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Citations for abcam :
1351 - 1392 of 1392 citations for 6 Nitro 2 tetralone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... This was followed by a 2-hour incubation with secondary antibody goat anti-rabbit Alexa Fluor 488 (1:500, #ab150077, Abcam, Waltham, MA, USA), and a 0.1% (w/v ...
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bioRxiv - Physiology 2021Quote: ... 4µm sections of paraffin-embedded mouse kidneys were submitted to antigen retrieval for 20 min at 95°C in citrate buffer followed by incubation with Ly-6B.2 antibody (Abcam, ab53457, 1:100). Sections were incubated with HRP-labeled secondary antibody (Vector ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553). Each experiment was repeated with similar results using anti-SARS-CoV-2 N rabbit polyclonal Ab (GeneTex # GTX135357 ...
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were incubated first with a blocking solution (BSA 1%, NGS 2%, Triton X-100 0.1%) and then with primary antibodies for KIF1A (Abcam, #ab180153, 1:100, rabbit), HTT (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... Media was changed 2 h post-infection and the cells were fixed and immunostained 48 h post-infection to detect firefly luciferase (Abcam #ab181640, RRID # AB_2889835).
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bioRxiv - Molecular Biology 2022Quote: ... blocked with blocking solution at 37°C for 3 h and incubated for 2 h with primary antibody (H3K9ac – Abcam ab12179, 1:300) and 1 hour with secondary antibodies (Donkey anti mouse – Abcam ab150109 ...
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bioRxiv - Genomics 2022Quote: ... The cells were washed three times with PBS and resuspended with 2% BSA-PBS containing a goat anti-rabbit IgG FITC conjugated secondary antibody (Abcam catalog no. ab6717) and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... slices were pre-incubated at room temperature for 2 hours in a blocking medium composed of PB-Tx and Normal Goat Serum (NGS, 5% or 10%, Abcam, Cat. No. ab7481), after which they were incubated with primary antibodies (72 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... midguts were incubated at room temperature for 2 hours on an orbital shaker with secondary antibodies (goat anti-rabbit IgG Alexa Fluor 488 [Abcam] at 1:4000) diluted in midgut blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... DRG or spinal sections were incubated with secondary antibodies for 2 h: goat anti-mouse IgG H&L (Alexa Fluor® 488) (1:500, Abcam, Cat#: ab150113), goat anti-guinea pig IgG H&L (Alexa Fluor® 488 ...
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bioRxiv - Immunology 2023Quote: ... cells that were stained with anti-AMPKα1 antibody and unconjugated isotype control antibody were incubated in the dark at room temperature for 2 hours with anti-rabbit Alexa Fluor 488 secondary antibody (Abcam cat. no. ab150113). Following antibody incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were incubated in blocking solution for 2 hr and then incubated with goat anti-rabbit-HRP secondary antibody (Abcam; ab6721; 1:300) overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary staining was performed for 2 hours at RT in a PBS (1x) solution containing secondary antibody-TRIC labeled (Abcam, MA, 1:1000), BSA (1% ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2023Quote: ... The NOS2 antibody complex was stripped by heating with Bond Epitope Retrieval 2 and then stained with CD8 (Abcam no. 101500, 1:100) or IFNγ (Abcam no ...
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bioRxiv - Cancer Biology 2024Quote: ... The epithelial nature of the primary cultures and single-cell clones were analysed using a recombinant anti-EpCAM antibody (EPR20533-63; Catalogue number: ab221552, Abcam; Supplementary Figure 2).
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bioRxiv - Bioengineering 2019Quote: ... cells were resuspended at a concentration of 2×106 cells/ml in serum-free media containing 30 μg/ml of an IgG mouse isotype control antibody (abcam, Cambridge, MA, Catalog #ab91353) or a function blocking anti-β1 integrin antibody (abcam ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Genomics 2020Quote: ... A 1/20 fraction of the supernatant was used as input sample and the remaining material was split for three IP reactions with an anti-H3K9me3 antibody (Abcam, ab8898, lot: GR148830-2). An amount of 4 μg antibody was added to each IP sample ...
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bioRxiv - Genomics 2022Quote: ... A cocktail of antibodies against the glucocorticoid receptor was added to chromatin input for immunoprecipitation: 2 μg of ab3570 (Abcam, lot GR3222141-5, discontinued) and 0.49 μg of 12041S (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... Blastocysts were then washed three times in PBS with 2% BSA and then incubated with a secondary anti‐rabbit antibody (1:200, ab150077, Abcam; 1:200, ab150075, Abcam) or anti-mouse antibody (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked with 1×TBST containing 5% dry milk for 2-hr at room temperature and probed with the following antibodies: anti-Kv7.4 antibodies (#ab84820, Abcam, Cambridge, United Kingdom; 1:500), anti-REST antibodies (#ab21635 ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% nonfat dry milk at room temperature for 2 hours and then incubated at 4°C overnight with primary antibodies against β-tubulin (Abcam, Cambridge, MA, USA), PD-L1 (ABclonal ...
