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Citations for abcam :
1251 - 1300 of 1351 citations for Recombinant Human TNFRSF13B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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bioRxiv - Biochemistry 2024Quote: ... Wells were then washed with TBST (0.1% tween 20) and then incubated with an anti-human IgG antibody conjugated to HRP (Abcam, ab102420) for one hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and then incubated with 2% donkey serum followed by anti-human xanthine oxidase (1:1000; ab133268, Abcam, UK; RRID: AB_11154903), for 30min at 21 °C ...
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bioRxiv - Immunology 2023Quote: ... supernatants from the cocultures were tested in a hemoglobin release assay at a dilution of 1:1600 (Human Hemoglobin ELISA kit, Abcam) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The human origin of all patient-derived organoid xenografts (PDOXs) was confirmed by staining with an antibody recognizing human mitochondria (ab92824, Abcam).
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bioRxiv - Immunology 2022Quote: ... Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG/M antibody (Abcam) for 1h ...
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bioRxiv - Systems Biology 2023Quote: ... PVDF membranes were blocked and stained with total OXPHOS human western blot antibody cocktail (Abcam Cat# ab110411, RRID:AB_2756818, 1:500). Blots were visualized using an Odyssey infrared imaging system (RRID:SCR_022510 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then incubated in a humidified chamber overnight at 4°C with primary antibodies (1:50 dilution): rabbit anti-human CD20 (ab78237, Abcam), rat anti-mouse Fibroblast Marker ER-TR7 (sc73355 ...
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bioRxiv - Cancer Biology 2023Quote: ... Labelling of c-Myc positive cells was performed with human c-Myc antibody (Abcam rabbit ab#32072 dilution 1:200) and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633) ...
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Synthetic Biology 2023Quote: The serum concentration of His-tagged PRL N59D was determined using a quantitative ELISA as follows: 50ul of 0.1ug/mL anti-human PRL antibodies (Abcam; ab244037) were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates for 1hr at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... ATP5a and VDAC1 were all validated in IHC in paraffin embedded tissues and tested in human samples according to the supplier (Abcam).
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bioRxiv - Cancer Biology 2024Quote: ... Cytokine concentrations were quantified using the human Cytokine Antibody Array following the manufacturer’s protocol (#ab133997, Abcam Inc., Toronto, ON, CAN). 1 mL of clarified media was applied per cytokine membrane ...
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bioRxiv - Neuroscience 2024Quote: ... Plasma GLP-1 concentrations were analyzed by an experimenter blinded to treatment conditions in the DRC Radioimmunoassay and Biomarkers Core at Penn using the Human GLP-1(7-36) ELISA Kit (ab184857; Abcam) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... rehydrated tissue was stained using an antibody for human mitochondria cytochrome c oxidase subunit II (MTCO2; 1:1000, rabbit polyclonal antibody, Abcam). Wash with PBS and stain fluorescence-tagged rabbit secondary antibodies (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Protein-nucleosome binding assays were performed by incubating the abovementioned purified Pioneer-seq nucleosome library with human TP53α (Abcam ab84768), TAp63α (Origene TP710041) ...
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bioRxiv - Neuroscience 2021Quote: ... Microglia were probed with a 24h incubation at 4°C with either a rabbit antihuman IBA1 antibody diluted in blocking solution (1:200, 019-19741, Wako chemicals, USA) or a goat anti-human IBA1 antibody (1:200, ab5076, Abcam, UK). We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250 ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Cancer Biology 2019Quote: ... surface expression of iNKG2D was correlated with GFP expression using a MicAbody and detecting with PE-anti-human kappa chain (Abcam #ab79113) or by directly conjugating the Rituximab-MicAbody to Alexa Fluor 647 (Alexa Fluor Protein Labeling Kit #A20173 ...
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bioRxiv - Neuroscience 2020Quote: ... The MJFF rabbit monoclonal Rab12 pSer105 (equivalent phosphorylation site to human pSer106) antibody was described previously [21] and is available from Abcam (ab256487). Recombinant anti-LRRK2 pSer1292 MJFR-19-7-8 (ab203181) ...
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bioRxiv - Microbiology 2020Quote: ... peroxidase-labeled (Cat. 04-10-06, KPL, USA) or goat anti-human IgA alpha chain (alkaline phosphatase) (Cat. Ab97212, Abcam, UK), diluted 1:1000 in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were used: C-terminus (aa 450-499) of human Cdc20 (C-term Ab, 1:500, Abcam, ab26483), N-terminus (aa 1-175 ...
