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Citations for abcam :
1151 - 1200 of 2750 citations for Rat Immunoglobulin G2a IgG2a ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: The serum concentration of His-tagged PRL N59D was determined using a quantitative ELISA as follows: 50ul of 0.1ug/mL anti-human PRL antibodies (Abcam; ab244037) were diluted in PBS and used to coat MaxiSorp 96-well ELISA plates for 1hr at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Serum iron and liver and spleen non-heme iron was measured as previously described.27 Serum sTfR and hepcidin were measured by ELISA (Abcam and Intrinsic Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: Cell media testosterone levels were determined using a commercially available testosterone ELISA assay according to the manufacturer’s protocol (#ab108666, Abcam, Cambridge, UK).
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed five times with PBS-T and 100 μL of TMB ELISA Substrate Solution (Abcam, Cambridge, MA, USA) was added to all wells of the plate and incubated for 5 minutes before the reaction was stopped ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin levels were determined in duplicate using 150 μg of protein lysates per well using a sandwich ELISA assay (abcam, ab252364). Western blot and qPCR analyses were carried out as described above ...
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bioRxiv - Neuroscience 2021Quote: ... or plasma (diluted 1/125 or 1/250 in PBS) samples were employed in a C1q sandwich ELISA with a polyclonal rabbit antibody (Abcam AB71940) used for capture and a monoclonal C1q antibody (Annexon M1 ...
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bioRxiv - Cancer Biology 2020Quote: ... ELISA test for AREG levels was performed using 100ul of each sample in duplicates and following the manufacturer protocol (Abcam, Ab99975).
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bioRxiv - Microbiology 2019Quote: ... A 96-well ELISA plate (Nunclon® Immobilon) was coated with 70 µL of polyclonal rabbit anti-Salmonella antibody ab35156 (Abcam) suspended in PBS (5 µg/mL ...
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bioRxiv - Immunology 2020Quote: Concentrations of anti-CMV IgG relative to a standard curve and internal negative and positive control were determined by ELISA (Abcam, UK) and read in a microplate spectrophotometer (Bio-Rad xMark ...
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bioRxiv - Immunology 2020Quote: ... The pellets obtained after each sequential centrifugation step were resuspended in PBS and used for vesicle immunoblotting with the primary antibodies used in ELISA applications (see below) plus p-H2AX S139 (Abcam, ab26350) and BRCA1 (GeneTex ...
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bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
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bioRxiv - Microbiology 2022Quote: ... Validation of host targets for specific cytokines (IL-1β, IL-6, KC, and TNF-α) were performed by sandwich ELISA (AbCam) as indicated in our prior study (46).
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bioRxiv - Bioengineering 2023Quote: ... 1 mg/ml collagen gels were incubated with 10mM MGO and the clinically relevant MGO-derived advanced glycation end-products (AGE) hydroimidazolone MG-H1 was measured using a competitive ELISA assay (Abcam, UK) according to the manufacturer’s instructions and previously published approaches23.
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bioRxiv - Molecular Biology 2021Quote: ... Specimens were fixed in 4% formaldehyde (FA) and antibody labelings were using rat anti-BrdU (Abcam, ab6326). Immunofluorescence staining was performed as described before51 ...
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bioRxiv - Genetics 2019Quote: Rat monoclonal anti-KDEL and rabbit monoclonal anti-GM130 antibodies were from Abcam (ab50601 and ab52649, respectively). Rabbit polyclonal anti-TRAPα antibodies were from Novus Biologicals (NBP1-86912) ...
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bioRxiv - Immunology 2021Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:200). Germinal center B cells were labeled using rat anti-GL7 antibody (FITC ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices were incubated with rat anti-BrdU antibody (1:500 ab6326, Abcam in TBS plus II) overnight at 4°C under shaking ...
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bioRxiv - Immunology 2021Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:1000) and Bcl6 antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection was done with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam) and mouse anti ssDNA (clone16-19 ...
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bioRxiv - Molecular Biology 2022Quote: ... overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734, Abcam) in blocking buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... Anti-Germ cell-specific Rabbit Polyclonal DPP3A/Stella (19878) and TRA98 (Ab82527) Rat monoclonal one from Abcam. For Immunofluorescence ...
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bioRxiv - Immunology 2020Quote: ... Cell specific staining for macrophage phenotypic markers was performed using a monoclonal Rat anti-F4/80 (Abcam) or directly conjugated anti-CD169-AF488 or anti-CD68-AF647 (both Biolegend) ...
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bioRxiv - Microbiology 2020Quote: ... rat anti-PfBiP (1:1,000) (62) and rabbit anti-human-haemoglobin-α primary antibodies (1:1,000, Abcam) followed by chemiluminescence detection with horseradish peroxidase-coupled secondary antibodies (1:10,000 ...
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bioRxiv - Bioengineering 2021Quote: ... the blot was incubated with a peroxidase-linked rabbit anti-rat secondary antibody (Ab6250, Abcam, Cambridge, UK) diluted 1:25,000 in TBST with 2% ECL Blocking Agent ...
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bioRxiv - Microbiology 2022Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:1000), GC cells stained using Bcl6 antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:200). HIV-1 vaccine-induced GCs were studied using immunostaining ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated with rat monoclonal anti-Myelin Basic Protein primary antibody (1:400; Abcam - ab7349) in PBST containing 2%(v/v ...
