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Citations for abcam :
1151 - 1200 of 10000+ citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 1h in blocking buffer (PBS, 10 % FBS, 1 % Saponin) followed by primary antibody incubation overnight at 4°C (Calnexin, Abcam ab22595 at a dilution of 1:200). After washing twice with wash buffer (PBS ...
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bioRxiv - Microbiology 2019Quote: ... Ultra-thin sections were blocked with 5% fetal bovine serum and 5% normal goat serum in 1× PBS for 30 min and subsequently stained with rabbit anti-GFP antibody (Abcam, 1:500 dilution in blocking buffer), washed thoroughly and incubated with 18 nm colloidal gold-conjugated anti-rabbit IgG for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... ISLET-1 (1/200; Abcam), HB9 (1/10 ...
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bioRxiv - Molecular Biology 2023Quote: ... HO-1 (Abcam, 1:2000), TOMM20 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... The ELISA plates were washed 4 times by Blocking Buffer and 50 μL Goat F(ab’)2 Anti-Human (IgG (Fab’)2) secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Reacted mAbs were detected using ALP-conjugated Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) for 30 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The ELISA plates were washed 4 times by blocking buffer and 50 μL Goat F(ab’)2 Anti-Human IgG (Fab’)2 secondary antibody conjugated with ALP (Abcam, ab98532, 1:2000) was incubated with the plate at RT for 30 mins ...
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bioRxiv - Neuroscience 2021Quote: Pool of 2-3 MOs were lysed in RIPA lysis buffer (Abcam, cat no. ab156034) containing 1X protease inhibitor cocktail (PIC)(Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Abcam ab211091, Cambridge, UK) was used to confirm treatments did not affect cell viability ...
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bioRxiv - Cell Biology 2024Quote: ... and Heme Oxygenase 1 (HO-1) (1:200; ab13248; Abcam), and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... coli were purchased and added to Kinase Buffer I (25 mM MOPS pH7.2, 12.5 mM β-glycerol phosphate, 25 mM MgCl2, 5 mM EGTA, 2 mM EDTA, 0.25 mM DTT; Abcam ab189135). Either water (mock ...
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bioRxiv - Bioengineering 2021Quote: ... and blocked for 2 hours in 5% normal goat serum (NGS, Abcam, ab7481) in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... and blocked for 2 hours in 5% normal goat serum (NGS, Abcam, ab7481) in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... and blocked for 2 hours in 5% normal goat serum (NGS, Abcam, ab7481) in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... and blocked for 2 hours in 5% normal goat serum (NGS, Abcam, ab7481) in PBS ...
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bioRxiv - Pathology 2022Quote: ... We blocked tissue with 5% goat serum (2% for 1D1; Abcam, Cat# AB7481) in PB for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... D-(-)-2-amino-5-phophonopentanoic acid (D-AP5; 50μM; Abcam Biochemicals, catalog #120003), 1-(4-aminophenyl)-3-methylcarbamyl-4-methyl-3,4-dihydro-7,8-meth-ylenedioxy-5H-2,3-benzodiazepine hydrochloride [GYKI-53655 (GYKI) ...
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bioRxiv - Neuroscience 2021Quote: ... and 50 μM DL-2-Amino-5-phosphonopentanoic acid (Abcam, Cambridge, UK, ab120044). NH4Cl alkaline buffer (50 mM NH4Cl substituted for 50 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 μM DL-2-Amino-5-phosphonopentanoic acid (Abcam, Cambridge, UK, ab120044). Alkaline buffer (50 mM NH4Cl substituted for 50 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... followed by staining after 2 or 4 weeks using Senescence detection kit (Abcam, ab655351). Photos were taken using an upright microscope at 20X magnification.
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bioRxiv - Cell Biology 2022Quote: ... nuclei were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI; Abcam, Waltham, MA, USA). Similarly ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were counterstained with 4′,6′-diamidino-2-phenylindole (DAPI; Abcam, Waltham, MA, USA). Similarly ...
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bioRxiv - Pathology 2024Quote: ... and mounted using Fluoroshield Mounting Medium with 4′,6-diamidino-2-phenylindole (ab10413; Abcam). PBS served as the negative control ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Slides were subsequently blocked with blocking buffer (3% natural serum, 1% BSA, 0.1% Tween-20) and incubated overnight with anti-EZH2 antibody (3748; Abcam), anti-CREB3L1 (AF4080 ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970 ...
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bioRxiv - Immunology 2019Quote: ... unspecific binding sites were blocked with 3% BSA and cells were incubated with primary anti-myeloperoxidase (1:1000, Abcam), anti-elastase (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight in 3% normal donkey serum and 0.3% PBST containing primary antibodies for GFP (1:2000, chicken anti-GFP, ab13970, Abcam), and/or TH (1:2000 ...
