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Citations for abcam :
1301 - 1350 of 2126 citations for Recombinant Rat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and stained with InstantBlue Coomassie protein stain (Abcam, ab119211). The protein-containing fractions were pooled and concentrated in an Amicon spin concentrator of 10-30kDa cutoff (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein carbonyl assay kit (western blot) (Abcam ab178020) was used to assess the oxidation status of mitochondrial proteins according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C in blocking solution with 1% NDS and primary rat anti-BrdU (1:500, Abcam, Cambridge, UK). On the third day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated for 24 hours at 4°C in a primary antibody solution containing rat anti-BrdU (catalogue no. ab6326; Abcam, Cambridge, UK) diluted 1:500 in 0.3% Triton-X and 3% NDS in 0.1M PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with secondary antibody for 1 hr at room temperature: rat-HRP 1:10,000 dilution (abcam ab205720 Lot# GR32115155-1), rabbit-HRP 1:10,000 dilution (Santacruz sc-2004 Lot#B2216) ...
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bioRxiv - Physiology 2022Quote: ... 4 µm serial sections of paraffin-embedded slides were incubated with rat anti-mouse CD34 (MEC 14.7, monoclonal, Abcam, Cambridge, United Kingdom) at a 1:200 dilution overnight at 4° ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2020Quote: ... rat anti-ADMA (5,6, kind gift of Pr C. Haass, Munich Germany, 1/100) or rabbit anti-Ubi (Abcam, ab179434, 1/100). After 3 rinses in PBS ...
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bioRxiv - Physiology 2023Quote: ... Adjacent sections were used for immunohistochemistry with anti-VDR (1:100, rat) and anti-AMH (1:50, mouse) monoclonal antibodies (Abcam, Cambridge, UK) as per manufacturers’ protocol with modifications.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice with PBST before being incubated with the primary antibody (Mouse anti-BrdU, 1:1000, Cat. No. B35128, Invitrogen; or Rat anti-BrdU, 1:1000, Cat. No. ab6326, Abcam, RRID: AB_305426) and the secondary antibody (Donkey anti-mouse 488 or 568 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: Choline and acetylcholine were quantified in rat cortical tissue from all experimental groups using a choline/acetylcholine assay kit (ab65345, Abcam, Cambrige, UK). 10 mg of cortical fresh tissue were harvested ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006, Donkey anti Rabbit 647 Abcam ab150075,). Slides were again washed 3 times 5 minutes each with PBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonyl residues in were quantified in 10 μg of the cell lysates using the Protein Carbonyl Assay Kit (Abcam, Boston, MA, USA, ab178020). Derivatization and neutralization were carried out according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... To be included antibodies had to be available in a protein free format (except for GPS2, which we purified using an Abcam Protein G purification kit). All antibodies selected were known to be specific ...
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bioRxiv - Neuroscience 2019Quote: ... with 0.3% Triton X for one hour before being staining in in PBS with 0.3% Triton X with rat anti-BrdU (Abcam: 6326 at 1:200), and mouse anti-NeuN (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the sections were incubated with histofine simple stain kit Rat MAX-PO(G) (414331F, Nichirei, Tokyo, Japan) or goat anti-chicken IgY conjugated biotin (ab6876, abcam plc, Cambridge, UK) for 30 minutrs at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then washed twice with PBS and resuspended in goat anti-rat IgG Alexa Flour 647 secondary antibody (Abcam, Cambridge, United Kingdom) diluted 1:1000 in Blocking Buffer and incubated at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... SAA and perilipin were performed using a rat anti-mouse F4/80+ antibody and rabbit anti-mouse perilipin (both 1:100 dilution, Abcam®, Cambridge, UK), and a rabbit anti-mouse SAA (1:200 dilution ...
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bioRxiv - Physiology 2020Quote: ... for 1 h followed by a 72 h incubation with primary antibodies as appropriate: rabbit anti-IBA1 at 1:1000 (Wako, 019-19741, Madison, WI) and rat anti-TMEM119 at 1:250 (abcam 209064, Cambridge, UK) [24 ...
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bioRxiv - Cell Biology 2021Quote: ... The primary antibodies rat monoclonal anti-CD13 (Novus, NB100-64843, 1:500) and rabbit monoclonal anti-Sox9 (Abcam, Cat. No. ab185966, 1:500) were diluted in Tx buffer (0.2% gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... and the last sample was added with 1 μL of anti-c-Myc rat IgG1 (1 mg/mL; clone JAC6, Abcam #ab10910, RRID: AB_297569). The antibody binding was performed for 2–3 h at 4°C on a rotator ...
