Labshake search
Citations for abcam :
1201 - 1250 of 1258 citations for Siglec 8 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... cryosections (10 μm) of DRG were stained with primary antibodies: mouse anti human IAPP antibody (1:500, Abcam Ab115766 mouse monoclonal), rabbit anti-I11 oligomer antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... after which the media in all four reservoirs was collected and analyzed with the Human Cytokine Antibody Array (Abcam, Cambridge, UK). The full list of cytokines tested in the kit is found in supporting Table S2.
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2022Quote: Protein array analysis was performed using the Human MAPK Phosphorylation Antibody Array (ab211061) according to the manufacturer’s instructions (Abcam, Boston, MA). Visualization was performed with the ChemiDoc Touch Imaging System (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM)21,22 were used to study the effects of α-MSH (abcam, # ab120189) and selective MC-R agonists in vitro ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit polyclonal anti-clathrin heavy chain (against residues 1650 to C-terminus of human clathrin heavy chain, IF 1:150) was from Abcam (#ab21679). Mouse monoclonal anti-clathrin light chain (clone X22 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies and dilutions for human muscle cross section imaging: Donkey anti-mouse (Alexa Fluor® 488) 1:500 (Abcam, #ab150105), donkey anti-goat (Alexa Fluor® 594 ...
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bioRxiv - Cancer Biology 2024Quote: The evaluation of human MMPs (Matrix Metalloproteinases) and TIMPs (Tissue Inhibitors of Metalloproteinases) was conducted following the instructions provided by the manufacturer (abcam 134004). In summary ...
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bioRxiv - Neuroscience 2024Quote: The activity of CatB was examined in the cortex of 3-month-old mice or human fibroblasts using a Cathepsin B Activity Assay Kit (Abcam, #ab65300). The tissue or fibroblasts were homogenised in CB lysis buffer and then incubated on ice for 30 minutes before being centrifuged at 15,000 x g for 5 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat. ab108252, Abcam, Cambridge, UK) diluted 1:100 in BSA 1% in PBS without Ca2+ and Mg2+ ...
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bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound antibody was removed by five 5-min washes and the membrane was incubated with alkaline phosphatase-conjugated goat anti-mouse secondary antibody or goat anti-human IgG secondary antibody (Abcam, Cambridge, UK). Membranes were washed again and then developed using SuperSignal ELISA Pico Chemiluminescent Substrate Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2019Quote: The detection of specific anti-RSV IgG antibodies in biological fluids was performed using Human Anti-Respiratory syncytial virus IgG ELISA Kit (ab108765, abcam®, Sweden), according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed once with 200 μL PBS containing 1% BSA and incubated with PE conjugated Goat F(ab’)2 Anti-Human IgG-(Fab’)2 secondary antibody (abcam, Cambridge, MA) at a final concentration of 2.5 μg/ml in 100 μL PBS containing 1% BSA per well ...
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bioRxiv - Microbiology 2020Quote: The IL-12p40 in cell supernatants of infected and uninfected macrophages were quantified using BD OpteEIA™ Human IL-12(p40) ELISA kit (Abcam, UK) as per the manufacturer’s directives.
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bioRxiv - Immunology 2020Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody (Abcam, ab98532, 1:2000) for 30 min at RT ...
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bioRxiv - Bioengineering 2020Quote: Albumin secretion was quantified in Liver-Chip effluent collected from the top channel using the Human Albumin SimpleStep ELISA® Kit (Abcam, #ab179887) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (Goat anti-GFP biotin conjugated, Vector Labs; sheep polyclonal anti-human-sema3f antibody, R&D systems; mouse anti-GADPH, Abcam) were diluted in the blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Expression of each species’ ACE2 receptor in the cells was confirmed by Western Blot analysis using antibody to human ACE2 (Abcam, Waltham, MA). Parental CRFK cells served as a control (Mock).
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bioRxiv - Microbiology 2021Quote: ... Chuncheon-si, ROK), anti-HCoV-OC43(LS-C79764, LS-bio, Seattle, WA, USA) and anti-human GAPDH antibody (ab9485, Abcam, Cambridge, UK) overnight at 4°C and then incubated with anti-mouse or rabbit IgG-HRP conjugate (209-035-082 or 309-035-003 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... After 1 hour the wells were washed and Ab binding was detected with Donkey F(ab’)2 Anti-Human IgG conjugated to phycoerythrin (Abcam cat.# ab102439). Cells were analyzed using an iQue High-Throughput Flow Cytometer (Sartorius ...
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bioRxiv - Immunology 2022Quote: Binding of TIM-4 to infected cells was tested using a TIM-4 Fc chimera composed of the extracellular domain of TIM-4 fused to the N-terminus of the Fc region of human IgG (Abcam, Waltham, MA). One μg of TIM-4 in PBS containing 1% BSA was incubated on ice for 30 minutes with 105 washed CD4 T cells infected as indicated above with SIVGAGGFP ...
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bioRxiv - Cell Biology 2019Quote: The same protocol was used for the staining of VEGF-A expressing cells using a rabbit anti-human VEGF-A monoclonal antibody (ab52917, Abcam, Cambridge, UK), which was revealed using the same secondary antibody as above ...
