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Citations for abcam :
1251 - 1300 of 1404 citations for Recombinant Human FAS His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Duke University] and GAPDH (mouse anti-human GAPDH, 1:10,000, 1 hr at RT; Cat: ab8245, Abcam, Waltham, MA). Primary antibodies were detected using donkey anti-rabbit IRDye® 800CW IgG (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Membrane was then incubated in 1:5000 primary antibody (HRP-conjugated Goat pAb to human IgG, Abcam, cat.: 7153) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and the bottom half was reacted against a rabbit polyclonal antibody directed to human histone H3 (Abcam, cat. no. ab1791) at 1:40,000 ...
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bioRxiv - Immunology 2021Quote: ... monoclonal rat anti-human somatostatin (dilution 1:100; MAB2358, R&D) and monoclonal rabbit anti-chromogranin (dilution 1:400, ab15160, Abcam) ON ...
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bioRxiv - Bioengineering 2019Quote: ... both rabbit anti-human (Rockland 600-401-104, 1:200, for collagen II, Abcam ab138492, 1:1500 for collagen I) were applied in 1 % NGS ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells expressing MYO19-GFP and/or mchr-Miro2 constructs were assayed for endogenous MYO19 expression using a rabbit α-human MYO19 antibody (0.9μg/ml, abcam #174286). The day after transient transfection ...
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bioRxiv - Bioengineering 2019Quote: Extracellular domain Fc fusions for human CTLA4 (CTLA4-Fc) and ErbB2 (ErbB2-Fc) were purchased from Abcam (ab180054 and ab168896). These ligands were biotinylated by the EZ-link® Sulfo-NHS-LC-Biotin according to the manufacturer’s protocol (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies anti-Aβ(1–16) (Clone 6E10, 1:100, #803001, Biolengend) and anti-(human)SYT11 (1:100, ab204589, Abcam) were used ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were blocked with 1% BSA-0.3 M glycine in PBST and incubated with mouse anti-human integrin αvβ3 IgG (1:200, monoclonal, LM609, Abcam, ab190147) in 1% BSA-PBST overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and goat anti-human IgG (ab97225) conjugated with horseradish peroxidase (HRP) purchased from Abcam. Nickel coated clear 96-well plates were purchased from Thermo Scientific (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Recombinant antibody generated from the full-length SARS-CoV-2 nucleocapsid protein (product ab272852) and anti-human secondary antibody were purchased from Abcam. Proteins were resolved on a 4-12% Bis-tris gel using SDS-PAGE and transferred to a PVDF membrane (pore size 0.45 µm) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).
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bioRxiv - Biochemistry 2020Quote: ... HRP-labeled goat anti-human IgG was used at 1:1,000 dilution and the color development was with TMB ELISA Substrate (Abcam, ab171523) The experiment was performed in duplicate and the background subtraction was done by subtracting the corresponding binding signals obtained with BSA-immobilized wells ...
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bioRxiv - Cell Biology 2021Quote: ... bound IRE1α LD-Fc was transferred after washes to a PVDF membrane and detected with anti-human Fc antibody (ab977225, Abcam), ECL solution (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated for 24 hours with the following antibodies (1:1000 v/v dilution in TBST): i) mouse anti-human OxPhos cocktail (Abcam; Cambridge ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-mouse Mastl (AP14289c) was from Abgent and anti-human MASTL was generated at the CNIO (available from Abcam, ab166647). Anti-pan-B55 (sc-81606 ...
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bioRxiv - Immunology 2022Quote: ... was used to detect the presence of human CXCL10/IP-10 in the supernatants of infected or stimulated A549 cells using TMB (Abcam) as the substrate solution and 0.3 M H2SO4 as the stop solution.
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bioRxiv - Immunology 2022Quote: ... For the analysis of cell surface molecules cells were stained with APC anti-human CD48 (MEM-102; Abcam, Cambridge, UK); APC-anti human 2B4/CD244 (2-69 ...
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bioRxiv - Bioengineering 2022Quote: ... Antibodies were as follows: i) for human cells: mouse IgG1 anti-CD44/CD73/CD90/CD45/CD105/CD14/HLA-DR (all from Abcam); ii ...
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bioRxiv - Cell Biology 2022Quote: ... The following commercial polyclonal and monoclonal antisera were used (m, mouse; h, human and r, rat proteins): anti-LIMPII (ab165222) was from Abcam; anti-Calnexin (2679) ...
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bioRxiv - Microbiology 2021Quote: ... Beads were next washed twice with 200 μl wash buffer and resuspended in 100 μl FITC-conjugated rabbit anti-human C3c polyclonal antibody (Abcam) diluted 1:500 in BB and incubated in the dark at 25°C for 20 min ...
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bioRxiv - Physiology 2021Quote: ... Schiaffino and obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the University of Iowa Department of Biology as well as laminin (#11575; Abcam) and 10% goat serum ...
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bioRxiv - Cancer Biology 2020Quote: ... The stainings were performed using either CDK6 monoclonal rabbit anti-human antibody (Clone EPR4515, dilution 1:250, Abcam, Cambridge, UK) or Cyclin D2 monoclonal rabbit anti-human antibody (Clone D52F9 ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion by HepG2 cells encapsulated in liver models with or without vasculature channels was quantified using ELISA (Human Albumin ELISA kit, Ab 108788, Abcam). Liver models (polymer composition ...