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bioRxiv - Genetics 2019Quote: ... Slides were immunostained overnight at 4°C or 4h at 30°Cwith a mixture of the two primary antisera diluted in blocking buffer [rat anti-tubulin (Abcam ab6160 1:1250) and rabbit anti-MYC (Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fibers were incubated with mouse-FITC-anti-bromodeoxyuridine (detects IdU, B44, Becton Dickinson, 1:50) and rat-anti-bromodeoxyuridine (detects CldU, abcam ab6326, 1:100), at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated at room temperature for 1 hour with mouse monoclonal primary antibodies against rat substance P (1:300 dilution, ab14184, Abcam, Cambridge, MA, USA) or normal mouse serum (ab7486 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times for 10 min with 1 ml of PBS and then incubated with 500 µl of secondary antibody containing donkey anti-rat IgG Alexa Fluor 594 (ab150156, Abcam, diluted 1:300) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were incubated with primary antibody mix of mouse anti-BrdU which recognizes IdU (Becton Dickinson #347580, 1:250) and rat anti-BrdU which recognizes CldU (Abcam #6326, 1:250) diluted in blocking buffer for 2.5h at RT in a dark humid chamber ...
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bioRxiv - Cancer Biology 2021Quote: ... or TATA binding protein (Cat. No. 51841, Abcam, Cambridge, MA) were used to ensure equal loading of proteins in each lane.
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bioRxiv - Molecular Biology 2020Quote: ... 20 μg of protein were combined with Incubation Solution (Abcam) to a total of 1 mL ...
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bioRxiv - Cell Biology 2020Quote: ... anti-Nuclear Pore Complex proteins (Mab414, Abcam, ab24609 (1:200)) ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken anti-green fluorescent protein (GFP, 1:2500, Abcam, ab13970), guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3 ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken anti-green fluorescent protein (GFP, 1:5000, Abcam, AB13970), goat anti-hedgehog-interacting protein (HHIP ...
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bioRxiv - Cell Biology 2022Quote: ... microtubule-associated protein 2 (MAP2) at 1:1000 (ab92434; Abcam); Ras-related protein Rab-7a (Rab7 ...
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bioRxiv - Cancer Biology 2019Quote: ... polyclonal rabbit anti-human Nuclear Mitotic Apparatus Protein (NuMA; Abcam Cat# 97585 Lot# B115626 RRID ...
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bioRxiv - Biophysics 2020Quote: ... and polyclonal antibody against myelin binding protein (MBP) (Abcam, UK). Then ...
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bioRxiv - Pathology 2019Quote: ... and glial fibrillary acidic protein (GFAP, 1:1800; #ab68428, Abcam) that label neurons and astroglia ...
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bioRxiv - Molecular Biology 2019Quote: ... Rabbit monoclonal antibody against human P16 protein (ab108349, Abcam, Britain) was used in the Western blot assay ...
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bioRxiv - Microbiology 2021Quote: ... Protein bands were stained with an InstantBlue Coomassie solution (Abcam) and band intensity was quantified using Image Studio Lite version 5.2 (LI-COR Biosciences) ...
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bioRxiv - Neuroscience 2021Quote: ... CC1 which recognizes the QKI protein (Abcam 16794; 1:1000), Caspase-3 (Cell Signaling 9662 ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-glial fibrillary acidic protein (GFAP) (1:1000; Abcam, UK) was used as a control antibody for validation of succeeded vessel enrichment (Supplemental fig ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit anti-SARS-S protein antibody (1:3,000; ab252690; Abcam), rabbit anti-MeV-N protein polyclonal antibody (1:5,000 ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 μg of total protein and anti-mCherry (Abcam 183628) at a 1:200 dilution was used ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-Amyloid precursor protein (App) (1:200, abcam, ab32136); goat anti-Carboxypeptidase E/CPE (Cpe ...
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bioRxiv - Neuroscience 2022Quote: ... glial fibrillary acidic protein (GFAP, 1:400; Abcam Cat# ab68428), S100 calcium binding protein B (S100B ...
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bioRxiv - Neuroscience 2022Quote: ... and myelin protein zero (MPZ, 1:200; Abcam Cat# ab183868) were used ...
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bioRxiv - Genetics 2022Quote: ... for mammalian protein blots and PGK1 (Abcam ab113687, 1:3,000) for yeast protein blots ...
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bioRxiv - Developmental Biology 2022Quote: ... and to green fluorescent protein (1:200 dilution; ab13970, Abcam), and secondary antibodies included anti-chick or anti-mouse conjugated either with AlexaFluor 488 (1:500 dilution ...