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bioRxiv - Neuroscience 2023Quote: ... and (3) rat anti-BrdU (1:200, Accurate Biochemicals) and rabbit anti-calretinin (1:250, Abcam, USA). Following washes ...
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:200). B cells were labeled using anti-B220 antibody (eBioscience ...
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bioRxiv - Genetics 2023Quote: ... according to manufacturer’s instructions using a recombinant rat monoclonal STAG1 antibody (Abcam ab241544, Lot:GR3334172-1; 1:200) after cooking 10 minutes at 110°C in antigen unmasking solution (Vector #H3301) ...
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bioRxiv - Microbiology 2023Quote: ... followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam, catalog no. ab150165, 1:1000). GC forming cells were stained using Bcl6 antibody (Abcam ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies included: rat anti-E-cadherin (1:250, AB11512, Lot# 1012145-3, Abcam, Waltham, MA, USA), goat anti-Jagged1 (1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... incubation for 1h with Alexa 647 conjugated secondary antibodies of goat against rat IgG (ab 150159, Abcam) or goat anti mouse IgG (A-21235 ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were diluted in blocking buffer and applied overnight: rat anti-BrdU antibody (Abcam cat # Ab6326) was used to detect CldU ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and ALP-conjugated Goat Anti-Mouse IgG Fc (Abcam, ab98710, 1:1000) in 50 μL Blocking Buffer was added ...
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bioRxiv - Immunology 2020Quote: ... 96-well ELISA plates were coated overnight with 1 µg/well of polyclonal rabbit anti-ESAT-6 Ab (#ab45073, Abcam Inc, USA) followed by blocking with 2% BSA for 1 h at 37° C or overnight at 4° C ...
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bioRxiv - Pathology 2021Quote: ... The RBD-specific IgA titer of BAL samples was next evaluated by ELISA as described above with Goat anti-mouse IgA alpha chain-HRP (Abcam, 1:3000).
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bioRxiv - Neuroscience 2021Quote: ... the level of TNF-α was quantified in cell culture supernatant after 72h (end of the culture) by ELISA according to manufacturer instructions (Abcam, #ab46070).
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bioRxiv - Microbiology 2022Quote: The concentration of host α-amylase in swab supernatants was measured using a pancreatic α-amylase sandwich ELISA (Abcam, Toronto, ON, CA). The α-amylase standard (50 µL ...
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bioRxiv - Molecular Biology 2022Quote: ... blood samples from each repopulated mouse were collected via the saphenous vein and analyzed using the Human Albumin ELISA Quantitation Set (Abcam, Cambridge, UK) according to the manufacture’s protocol ...
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bioRxiv - Immunology 2023Quote: ... as described previously.25 IgA levels in lung homogenates were measured by ELISA with goat anti-mouse IgA a chain antibody conjugated to horseradish peroxidase (ab97235; Abcam, Cambridge, UK), as previously reported.10 MPO activity was measured in lung homogenates as reported previously25 ...
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bioRxiv - Immunology 2023Quote: ... anti-ssDNA antibodies and total IgG were assessed by ELISA (Alpha Diagnostic International; 5120, Alpha Diagnostic International; 5320 and Abcam; ab157719 respectively) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... The Alexa Fluor 488 (Donkey Anti-Rabbit and Anti-Rat IgG H&L) were also obtained from Abcam. The APC-alpha-6Itg anti human/mouse CD49f was obtained from BioLegendR ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were stained with rat anti-Sox2 (eBiosciencce) and FITC-conjugated mouse anti-cTnT antibodies (Abcam, Cat#ab105439) (E9.5 only) ...
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CD8+ T-cell-mediated immunoediting influences genomic evolution and immune evasion in murine gliomasbioRxiv - Cancer Biology 2019Quote: ... IHC was performed with a 1:150 diluted rat monoclonal antibody against MHC-I (AB15680) (Abcam; Cambridge, UK), 1:150 diluted rabbit polyclonal antibody against MHC-II (AB180779 ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies were used at the indicated dilution: anti-tubulin (rat monoclonal, Abcam #ab6161; 1:200), anti-phospho-ERM (rabbit monoclonal ...
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CAP1 and cofilin1 cooperate in neuronal actin dynamics, growth cone function and neuron connectivitybioRxiv - Neuroscience 2020Quote: ... 10% donkey serum and 0.5% NP40 in PBS and stained over night at 4°C with following primary antibodies in 2% BSA and 0.5% NP40 in PBS: rat anti-Ctip2 (1:1,000, Abcam), rabbit anti-Neurofilament200 (1:80 ...
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bioRxiv - Microbiology 2021Quote: ... Primary immuno-detection of BrdU was with rat anti BrdU BU1/75 (ICR1) antibody (1:100 dilution, Abcam). Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... 347580)] for 110 minutes at 37°C followed by washing and secondary antibodies goat anti-rat Cy5 (Abcam, ab6565) and goat anti-mouse Cy3.5 (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... rats were tested for their intake following treatment with saline or CCK-8 (4μg/kg IP; Abcam, ab120209) 30 minutes prior to being placed in the chamber ...