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Citations for abcam :
1151 - 1200 of 1298 citations for 7 methoxynaphthalen 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Standard Western Blot analysis using α-B3GLCT (IHC-plus antihuman B3GALTL antibody, 1:500) and α-rabbit-HRP (2°, 1:5,000, Abcam)] was performed 9 ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin immunoprecipitation was performed by incubation of the chromatin fraction overnight with 20 μl of protein-A coated beads (Thermo-Scientific, 53139) and 2 μg of H3K4me1 specific (Abcam, ab8895) or 2 μg of H3K4me3 specific (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were incubated for 2 hours at RT or overnight at 4°C in presence of polyclonal chicken anti-MAP2 (Abcam, ab5392) and polyclonal guinea pig anti-Synaptophysin1 (SySy ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked in 3% skim milk for 2 hours and then incubated with primary antibody overnight at 4°C for the following proteins: 4HNE (Abcam, ab46545), β-actin (Santa Cruz Biotech) ...
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bioRxiv - Genetics 2021Quote: ... Cells were incubated with primary antibody (1:400 RNAPII [pSer2] Novus Biologicals NB100-1805, 1:400 PCNA [5E6/2] abcam ab70472) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Baltimore PKD Center), anti-PC-2 monoclonal antibody (3374 CT-1 414, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein tech 50430-2-AP), anti-RFP (rabbit, 1:3 000, St. Johns Laboratory STJ97083) and anti-H2B (mouse, 1:3 000, Abcam, ab52484). Membranes were washed 3 times for 10 min each in PBST ...
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bioRxiv - Genetics 2022Quote: ... 500 ug lysate was incubated at 4□ overnight with 2 ug of appropriate antibodies (anti-H3K4me3 (Abcam, Cat# ab8580, RRID: AB_306649), anti-H3K36me3 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Floating sections were captured onto gelatin slides in serial order from caudal to rostral brainstem and mounted with 4’6’-diamidino-2-phenylindole dihydrochloride (DAPI) (Abcam, Cambridge, MA).
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times in PBS for 15 min and incubated in secondary antibody for 2 h at room temperature: anti-mouse IgG (1/1,000, HRP-conjugated, Abcam, No. ab205719), anti-guinea pig IgG (H + L ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: We processed hippocampal sections to assess the numbers of transit-amplifying hippocampal progenitors immunopositive for the transcription factor T-box brain protein 2 (rabbit anti-Tbr2, 1:500, Abcam, USA), immature neurons immunopositive for doublecortin (DCX ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Physiology 2023Quote: ... Immunoprecipitation of the cleared lysates was performed overnight at 4C with 5μg of anti-VDR antibody or anti-RXRα monoclonal antibodies (Abcam – Table 2). The beaded complexes were washed several times with NP-40 lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 1% normal goat serum (NGS) for 1 hour and incubated overnight with primary recombinant anti-prolactin receptor antibody [EPR7184(2)] (abcam, ab170935) at 1:50 in 1% BSA ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 1:4000 fold diluted rabbit anti-SARS-CoV-2 N protein-specific IgG (Abcam, Cat.#ab271180) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... blocked with 5% normal goat serum/2% BSA/0.1% Triton-X for 1 hour and incubated with mouse monoclonal PAX7 antibody (abcam, 1:200) and rabbit monoclonal SOX10 antibody (Cell Signaling Technologies ...
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bioRxiv - Genomics 2023Quote: ... Primary antibody incubations were performed overnight at 4°C in PBS-T with 2% NGS plus one or more of the following antibodies: polyclonal rabbit anti-GFP (1:800, Abcam #ab290), monoclonal mouse anti-Abd-B (1:500 ...
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bioRxiv - Genetics 2023Quote: ... followed by a permeabilisation step using 0.3% Triton X-100 for 10 min and then treated with 1% BSA blocking buffer solution for 2 h prior to overnight incubation with monoclonal anti-pNRF2 (1:200; Abcam ab76026) in a humidified chamber at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Western blot analysis was done as described (IHARA AND NISHIWAKI 2007) using anti-NID-1 (2 μg/ml) (KUBOTA et al. 2008) and anti-α-tubulin (DM1A, 1:1000; abcam), anti-GFP (3E6 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated in 50 μL of 2% goat serum in PBS containing mouse monoclonal anti-NR2A antibody (abcam #240884, 1:200) and rabbit monoclonal anti- Na+/K+-ATPase ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were blocked in 10% FCS and then incubated overnight at 4°C with the following primary antibodies diluted in 2% FCS: LGALS1 (Abcam ab138513), RBP1 (Invitrogen PA528713) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 2 hours at RT with the following primary antibodies diluted in BPS: TAF1 (1:1000, rabbit pAb, Abcam, ab188427), TAF4 (3 μg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Specific sIgA antibodies to the SARS-CoV-2 virus were detected with goat anti-mouse IgA alpha-chain + HRP (Abcam®), diluted to 1:1.000 ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The secondary antibody was then washed with 1X PBS three times and the nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole)-containing mountant (Abcam Ab104139). Samples were imaged with a motorized fluorescence microscope (Olympus IX83 ...
