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Citations for abcam :
1251 - 1300 of 10000+ citations for Mouse IgG2b Isotype Control Antibody A 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... The Rabbit IgG control antibodies for ChIP-qPCR experiments were ab172730 (abcam, GR3298823-2, 2ug per 80ug chromatin), and Normal IgG Rabbit 2729 (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... Loading was additionally controlled by detecting GAPDH as a control (Anti GAPDH antibody sourced from abcam; catalog ab181602).
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bioRxiv - Neuroscience 2021Quote: ... 0,3% Triton in PBS and incubated overnight with primary antibody (Guinea pig anti-Iba1, 1:500 [abcam] and mouse anti-NeuN, 1:500 [abcam]). Slides were washed 3 × 30 min and incubated with secondary antibodies (Donkey anti-mouse 405 ...
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bioRxiv - Microbiology 2021Quote: ... were immunoblotted for ACE2 and β-actin using primary antibodies (1:500 polyclonal goat anti-human ACE2, R&D Systems, AF933; 1:1000 monoclonal mouse anti-β-Actin, Abcam, AB8226) followed by secondary antibodies (1:4000 donkey anti-goat ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:1000 dilution) at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... washed four times with PBS-T and 20 μL of polyclonal HRP-conjugated detection antibodies (goat anti-mouse, #G-21040, Thermo Fisher at 1:1,000 dilution or goat anti-human, #ab7153, Abcam at 1:10,000 dilution) were added to each well and incubated for 30 min (anti-mouse ...
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bioRxiv - Genomics 2020Quote: ... and IgG control (ab37415, Abcam) as previously described [55] ...
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bioRxiv - Genomics 2022Quote: ... Rabbit control IgG (ab46540, Abcam), Mouse control IgG2a (ab18413 ...
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bioRxiv - Biochemistry 2022Quote: ... β-Tubulin loading control (Abcam, ab6046), and goat anti-rabbit (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... with GAPDH as control (Abcam) followed detection of bands with enhanced chemoluminescence (GE Healthcare Bio-Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... or control IgG (Abcam, ab37415). Magnetic Protein G Dynabeads™ are used for pull-down according to manufacturer’s instructions (Thermo Scientific™) ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... and IgG control(Abcam; 172730). Sample processing was performed in a 96 well plate using 100K Con-A bound nuclei per reaction on a Beckman Coulter Biomek liquid handling robot according to the AutoCUT&Tag protocol available from the protocols.io website (https://doi.org/10.17504/protocols.io.bgztjx6n ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse Iba1 (019-19741, Wako Chemicals, 1:500) and anti-mouse CD45 (ab23910, Abcam, 1:500); panel 3 = anti-mouse BrdU (ab8152 ...
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bioRxiv - Developmental Biology 2019Quote: ... The remain precleared lysates were incubated with specific antibody (anti-HA antibody, anti-DAZL antibody) or its corresponding IgG (mouse IgG, ab37355; rabbit IgG, ab37415; Abcam) 4 hrs at 4°C on a rotor ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was revealed with PE-conjugated goat anti-mouse antibodies (2.5 μg/mL, Abcam #ab97041). The negative control consisted of bacteria in 0% plasma (100% PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Neuropeptide antibodies raised in mouse were combined with rabbit recombinant anti-alpha tubulin antibody (abcam, ab190471). All primary antibodies were diluted 1:100 in blocking solution ...
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bioRxiv - Cell Biology 2019Quote: ... α-Biotin mouse monoclonal antibody and α-RPB1 (p-Ser2) rabbit polyclonal antibodies are from Abcam. α-V5 rabbit polyclonal antibody is from Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with primary antibody (Anti-Myc tag antibody, mouse monoclonal [9E10], Ab32, Abcam), diluted 1:1000 in 5% milk in TBST at 4°C overnight while rocking ...
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bioRxiv - Cell Biology 2020Quote: ... Western blot signals were detected using 1:5000 dilutions of either goat anti-mouse or goat anti-rabbit secondary antibodies conjugated to alkaline phosphatase (Abcam) and CDP-Star chemiluminescence kit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed and permeabilized cells were then incubated with 1:400 dilution of Alexa Fluor 647 conjugated mouse anti-human cytokeratin 5 antibody (Abcam) for 24 hours at 4C in permeabilization buffer ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with 1 μg/mL of mouse anti-β III-tubulin antibody (RRID: AB_2256751, cat. no. 78078, Abcam) in blocking solution overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... BSA TBST buffer and the following primary antibodies diluted in blocking buffer were used: Mouse anti-GAPDH (1/5000, Abcam), mouse anti-B-catenin (sc7963 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were applied over night at a dilution of 1:100 for SARS-CoV S protein (monoclonal mouse, ab272420, Abcam; microwave ...
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bioRxiv - Microbiology 2020Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: ... Fluorescently labeled secondary antibodies were diluted 1:500 in PBS-T as follows: single labeling: Alexa 594 (anti-mouse, ab150108 Abcam); double labeling ...
