cat# ab15200, RRID: AB_301744) primary antibody made up in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated for 3 h at room temperature with the goat anti-chicken antibody conjugated with Alexa Fluor® 488 (Abcam 150169) at a concentration of 1:500 in PBS-blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in PBS + 1% Tween® 20 + 5% BSA for 1 h at room temperature then incubated overnight with specific primary antibodies at 4°C.Membranes were washed in PBST (3×5 min) then incubated with HRP-conjugated goat anti-rabbit (ab6721) or rabbit anti-mouse (ab6728) secondary antibodies (Abcam, Cambridge, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures subsequently underwent 3 cycles of 10 min PBS washes and incubated for 40 min with the appropriate secondary antibodies (Abcam or Jackson) and DAPI ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies (all diluted in 3% BSA in PBS) were used at the final concentration indicated: chicken anti-GFP (ab13970 from Abcam, 1:5000), rabbit anti-mCherry (GTX128508 from Genetex ...
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bioRxiv - Molecular Biology 2022Quote: ... NAD(P)H quinone dehydrogenase 1 (NQO-1; #ab80588) and pan-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #ab8245) antibodies were provided by Abcam (Cambridge, England). The p38 MAPK inhibitor SB203580 (#S8307 ...
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bioRxiv - Microbiology 2020Quote: ... 1:300 dilution of mouse anti-PfDHODH #3 (a kind gift from Margeret Phillips, University of Texas Southwestern) or 1/5000 mouse anti-PfAldolase-HRP (Abcam, Cat #ab38905). The next day ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
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bioRxiv - Immunology 2022Quote: ... albicans hyphae for 3 hours and then labelled with anti-histone H3 citrulline R2+R8+R17 (Abcam, Cambridge, MA; 0.014 mg/ml) followed by donkey anti-rabbit IgG Cy3 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2019Quote: GAPDH activity was measured in the presence and absence of GAs using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Colorimetric Assay Kit (ab204732, Abcam, Cambridge, MA) as per the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Consecutive sections were processed for immunohistochemical staining with reovirus-specific rabbit polyclonal antiserum (1:1000) or activated caspase-3 (Abcam, 1:100). Images were taken on an EVOS FL Auto 2 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times in PB and were mounted on a glass slide in the mounting medium with DAPI (Abcam ab 104139). The slides were imaged at 20X on a Zeiss LSM 880 airyscan confocal microscope.
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bioRxiv - Physiology 2021Quote: ... Next day the sections were washed 3 × 10 minutes with PBS and stained with an anti-rabbit secondary antibody (1:1000, AB150077, Abcam, Alexa 488) in 2% BSA in PBS for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the sections were washed 3 more times in PBS followed by incubation in the secondary antibody (antirabbit Alexa Fluor-488, Abcam; Sydney, Australia) diluted in blocking solution (1:250 ...
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bioRxiv - Neuroscience 2022Quote: ... samples were blocked in PBTx (1% Triton X-100 in PBS) containing 3% bovine serum albumin and incubated over three nights with chicken anti-GFP antibody (Abcam Cat#ab13790). After several washes in PBTx ...
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bioRxiv - Molecular Biology 2023Quote: ... 100-200 μg of precleared chromatin was incubated at 4°C for three hours with magnetic beads that had been prebound to one of the following antibodies: 3 μg of α-V5 antibody (Abcam ab9116), 1.5 μg of α-H3K9me2 antibody (Abcam ab1220) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were then incubated overnight at 4°C with the following primary antibodies diluted in PBS 3% BSA 0.2% Triton X100: anti-β-Tubulin (clone 2A1A9, dilution 1/200, Abcam, Cambridge, UK), anti-YAP1 (clone D8H1X ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies were diluted in 3% BSA/PBS and incubated overnight at 4°C: rabbit anti-RPA34 (Abcam ab97594, 1:1000), mouse anti-PML (Santa Cruz Biotechnology sc-966 ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices containing locus coeruleus and NTS were incubated in primary antibody (1:500 dilution of 3% normal goat serum in 1% Triton X100 dissolved in PBS, rabbit anti-mCherry IgG, Abcam catalog # ab167453) for 24-48 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed 3 times with 1X PBST before adding anti-Rabbit secondary antibody (Catalog No: ab150080, Abcam, Waltham, MA, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were blocked with 10% goat serum/3% BSA/0.3% Triton X-100 and stained with antibodies to Netrin-1 (Abcam #ab126729, 1:200), Cytokeratin (human specific cytokeratin 7 and 8 ...
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bioRxiv - Cell Biology 2019Quote: The following primary antibodies were used for immunofluorescence imaging (at the indicated final concentration diluted in 3% BSA in PBS): chicken α-GFP (ab13970 from Abcam, 1:5000), guinea pig α-Cenp-C (BT20278 from Caltag + Medsystems) ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin (125 µg/IP) was incubated overnight in 2 µg of either rabbit polyclonal anti-histone 3 lysine 4 trimethyl (H3K4me3; Abcam, Cat# ab8580, RRID:AB_306649) or anti-Histone 3 (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Neuroscience 2022Quote: ... The same procedure was used for flat-mounts but primary incubation was for 3 days and secondary incubation for 1 day using anti-GFP 1:500 (Abcam, Cambridge, UK; Chk).