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Citations for abcam :
1351 - 1400 of 10000+ citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... in phosphate buffered saline (PBS) prior to overnight incubation at 4°C with blocking buffer containing primary antibodies raised against HIS48 (Abcam, Ab33760, 1:20), CD68 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... neutrophils on coverslips were fixed with 4% paraformaldehyde (PFA) and staining was performed with antibodies specific for H3Cit (citrulline R2 + R8 + R17, Abcam Ab5103, 1:200), myeloperoxidase (MPO ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were blocked with 5% skimmed milk for 2 h at room temperature and then incubated with primary antibodies overnight at 4 °C as follows: anti-collagen I (ab270993; Abcam, USA; 1:1000), anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Cell Biology 2023Quote: ... 1:400 dilution) and anti-α- synuclein aggregate antibody (Clone#: MJFR-14-6-4-2; 1:1000 dilution) were acquired from Abcam (Cambridge UK).
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bioRxiv - Cell Biology 2023Quote: ... Fixed and blocked cover slips were subsequently incubated overnight at 4° C with a rabbit anti-collagen I (Abcam ab260043, 1:250 dilution) primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... for one hour and then stained overnight at 4 °C with primary antibody Chk pAb to GFP (Abcam Cat# ab13970, dilution 1:300). In the next day ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were incubated either at room temperature for 2 hours or overnight at 4°C with primary antibodies in 5% milk in 1X TTBS at the following concentrations: DNAJB6 (Abcam #ab198995; 1:1000), CTCF (Cell Signaling Technology #2899 ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were blocked in 1% BSA in PBS for 30 minutes at room temperature before overnight incubation at 4°C in primary antibodies: α-mouse CENP-A (1:1000 Abcam ab13939 (discontinued) or 1:1500 Enzo ADI-KAM-CC006-E ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated with primary antibodies (ON; 4°C) that were appropriately diluted in blocking solution: chicken anti-GFP (Abcam; ab13970; 1:500), rabbit anti-GFP (Santa Cruz Biotechnology ...
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bioRxiv - Bioengineering 2024Quote: ... Incubation with the primary antibody was carried out at 4°C overnight in a PBS (1x) solution containing anti-Ki67 (Abcam, MA, 1:100), BSA (1%) ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 hour at room temperature followed by overnight incubation at 4°C with primary antibody against TRP2/DCT (1:200, Abcam, catalog number ab74073). Alexa fluor-647 labeled secondary antibody (1:300 ...
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bioRxiv - Neuroscience 2024Quote: ... Tween-20 in tris-buffered saline (TBS-T) then incubated overnight at 4°C with one of the following primary antibodies: rabbit anti-GluK4 (Abcam; ab10101, 1:500), rabbit anti αB-crystallin (Enzo Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... Membranes were blocked with Odyssey blocking buffer (Li-COR) and immunoblotted overnight (4°C) with rodent OXPHOS cocktail monoclonal antibody (Abcam; ab110413, 1:1000) to detect ETC proteins ...
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bioRxiv - Cell Biology 2022Quote: ... milk and the membranes incubated at 4°C overnight with rabbit Anti-Annexin A1/ANXA1 antibody monoclonal antibody [EPR19342]-BSA and Azide free (1: 25000, Abcam, Cat. N. ab222398), ALIX (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Slides were then stained with the primary antibody diluted in 10% BSA in TBS-T overnight at 4 °C: FAP (Abcam, ab207178, 1:250) and POSTN (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then incubated overnight at 4°C with primary antibodies directed against NP and GFP to amplify the Sec61ß-mEmerald signal (Abcam 13970, 1:500) in staining buffer (PBS supplemented with 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated (overnight, at 4°C) with the primary antibody for detecting p-S129 aSyn (rabbit mAb EP1536Y Abcam, #ab51253-1:4000). Then ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were blocked in 5% skim milk diluted in PBS-T for 1 hr at room temperature and incubated overnight at 4°C with a rabbit synaptophysin primary antibody (YE269, Abcam, Cambridge, UK; #ab32127) diluted 1:2,000 in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... at room temperature for 1 h and then incubated under agitation at 4° C overnight in a blocking solution containing the following primary antibodies: Rat anti-BrdU/CldU (Abcam, 1:1000, ab6326), and Mouse anti-IdU monoclonal antibody (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... overnight at 4°C and monoclonal mouse anti-human MUC5AC (Lidell et al., 2008)(1:200) in block solution over night at 4°C (Cat# ab3649, Abcam, Cambridge, UK, RRID:AB_2146844). Secondary antibody was polyclonal donkey anti-rabbit Alexa Fluor 488 (Cat# A-21206 ...
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bioRxiv - Bioengineering 2024Quote: ... in PBST for overnight at 4°C followed by PBS washing and incubation with FITC conjugated secondary antibody Goat Anti-Rabbit (IgG) (Abcam, U.K., 1:2000) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections at the level of the PnC of GlyT2-eGFP mice injected with pAAV DJ-CamKIIα-mCherry in the CeA (N=6 mice) were incubated with a chicken anti-mCherry (1:1000, Abcam, catalog# ab205402, lot# GR225123-3) and a rabbit anti-PSD95 (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed 3-times with PBS and stained with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Abcam, ab150077; RT, 1 h) to detect Aβ ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed 3 times with PBS and stained with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Abcam, ab150077; RT, 1 h) to detect Aβ ...