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bioRxiv - Biophysics 2024Quote: ... Attached cells were then fixed by methanol/acetic acid solution (3:1) stained with Perl’s Prussian blue with nuclear fast red as counterstain (Iron stain kit, ab150674, abcam). Slides were dehydrated (incubation with 75 ...
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bioRxiv - Microbiology 2022Quote: ... then incubated for 1 h at room temperature in 20 mL TBS-T with 3% milk powder and 1:5,000 diluted anti-GFP antiserum (Abcam) while shaking with 180 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... the sectioned slices were incubated for one hour at room temperature in blocking solution (10% normal goat serum and 0.3% Titron X-100 in 1x TBS) and incubated overnight in polyclonal rabbit anti-HA (1:1,000, #ab9110, Abcam) at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight in primary antibodies then washed (3 x 0.1M PBS) and incubated in secondary antibodies conjugated to Alexa-594 (1:200, Abcam) or Alexa-488 (1:200 ...
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bioRxiv - Genetics 2019Quote: ... Samples were incubated overnight at 4°C with 2.5-5 μg anti-TBP (58C9, Abcam, or 5 μL serum ...
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bioRxiv - Cell Biology 2019Quote: ... the gels were covered with 2 mg mL-1 Sulfo-SANPAH (no. ab145610, abcam, Cambridge, UK) and activated by ultraviolet light for 5 min with an Ultraviolet Crosslinker CL-1000 apparatus (UVP ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were incubated with 1:500 anti-SARS-CoV-2 Spike glycoprotein antibody (Abcam, Catalog#ab272504) diluted in blocking buffer for overnight at 4 degree ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:100 or TrkB (kinase specific against amino acid 810; abcam #ab18987, lot #GR3280550-2) at 1:250 ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were first incubated with anti-Nrf-2 antibody (Abcam, Ab-62352, 1:250 dilution) at 4°C overnight and then with Alexa Flour secondary antibody (Pierce ...
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bioRxiv - Cancer Biology 2021Quote: ... A Rabbit polyclonal antibody to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) (Abcam) was also used at 1:1000 dilution for 1h at room temperature as well as the respective peroxidase conjugated goat anti-Rabbit IgG secondary antibody (ThermoFisherScientific ...
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bioRxiv - Bioengineering 2021Quote: ... and with 2% BSA in PBS + 0.05% Tween-20 for anti-FoxO3 (Abcam, ab109629, 1:1000) and anti-RAPTOR (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5% BSA and 2 mM EDTA added to Anti-GFP antibody (1:500, Abcam, Ref. ab6556) for 15min at 4-8°C ...
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bioRxiv - Biochemistry 2023Quote: ... at room temperature and incubated with 1:1000 rabbit anti-LRRK2 monoclonal antibody (Abcam c41-2) diluted in 1% BSA O/N at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Biotin-conjugated goat anti-human IgG (Fab’)2 antibody (Abcam, Waltham, MA, USA; 1 μg/ml) was subsequently added for 1 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... or blocked in 5%NGS/PBST for 1hr and incubated overnight with THSB4 (Abcam, ab211143, 1:100) and α-Myosin Heavy Chain (F59 ...
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bioRxiv - Physiology 2022Quote: ... dantrolene (1 µM from a stock concentration of 5 mM in DMSO, to stabilise ryanodine receptors, Abcam), streptomycin (50 µM from a stock concentration of 15 mM in distilled water ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then incubated overnight with antibodies in 5% BSA TBS-Tween: 1:1000 PKCζ (Abcam, ab59364), 1:10,000 GAPDH (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... 5% donkey serum and 1% BSA in PBS and immunostained (Primary antibody: chicken-anti-GFP, ab13970, Abcam, 1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... Secondary antibody (goat-anti rabbit HRP 1:10000 in 5% BSA) was purchased from Abcam (Cambridge, UK). Bands were quantified using LICOR C-Digit chemiluminescent detection system (LICOR Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... or goat anti-CHRM1 antibody (1 µg/ ml, diluted in 5% BSA in 0.1% TBST) (Abcam ab77098) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... PVDF membranes were blocked in 5% milk-PBST and then probed with NRAS (1:250, Abcam ab77392) and β-Actin (CST 3700S ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated with the primary antibody diluted in PBS containing 0.5% Tween-20 and 5% NGS (rabbit anti-mCherry antibody, Abcam, ab167453, RRID:AB_2571870, 1:5,000; chicken anti-mCherry antibody, Abcam, ab205402 ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated in blocking buffer for 2 h at room temperature (rabbit anti-ACE2 [Abcam #ab15348, used at 1:500 dilution]; mouse anti-GAPDH [Abcam #ab8245, used at 1:3,000 dilution]). Membranes were washed and further incubated with fluorescent dye-conjugated secondary antibodies for 1 h at room temperature (AlexaFluor 647 goat anti-rabbit IgG1 and AlexaFluor 488 goat anti-mouse IgG1 ...