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bioRxiv - Cell Biology 2021Quote: ... cut based on the molecular weights indicated by the prestained marker and then incubated overnight on a shaker at 4 oC with rabbit anti-human STEAP3 (1:1000) (abcam, 151566), mouse anti-human p53 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... Immunostaining was performed using a rabbit polyclonal to human SC lateral element protein SCP3 (1:500 dilution; Cat# ab15093, Abcam; RRID:AB_301639) and a Cy3-labeled donkey anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: Supernatant was harvested from HeLa tBID2A cells as described above and used to probe a Human Growth Factor Antibody Array (Abcam, ab134002) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the protein expression was verified by transient transfection and western blot detection with goat anti-human polyclonal horse radish peroxidase (HRP) conjugated antibody (Abcam ab98567). A stable Drosophila S2 cell line for SALM3 LRR expression was then generated for the protein purification ...
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bioRxiv - Neuroscience 2021Quote: ... NT-3 was measured by standard ELISA according to manufacturer’s instructions (ab100615 – Neurotrophin 3 human ELISA Kit, abcam, Cambridge, United Kingdom).
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bioRxiv - Immunology 2021Quote: ... This spin was repeated and re-suspended in 200 μL of CBB containing FITC rabbit anti-human C3c pAb (Abcam, UK) a 500-fold dilution ...
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bioRxiv - Immunology 2021Quote: ... Grids were permeabilized and blocked with 0.1% Saponin/1% cold-water fish skin gelatin (Perm buffer) for 10 min and first stained with anti-human CD63 (Abcam, ab59479) antibody (1:10 ...
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bioRxiv - Cell Biology 2020Quote: ... Human and mouse lung sections were stained for colocalization with primary and secondary antibodies: ANT1 (Abcam #ab102032 rabbit polyclonal, 1:1000), pan ANT (anti-ANT1/2/3 Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse monoclonal anti-human-ATM(phospho-S1981) which stains the homologous mouse ph-ATM(phosphor-S1987) was used at 1:500 (Abcam ab36810). The slides were incubated in biotin-conjugated secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1 h and then incubated overnight at 4 °C with primary antibodies: rabbit anti-human IRS2 (ab134101; Abcam, Cambridge, UK) and mouse anti-human active β-catenin (05-665 ...
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bioRxiv - Cell Biology 2022Quote: ... Amphiregulin concentrations in HT-22 cell fractions were determined by using a Human Amphiregulin ELISA kit (ab222504; detection range: 18 – 600 pg/mL; Abcam, Netherlands). Protein concentrations were measured using RC DC Protein Assay (5000120 ...
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bioRxiv - Pathology 2023Quote: ... Sections were incubated with primary antibodies O/N at 4°C (Rabbit anti-human Lyve-1, Fitzgerald; Goat anti-murine Lyve1, R&D AF2125; rabbit anti-CD31, abcam Ab28364) and washed three times in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then processed for immunostaining by overnight incubation at 4°C in the primary antibodies of anti-Human phospho alpha-synuclein (Ser129) (1:2000, Cat# ab51253, Abcam, RRID:AB_869973), anti-Iba-1 (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies and dilutions for human muscle cross section imaging: Donkey anti-mouse (Alexa Fluor® 488) 1:500 (Abcam, #ab150105), donkey anti-goat (Alexa Fluor® 594 ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Immunology 2023Quote: ... was performed on the plasma samples from the donors obtained from the blood bank using Human Anti-Cytomegalovirus IgG ELISA kit (abcam, #ab108724) according to the manufacturer’s protocol to check for the donors’ sero-status ...
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bioRxiv - Bioengineering 2024Quote: ... cells were immuno-stained overnight at 4-8°C with rabbit anti-human IRF1 antibody (1:1000, ab243895, Abcam, Cambridge, UK) or rabbit anti-human pSTAT1 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... After SDS-PAGE and proteins transferred on PVDF membranes were incubated overnight at 4°C with Total OXPHOS Human WB Antibody Cocktail (Abcam, UK) and subsequently detected with HRP-conjugated goat anti-rabbit IgG secondary antibody (1:4000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blotted overnight at 4°C with the following primary antibodies: total OXPHOS human antibody cocktail (Abcam, 1:5000 dilution), TOM20 (Santa Cruz Biotechnology 1:5000) ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (50 μm) of skin were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam) at 1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice with PBSF and resuspended in 200 μL PBSF with 1:1000 goat anti-human antibody conjugated to AlexaFluor647 (Abcam, ab190560) and incubated for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... cryosections (10 μm) of DRG were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM)21,22 were used to study the effects of α-MSH (abcam, # ab120189) and selective MC-R agonists in vitro ...