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bioRxiv - Plant Biology 2021Quote: ... the PVDF membrane was then incubated with a goat anti-mouse immunoglobulin G AP-conjugated secondary antibody (ab97020, 1:5000, Abcam). AP activity was detected by BCIP/NBT kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated for 24 hours with the following antibodies (1:1000 v/v dilution in TBST): i) mouse anti-human OxPhos cocktail (Abcam; Cambridge ...
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bioRxiv - Microbiology 2022Quote: ... The secondary antibodies used included: goat anti-rabbit IgG HRP-conjugated and goat anti-mouse IgG HRP-conjugated secondary antibody (1:5000, Abcam), Alexa Fluor 555 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-mouse Alexa Fluor® 594 for the 8-oxo-dG antibody (abcam, Cat# ab150120, RRID AB_2650601, 1:500), and 2% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibody cocktail consisted of mouse anti-glutamic acid decarboxylase 65 (GAD65, abcam, Cat# ab26113, RRID: AB_448989, 1:500), mouse anti-glutamic acid decarboxylase 67 (GAD67 ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were used at 1:500 (Goat Anti-Mouse IgG H&L Alexa Fluor® 647 (ab150115 Abcam)) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450, 1:100 or mouse monoclonal anti-HA, Abcam ab18181 ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... according to the manufacturer instructions and stained with primary mouse monoclonal antibodies for SQSTM1/p62 (Abcam, cat. ab56416, 1:1000), LAMP1/CD107a ...
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bioRxiv - Genomics 2019Quote: ... Coverslips were washed three times for 5 minutes each with PBT and incubated with diluted secondary antibody (anti-mouse labelled with Alexa Fluor 488, 1:800, ab150113, Abcam; anti-rabbit labelled with Alexa Fluor 647 ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 h at room temperature and then incubated overnight at 4°C with primary antibody: mouse anti-GAPDH (1:5000, Abcam) or rabbit anti-parvalbumin (1:10000 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies where used for western-blotting at 1/1000 dilution: anti-Rpb1-CTD clone 8wG16 (mouse, monoclonal, Abcam), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then blocked for 1 hour with a 5% milk-TBS solution and incubated overnight at room temperature with a 1:1,00 dilution of mouse monoclonal antibody against TPP1 (Abcam ab54685) in 5% milk-TBS ...
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bioRxiv - Microbiology 2019Quote: ... cells were incubated for 30 min with Alexa488-conjugated goat anti mouse and Alexa546 conjugated donkey anti rabbit secondary antibodies (1/400e, AbCam). After washing ...
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bioRxiv - Genetics 2019Quote: ... The cells were fixed in 4% paraformaldehyde and permeabilized with saponin and lysosomes were labeled using 10 μg/mL dilution of FITC-labeled antibody against mouse Lamp-1 (ab24871, abcam), a lysosomal structural protein ...
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bioRxiv - Biochemistry 2021Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), CENP-E (mouse ...
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bioRxiv - Cell Biology 2021Quote: The following primary antibodies were adopted for IHC and IF on mouse and human tissues: rabbit polyclonal ACE2 (1:500 pH8, ab15348, Abcam), rabbit polyclonal Prosurfactant Protein C (1:200 pH9 ...
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bioRxiv - Pathology 2021Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-mouse MMP-13 (1/6000; ab39012; Abcam) and MMP-1 (1/500 ...
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bioRxiv - Cell Biology 2020Quote: ... blocked with Odyssey Blocking Buffer (Li-Cor 927-40000) and incubated with CENP-A Mouse antibody (1:500; Ab13939; Abcam) in 5% BSA TBS with 0.1% Tween 20 and detected with IRDye 800CW and 680LT secondary antibodies using the Odyssey Infrared Imaging System (Li-Cor).
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bioRxiv - Cell Biology 2020Quote: ... TZM-bl cells were similarly transfected followed by staining with 1:50 dilution of anti-mouse monoclonal CD4-APC conjugated antibody (Abcam) or isotype control (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... then were washed and was incubated with 1:200 secondary antibody donkey anti–mouse-Alexa Fluor 568 (ab175700, Abcam, USA). The non–permeabilized samples stained for accessible E-cadherin were permeabilized with PBS-0.2% Triton 100-X for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Tween-20 and 5% (w/v) milk powder containing 1:5,000 horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibody (AbCam ab205719) and incubated on a shaking platform for 1 hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then washed 3x with PBST and incubated 1 hr at 22°C in 100 µL anti-mouse IgG AlexaFluor 594 secondary antibody (ab150116, Abcam) diluted 1:200 in PBST + 1% bovine serum albumin + 10% goat serum ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245 ...
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bioRxiv - Cancer Biology 2023Quote: ... we also included a subsequent incubation with the secondary rabbit anti-mouse IgG antibody (used at a 1:100 dilution; Abcam, cat ...