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bioRxiv - Biochemistry 2019Quote: ... 20 μg of total cell lysates were separated on SDS-PAGE and protein transferred to nitrocellulose membranes that were probed with antibodies against human pancreatic α-amylase (Abcam #ab21156) and ENT2 (Alomone Labs #ANT-052 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Immunology 2020Quote: TNF-α concentrations in undiluted or appropriately diluted supernatants were determined by ELISA using the SimpleStep human TNF alpha ELISA kit (abcam, Cambridge, MA), performed following the manufacturer’s instructions and with endpoint absorbance measurements at 450 nm on an Infinite M200 Pro plate reader (Tecan ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with heat-inactivated serum and visualized with a goat anti-human secondary antibody (IgG) conjugated with FITC (Abcam, Cat#ab97224).
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bioRxiv - Genetics 2020Quote: ... and incubated with rabbit polyclonal anti-PEA-15 (C-terminal amino acids 93-123 of Human PEA15) antibody ab135694 (Abcam, Cambridge, UK) at a concentration of 1:100 and anti-GAPDH antibody (MAB374 ...
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
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bioRxiv - Immunology 2024Quote: ... The antibodies were removed and the plates were washed three times with PBS + 0.1% Tween20 before adding anti-human IgG HRP conjugate (Abcam, #ab97225, 1:20000) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... blood samples from each repopulated mouse were collected via the saphenous vein and analyzed using the Human Albumin ELISA Quantitation Set (Abcam, Cambridge, UK) according to the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with primary antibodies diluted in blocking buffer overnight at 4°C - Mouse Anti-Human TRA1-81 (1:75, Abcam, AB16289#20), Rabbit Anti-Human OCT4 (1:250 ...
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bioRxiv - Genomics 2023Quote: ... cells were fixed as in smFISH and blocked with co-detection antibody diluent for 30 min at room temperature prior to incubating with anti-human SC35 (Abcam, Cat # ab11826) at 1:50 dilution overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The following primary antibodies were used for the western blot experiments: mouse monoclonal antibody against human apolipoprotein E (dilution 1:1000; Abcam, Cambridge, UK), and polyclonal rabbit antibodies against the LL37 epitope (diluted 1:1000 ...
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bioRxiv - Biochemistry 2023Quote: Cell lysates were subjected to SDS/PAGE (0.1% w/v) immunoblotting analysis using an anti-human XOR rabbit antibody (1:2000, Abcam 133268 RRID: AB_11154903). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were incubated at 4°C overnight with a monoclonal antibody to human cytokeratin antigen (Abcam, Cat #ab53280, at 1:250), or monoclonal antibody to human POSTN (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... washed three times with PBS and then 200 µl PBS containing anti-human antibodies conjugated to alkaline phosphatase (Abcam, 1:2000 dilution) was added for 1 h ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and 50 μL Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HSC70/HSP70 (clone N27F3-4, ENZO dilution 1:1.000, sample reduced with β-mercapthoethanol) and rabbit anti-human lactoferrin (polyclonal, Abcam, dilution 1:5.000), rabbit anti-human TLR3 (D10F10 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... For western blot analysis, a human polyclonal IgG anti-SARS-CoV-2 (CIGB, Cuba) and a mouse monoclonal antibody against CK2α (Abcam, Cambridge, United Kingdom) were used as primary antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... The GP-13C6 complex was identified by adding a polyclonal human anti-IgG antibody conjugated to FITC (Abcam, Cambridge, USA; Cat. No. ab97224). Cell cultures were observed under fluorescence illumination with an optical microscope (Axio Imager M2 microscope (Zeiss ...
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bioRxiv - Physiology 2022Quote: ... and then stained for αSMA overnight using rabbit anti-human αSMA antibody at a 1:500 dilution at 4°C (Abcam, San Francisco, CA). The chamber slides were incubated with a goat anti-rabbit secondary antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... according to manufacturer’s instructions using human anti-PD-L1 antibody (Avelumab, EMD Serona, 1 μg/ml) or rabbit monoclonal Na/K ATPase antibody (Abcam # ab76020, 1:100,000) as primary antibodies and peroxidase-conjugated donkey anti-human IgG-HRP (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) for 30 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
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bioRxiv - Immunology 2020Quote: The HSV-seropositivity status of donors completing the survey was confirmed by ELISA using the anti-herpes simplex virus type 1 and 2 IgG human ELISA kit (abcam, Cambridge, United Kingdom) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... A 100 μg aliquot of anti-SARS-CoV-2 N human mAb was conjugated with Alexa Fluor® 647 Lightning-Link® Conjugation Kit (Abcam # ab269823), while 100 μg of anti-SARS-CoV-2 S were conjugated with Alexa Fluor® 488 Lightning-Link® Conjugation Kit (Abcam # ab236553) ...
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bioRxiv - Genetics 2019Quote: ... washed with 0.1% PBS-Tween for three 15min incubations and then incubated with rabbit-anti-human CR1 (also known as CD35; Abcam #ab126737, 1:100) for 48hrs in 0.1% PBS-Tween on an orbital shaker at +4°C ...
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bioRxiv - Bioengineering 2022Quote: ... Each well was blocked with 5% BSA-tween solution and incubated with a primary mouse anti-human α-SMA antibody (ab7817, Abcam, Cambridge, UK) solution at a 1:100 dilution overnight at 4°C ...