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bioRxiv - Biochemistry 2022Quote: ... Then cellular expression of ACE2 or syndecan-1 were detected by rabbit monoclonal antibodies (ACE2, Novus bio, NBP2-67692, 1:500, Syndecan-1, abcam, ab128936 2 μg/mL) and secondary donkey anti-rabbit Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in PBST containing 2% NGS with goat anti-rabbit secondary antisera conjugated to Alexa Fluor® 488 (1:1000, Abcam, Cambridge UK, #ab150169) and Alexa Fluor® 647 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Microbiology 2022Quote: ... The higher molecular weight part of the membrane was incubated overnight at 4ºC in anti-DNAK antibody (1:5000 dilution in blocking buffer, Abcam#69617 Mouse monoclonal [8E2/2]) as the loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then blocked in 5 % milk in 1X TBS-T for 1 hour at room temperature followed by overnight incubation in primary antibody against pSer129 (pS) α-Syn (1:2 000, Abcam, 51253, Cambridge, UK), α-Syn (1:2 000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Embryos were then fixed in 4% paraformaldehyde overnight and immunohistochemistry was performed according to previous protocols (Dasgupta et al., 2019) with minor modifications using an anti-acetyl H3K27 – primary antibody (Abcam, AB177178; 2 µg/mL) and a goat-anti-rabbit IgG secondary antibody (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... DR sections (from −5.0 mm to −4.0 mm from bregma in the anterior-posterior axis) were co-stained with primary antibodies against tryptophan hydroxylase-2 (goat α-TPH2, Abcam 121020, at 1:400) as a marker of serotonergic neurons and vesicular glutamate transporter-3 (guinea pig α-VGLUT3 ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescently labelled detection antibodies (AT8: Invitrogen, Cat. No. MN1020, 5 nM; HT7: Invitrogen, Cat. No. MN1000, 2 nM; T181: abcam, Cat. No. ab232849, 5 nM) were added in blocking solution ...
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bioRxiv - Genomics 2023Quote: ... were blocked and pH-calibrated as per the manufacturer’s protocol and 5 μl bed volume of beads were loaded with 2 μg of ChIP antibody (H3K27ac; Abcam-AB4729, H3K115ac; PTM BIO-PTM-170). Beads were added to the solubilized chromatin ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoblotting was next performed by incubating the membranes overnight at 4°C with the following primary antibodies: mouse monoclonal anti-mitofusin 2 antibody [6A8] (abcam, ab56889, dilution of 1/1000), rabbit polyclonal anti-mitofusin 2 (abcam ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1 ...
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bioRxiv - Genetics 2021Quote: ... This was centrifuged and supernatant was collected into a new tube and incubated with anti-H3K27 antibody (ab177178, Abcam, 2 μg for 25 μg of chromatin) or anti-H3K4me1 antibody (ab8895 ...
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bioRxiv - Neuroscience 2023Quote: ... 1% bovine serum albumin in PBS with 0.1% Tween20 [PBST]) for one hour at RT and left overnight in the A2AR primary antibody at 4°C (Abcam #ab3461, Lot # GR3431019-2, 1:100) in blocking solution ...
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bioRxiv - Immunology 2024Quote: ... overnight at 4°C and the relevant secondary for 1h at room temperature (Streptavidin-AF488 Invitrogen S11223 at 2 µg/mL final, anti-mouse IgG1-Cy5 Abcam ab136127 at 1.5 µg/mL final). After nuclear staining with DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then rinsed in PBS three times for 10 min each and incubated for 2 hr in secondary antibodies (goat anti-rabbit Alexa Fluor® 405 for the PV antibody, (abcam, Cat# ab175652, RRID AB_2687498, 1:500), goat anti-mouse Alexa Fluor® 594 for the 8-oxo-dG antibody (abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were prepared using SDS sample buffer followed by Western Blotting and antibody probing procedures according to the guidelines of the company for the respective antibodies (anti-PPARα: H-2, sc-398394, 1:1000, Santa Cruz Biotechnology; anti-CPT1α: ab128568, 1:1000, Abcam; anti-ERRα: ab137489, 1:1000, Abcam). Imaging was done using KODAK films or the Amersham Imager 680 equipment.