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Citations for abcam :
1201 - 1250 of 1350 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 hours at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (dilution 1:5000, Abcam #ab6789). After washing again ...
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bioRxiv - Immunology 2024Quote: ... sections were repaired using EDTA antigen recovery buffer and incubated overnight at 4 °C with anti-dengue virus 1+2+3+4 primary antibody (Abcam, ab26837). Next ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: To quantitatively measure intracellular reactive oxygen species in live cell samples we have used cell permeant reagent 2’,7’–dichlorofluorescin diacetate (H2DCFDA) from Cellular ROS Assay Kit (Abcam, ab113851) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 1% normal goat serum (NGS) for 1 hour and incubated overnight with primary recombinant anti-prolactin receptor antibody [EPR7184(2)] (abcam, ab170935) at 1:50 in 1% BSA ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubations were performed overnight at 4°C in PBS-T with 2% NGS plus one or more of the following antibodies: polyclonal rabbit anti-GFP (1:800, Abcam #ab290), monoclonal mouse anti-Abd-B (1:500 ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Western blot analysis was done as described (IHARA AND NISHIWAKI 2007) using anti-NID-1 (2 μg/ml) (KUBOTA et al. 2008) and anti-α-tubulin (DM1A, 1:1000; abcam), anti-GFP (3E6 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated in 50 μL of 2% goat serum in PBS containing mouse monoclonal anti-NR2A antibody (abcam #240884, 1:200) and rabbit monoclonal anti- Na+/K+-ATPase ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with the SARS-CoV-2 Spike Antibody Mouse Mab (40592-MM117, SinoBiological, China) and HRP-conjugated goat anti-mouse IgG (ab6789, abcam, UK). Detection was carried out using an Ultra High Sensitivity ECL Kit (HY-K1005 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocked cells were then incubated for 1 hour at room temperature with primary antibodies anti-Isl1/2 (Abcam ab109517, 1:2000), anti-Chx10 (Santa Cruz Biotechnology sc-365519 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was conducted using 2% w/v BSA in 1×PBS for 1 h and incubated with anti-Ki67 antibody (Abcam ab16667) in a 1:200 dilution ...
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bioRxiv - Microbiology 2024Quote: ... the cell lysate was split into equal volumes and combined with 2 µg mouse mAb anti-V5 (SV5-PK1) (Abcam, ab27671), mouse mAb anti-HA (clone HA-7)(Merck ...
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bioRxiv - Developmental Biology 2024Quote: ... in PBS for 2 hours at room temperature and incubated overnight at 4°C using the following concentrations: anti-H3K9me3 (1:300, Abcam ab8898), anti-H3K27me3 (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were blocked for 2 hr with 3% BSA and incubated for 1 hr with 1:500 dilution of rabbit anti-HA antibody (Abcam 20084). Cells were washed again and incubated for 1 hr with a 1:1000 dilution of anti-rabbit Alexa Fluor 488 antibody (Invitrogen A11008) ...
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bioRxiv - Neuroscience 2024Quote: ... the slices were rinsed in PBS and incubated for 2 h in goat anti-guinea pig (Alexa Fluor® 555) (1:200, Abcam, Cambridge ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membrane was then incubated in blocking buffer (5% skim milk in TBS/0.05% tween-20) followed by blotting with primary antibodies (anti-SARS-CoV-2 spike (Abcam ab275759, USA) 1:2,000 or anti-β-actin (Sigma A2228 ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked with 5% normal goat serum/2% BSA/0.1% Triton-X for 1 hour and incubated with mouse monoclonal PAX7 antibody (abcam, 1:200) and rabbit monoclonal SOX10 antibody (Cell Signaling Technologies ...
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bioRxiv - Pathology 2024Quote: 1.4 mM stock solutions of hIAPP1-37 with amidated C-terminus and a Cys-2 to Cys-7 intramolecular disulfide bridge (Abcam, UK) were prepared by dissolving 1 mg of lyophilised peptide in 99.9% DMSO/0.1% TFA ...
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bioRxiv - Neuroscience 2024Quote: Primary hippocampal neurons treated with Tris and PFFs at various time points were incubated in growth media with 1 μM Fura-2 AM (Abcam, UK) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... fixed samples were incubated with 400 µL of a 1 µg mL-1 Alexa Fluor 647 Rabbit Monoclonal anti-FGF-2 IgG (abcam, USA) solution in PBS with 10 % Goat Serum (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... cells were incubated with rabbit Human Protein Atlas antibodies (HPA072117 for NANOG, HPA058267 for POU5F1) at 2 µg/mL and mouse anti-tubulin antibodies (Abcam ab7291) at 1 µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... and then incubated overnight at 4°C with primary antibodies: Anti-beta Amyloid 1-40 [EPR23712-2] (AbCam, Cat. No. ab289991) for Aβ40 and Anti-Amyloid Beta [21F12] (Absolute Antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice with 1xTBST buffer for 5mins each and then incubated for 2 hours with alkaline phosphatase-conjugated anti-rabbit IgG (Abcam; #ab97048) at dilutions of 1:10000 in 5% milk TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... The IP was performed using Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (Abcam, ab108123; 3μg/2 X 10^6 cells) overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a cell permeable analog of 1,2-bis-(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid acetoxymethyl ester (BAPTA-AM, Abcam, 10 μM) (Tsien ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS-RFP-NLS cells were a gift from Martin Hetzer (Salk Institute, CA, USA) (17, 20) and were cultured in full DMEM supplemented with 0.5 mg/ml G418 (Abcam, ref. ab144261, lot. GR 162868-8). Cells were regularly tested for mycroplasma using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed three times in PBS containing 0.01% Triton X-100 and incubated for 2 hours with AlexaFluor conjugated donkey anti-rabbit secondary antibody (Abcam ab150073 1:1000). After secondary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the same ACE2 IHC protocol was applied to other 2 primary antibodies anti-Human ACE2: monoclonal rabbit anti-Human ACE2 (cat. Ab108252, Abcam, Cambridge, UK) diluted 1:100 in PBS 1× supplemented with 5% goat serum ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were rotated for 2 hours at 4°C in 200 μL DigWash with 1:400 Lamin B1 antibody (Abcam, ab16048, rabbit), followed by a wash step with DigWash ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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bioRxiv - Genomics 2020Quote: ... Blocking with 1% BSA/PBS for 1h was followed by incubation with the following primary antibodies for 1-2 h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... with fluorochrome for 1 hour in the dark and the samples were then mounted with Fluoroshield mounting medium with DAPI (4’,6-diamidino-2-phenylindole, Abcam, Cambridge, MA). Slides were imaged using the Keyence BZX-700 Fluorescent Microscope (UCSD Microscopy Core).