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bioRxiv - Genomics 2023Quote: ... CUT&Tag was performed as previously described93 (detailed protocol DOI: dx.doi.org/10.17504/protocols.io.z6hf9b6) using (Abcam catalog no. ab9050, lot GR3386101-2) polyclonal antibody ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with the SARS-CoV-2 Spike Antibody Mouse Mab (40592-MM117, SinoBiological, China) and HRP-conjugated goat anti-mouse IgG (ab6789, abcam, UK). Detection was carried out using an Ultra High Sensitivity ECL Kit (HY-K1005 ...
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bioRxiv - Cell Biology 2023Quote: ... Blocked cells were then incubated for 1 hour at room temperature with primary antibodies anti-Isl1/2 (Abcam ab109517, 1:2000), anti-Chx10 (Santa Cruz Biotechnology sc-365519 ...
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bioRxiv - Cell Biology 2023Quote: ... and the following secondary antibodies in blocking solution for 2 h at RT: Alexa Fluor 488® goat anti-guinea pig IgG H&L (Cat. No. ab150185; Abcam, UK ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was conducted using 2% w/v BSA in 1×PBS for 1 h and incubated with anti-Ki67 antibody (Abcam ab16667) in a 1:200 dilution ...
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bioRxiv - Molecular Biology 2024Quote: ... H-2, sc-398394, 1:1000, Santa Cruz Biotechnology; anti-CPT1α: ab128568, 1:1000, Abcam; anti-ERRα: ab137489, 1:1000, Abcam). Imaging was done using KODAK films or the Amersham Imager 680 equipment.
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked at room temperature for 2 hours in TBTD supplemented with 10% donkey serum and incubated in anti- SOX2 (AbCam, #ab97959) and anti-SOX17 (R&D ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were blocked with 2% BSA-PBS solution for 1 hr at room temperature and incubated with one or more of the following primary antibodies diluted in 2% BSA-PBS at 4 °C for 48∼72 hr: rabbit anti-Collagen VI (1:200; Abcam, ab182744), rabbit anti-fibronectin (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 hours at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (dilution 1:5000, Abcam #ab6789). After washing again ...
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bioRxiv - Immunology 2024Quote: ... sections were repaired using EDTA antigen recovery buffer and incubated overnight at 4 °C with anti-dengue virus 1+2+3+4 primary antibody (Abcam, ab26837). Next ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated for 2 h at RT with the following fluorophore-conjugated secondary antibodies: goat anti-rabbit Alexa 488 (1:300; Abcam; ab150113), donkey anti-rabbit Cy5 (1:300 ...
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bioRxiv - Immunology 2024Quote: ... chromatin from approximately 106 cells was incubated overnight at 4°C with 2 μg of anti-ERRα or anti-PGC-1α (Abcam, USA) ChIP-grade antibody and 20 μL of A/G magnetic beads ...
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bioRxiv - Cell Biology 2024Quote: ... The cell extract was then incubated with magnetic beads conjugated with anti-Argonaute 2 (AGO2) or anti-IgG antibody (Abcam, USA) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were washed three times in PBS containing 0.01% Triton X-100 and incubated for 2 hours with AlexaFluor conjugated donkey anti-rabbit secondary antibody (Abcam ab150073 1:1000). After secondary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... the same ACE2 IHC protocol was applied to other 2 primary antibodies anti-Human ACE2: monoclonal rabbit anti-Human ACE2 (cat. Ab108252, Abcam, Cambridge, UK) diluted 1:100 in PBS 1× supplemented with 5% goat serum ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were rotated for 2 hours at 4°C in 200 μL DigWash with 1:400 Lamin B1 antibody (Abcam, ab16048, rabbit), followed by a wash step with DigWash ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was then washed thrice with 1X-TBST and incubated with anti-SARS-CoV-2 N protein antibodies (catalogue No. ab272852, Abcam, 1:1000) in TBST with BSA (2% ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: Fixed hUC-MSCs were incubated with antibodies against nuclear proteins such as CENP-A (1:100 in PBS; o/n at 4°C + 2 hr at 37°C) (Abcam; ab45694, USA), lamin A/C (1:100 in PBS ...