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bioRxiv - Cancer Biology 2021Quote: ... based on hematoxylin and eosin (H&E) staining and nuclear immune-positivity for human-specific NCL (ab13541, Abcam, Cambridge, UK) as previously described [21] ...
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bioRxiv - Cell Biology 2019Quote: ... RUNX-2 positive cells were similarly stained using a mouse anti-human RUNX-2 monoclonal antibody (ab76956, Abcam, Cambridge, UK), which was revealed using an Alexa Fluor®488 goat anti-mouse IgG2a secondary antibody (A-21131 ...
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bioRxiv - Bioengineering 2019Quote: Levels of brain natriuretic peptide (BNP) secreted in the media were quantified by Human BNP ELISA Kit (Abcam, cat# ab193694) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cells were blocked with 5% BSA in PBST for 30 min at RT and incubated with primary antibody (monoclonal mouse anti human SC35, Abcam) at 1:250 dilution for 1 hr at 37°C ...
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bioRxiv - Immunology 2020Quote: ... day 1st-stimulated CAR T cells were co-cultured with γ-irradiated target cells at a 1:3 E:T ratio in the presence of 10 μg/ml plate-bound human CD226 blocking monoclonal antibody (clone DX11, Abcam, UK). Ultra-LEAF™ purified mouse IgG1(Clone MOPC-21 ...
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bioRxiv - Molecular Biology 2021Quote: Human RIPK3 rat monoclonal 1H2 (generated in-house)(Petrie et al, 2019b) Human phospho-MLKL (Ser358) rabbit monoclonal ab 187091 (Abcam) VDAC1 rabbit polyclonal AB10527 (Millipore)
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bioRxiv - Immunology 2020Quote: ... The following fluorochrome conjugated rabbit anti-human antibodies were also used in the intracellular staining: FITC anti-GLUT3 (polyclonal) and PE anti-GLUT1 (EPR3915) from Abcam. Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Immunology 2020Quote: Cytokine levels in the cellular supernatant were evaluated using the FirePlex-HT assay system with the Human Cytokines FirePlex-HT Panel 1 (ab234897; Abcam). FirePlex-HT immunoassays quantify up to 10 protein analytes per sample from low sample inputs in 384-well plate format ...
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bioRxiv - Microbiology 2020Quote: ... then incubated with 0.1μg/ml HRP-conjugated Goat Anti-Human IgG H&L or HRP-conjugated Goat Anti-Rabbit IgG H&L (abcam) in 0.5% BSA in PBST for 1h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with TBST and incubated with HRP-conjugated Goat-anti-human Fc antibody (Abcam, ab99759, 1:10000) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... ABCA1 (ATP-binding cassette lipid transporter marker of multivesicular bodies; mouse anti-human ABCA1; 5 μg/ml; Abcam Cat. #ab18180) and ...
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bioRxiv - Cancer Biology 2020Quote: ... rehydrated through an alcohol gradient to water and antigen retrieval in citrate buffer pH 6.0 was used for the human nucleolin antibody at 5.0 g/mL (ab13541) (Abcam, Canbridge, MA). Endogenous peroxide activity and nonspecific binding was blocked with 3%(v/v ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Bioengineering 2022Quote: ... for detection (brown signals) of collagen type I (1:100 anti-human collagen I alpha-1 1200-1450 fragment (ab233080, Abcam) compatible to pig elastin [45] ...
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bioRxiv - Genomics 2022Quote: We determined the cytomegalovirus (CMV) serostatus of AFB (n = 80) and EUB (n = 80) donors with a human anti-IgG CMV ELISA kit (Abcam) on plasma samples ...
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bioRxiv - Microbiology 2022Quote: ... tween 20 in PBS (PBST) three times followed by incubation with goat F(ab’)2 anti-human IgG (Fab’)2-horseradish peroxidase (Abcam) at a 1:10,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: Human serum levels of LBP were assayed using the Human LBP ELISA kit standard sandwich enzyme-linked immunosorbent technology and following the manufacturer’s protocol (Abcam, ab213805). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... and anti-human MT1-MMP rabbit monoclonal antibody EP1264Y against the catalytic domain (Abcam, catalog no. ab51074, 1:1000 dilution) were used followed by anti-rabbit secondary antibodies (Li-COR ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibodies (Ab) used in this study: rabbit polyclonal Ab to human serum albumin (Abcam, catalogue no. ab2406, 1:400), goat polyclonal antibody to E-cadherin (R&D systems ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were probed with the following primary antibodies: rabbit polyclonal anti-calreticulin (1:1000) (ab19261, abcam; to detect human CRT); chicken polyclonal anti-calreticulin (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were fixed at day 7 of culture and stained with CD31 human antibody (Abcam; ab134168; AF488-conjugated; 1:100) and imaged with an Inverted confocal microscope ...
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bioRxiv - Biochemistry 2024Quote: ... Wells were then washed with TBST (0.1% tween 20) and then incubated with an anti-human IgG antibody conjugated to HRP (Abcam, ab102420) for one hour at 37°C ...