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bioRxiv - Developmental Biology 2020Quote: ... the samples were fixed with 4% PFA in PBS overnight at 4°C and then blocked by incubation in 10% normal goat serum (Abcam, #ab156046) diluted in 0.1% Triton X-100/PBS (PBT ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal cultures were fixed by incubating cells with a solution of 4% PFA and 4% sucrose for 10 minutes at room temperature before incubation with primary antibody (Abcam, ab6556). In another set of experiments ...
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bioRxiv - Cell Biology 2021Quote: ... or intracellular markers (C-MYC, KLF-4, NANOG, OCT-4 and SOX2) (Santa Cruz, Sc-40; Abcam, ab214666; ab106465; ab18976; ab97959) was performed in the dark at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... then incubated overnight at 4°C with the following primary antibodies in 2% w/v BSA: Rabbit anti-Gclc antibody (Abcam, United Kingdom; 1:1000); rabbit anti-NQO1 antibody (Cell Signalling ...
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bioRxiv - Neuroscience 2020Quote: ... mice were fixed with 4% paraformaldehyde and the brain was sectioned at 200 μm thickness and immunostained against GFP (Abcam, no. ab13970, 1:300). Confocal microscopy (Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2021Quote: ... Pre-synaptic motoneuron terminals were then labeled by incubating bundles overnight at 4°C in the same blocking solution to which had been added a mouse anti-synaptophysin antibody (AB8049 Abcam, USA; 1:25 dilution). The following morning bundles were washed (5 x 30 min ...
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bioRxiv - Physiology 2022Quote: ... 0,05% Tween 20 (TBS-t) for 60 min at RT and incubated overnight at 4°C with anti-IL6 antibody (Abcam, ab229381, 1:1000 dilution). Following 3 washes (10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4◻C in varying mixtures of the following primary antibodies: rabbit anti-DARPP-32 (1:1000; Abcam Cat# ab40801, RRID:AB_731843), mouse anti-Hu (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubations were performed for 24 hours at 4°C using an anti-glial fibrillary acidic protein (GFAP; Abcam, ab53554; 1:250 dilution) antibody ...
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bioRxiv - Microbiology 2020Quote: ... The rest of the chromatin was incubated overnight at 4°C with 1 μg of polyclonal anti-GFP antibody (Abcam ChIP grade, Abcam 290) or ...
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bioRxiv - Pathology 2020Quote: ... Immunostaining was performed by incubating different sections overnight at 4 °C using the following antibodies: human COMP (Abcam Cambridge, MA ab11056-rat 1:100), pS6 (1:200 2215S rabbit polyclonal Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... the fixed tissue sections were reacted individually overnight at 4°C with the following primary antibodies: rabbit anti-CD68 antibody (1:500, Abcam, Cat. #125212, Cambridge, MA), rabbit anti-CD146 antibody (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Neuroscience 2024Quote: ... while the lower half was probed at 4 °C overnight with a 1:1000 dilution of a rabbit anti-β actin antibody (ab8227, Abcam, Waltham, MA, USA), in antibody dilution buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained overnight at 4°C in blocking solution with the following antibodies and dilutions: Alexa Fluor® 488 anti-S100 beta (1:1000, Abcam, #ab196442, clone EP1576Y), PE anti-Nestin (1:150 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2% Tween 20) with 5% milk and incubated overnight at 4 °C with the primary antibodies: rabbit monoclonal anti-histone H2B (Abcam Cat#ab188291, 1:2000) or mouse monoclonal anti-beta actin (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... After blocking 25 µL was removed and 25 µL of primary antibody solution in 1% BSA and 0.3% Triton-X100 in PEM was added to stain the cell body and flagella overnight at 4°C (anti-tubulin, Abcam #ab6161, 1:1000 dilution). Due to the amount of time required for imaging ...
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bioRxiv - Neuroscience 2024Quote: ... before blocking in 10% BSA and incubation with primary anti-bodies against aggregated α-synuclein (MJF-14; rabbit MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), total α-synuclein (mouse LB509 ...
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bioRxiv - Neuroscience 2024Quote: ... fol-lowed by 5% horse serum before incubation with the primary antibody MJF-14 (anti-conformation-spe-cific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:200). The immunohistology sig-nals were visualized with ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (VectorLabs ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS before incubation with the primary antibody MJF-14 (anti-confor-mation-specific α-synuclein filament antibody MJFR-14-6-4-2, Abcam, #ab209538, 1:25,000), overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... after 24 h in culture to a solution of 0.4 mM kainic acid (#ab120100, Abcam, USA) diluted in culture medium for 2 h.
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bioRxiv - Systems Biology 2023Quote: ... and separate sections with Periodic acid Schiff (PAS) to quantify glycogen content (PAS, Abcam, Catalog #ab150680). Images of sections were captured at 10x magnification using an Olympus BX-41 microscope and QImaging QIcam 12-bit Fast 1394 Camera with Bioquant Osteo 2013 Software ...
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bioRxiv - Cancer Biology 2024Quote: ... Periodic Acid Schiff/Alcian Blue (PAS/AB) staining was performed per the manufacturer’s instructions (Abcam, ab245876). For standard IHC on human tissue sections ...
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bioRxiv - Synthetic Biology 2024Quote: Determination of free sialic acid by colorimetric assay was conducted using a commercial kit (abcam, ab83375) following manufacture recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and were then incubated overnight at 4 °C with following primary antibodies diluted in TBST: Anti-alpha Actinin 4 rabbit monoclonal antibody (Abcam, Cat # ab108198), Anti-Vimentin mouse monoclonal antibody (abcam ...