3 1 3 Dioxan 2 yl 3' methoxypropiophenone citations for abcam

Quote: ... and labelled with primary antibody (1:1000 diluted in blocking buffer made up by 1% non-fat dry milk in calcium and magnesium free PBS) to tyrosinated tubulin [YL1/2] (rat monoclonal, ab6160, Abcam) for 45 min at room temperature which is followed by PBS washes repeated three times and 10 min of blocking ...

Circulating levels of epirubicin cause endothelial senescence while compromising metabolic activity and vascular function

Amanda J Eakin, et al., bioRxiv - Cell Biology 2020

Quote: ... Slides were washed 3 times in PBS and then incubated for 2 h in secondary antibody (1:200 dilution AlexaFluor647 conjugated donkey anti-goat (ab150135, Abcam) and 1:200 dilution FITC-conjugated rabbit anti-rat (ab6730 ...

The secretome of M1 and M2 microglia differently regulate proliferation, differentiation and survival of adult neural stem/progenitor cell

Xue Jiang, Saini Yi, Qin Liu, Jinqiang Zhang, bioRxiv - Neuroscience 2021

Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68 ...

Behavioral and molecular underpinnings of performance variability in eyeblink conditioning in male and female mice

Maria Roa Oyaga, et al., bioRxiv - Neuroscience 2021

Quote: ... sections were incubated for 48 hours at 4°C on a shaker in primary antibody solution with 2% NHS (1:2000 Rabbit anti-C-FOS, ab208942, Abcam; 1:1000 Rat anti-Ctip2 ...

CMTr cap-adjacent 2’-O-ribose mRNA methyltransferases are required for reward learning and mRNA localization to synapses

Irmgard U. Haussmann, et al., bioRxiv - Molecular Biology 2021

Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10 ...

The small GTPase Arf1 regulates ATP synthesis and mitochondria homeostasis by modulating fatty acid metabolism

Ludovic Enkler, et al., bioRxiv - Cell Biology 2022

Quote: ... Sections were blocked with PBST (PBS+ 0.05% Tween 20) + 2% BSA for 15 min and incubated 3h at RT with anti-GFP antibody (1:100, 6556 Abcam) in PBST/BSA ...

Preconditioning by voluntary wheel running attenuates later neuropathic pain via Nrf2 antioxidant signaling in rats

Suzanne M. Green-Fulgham, et al., bioRxiv - Neuroscience 2021

Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...

Protective anti-prion antibodies in human immunoglobulin repertoires

Assunta Senatore, et al., bioRxiv - Neuroscience 2020

Quote: ... sections were blocked and incubated with the anti-PrP Fabs at 12 µg/ml and antibody for Microtubule Associated Protein 2 (MAP2) (1∶500, Abcam). Double staining was performed with fluorescently-labeled secondary antibodies (goat anti Human IgG F(ab’)2:Tritc 1:150 ...

VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms

Jim P. Fouracre, et al., bioRxiv - Plant Biology 2021

Quote: ... 2% was removed as input and samples were incubated overnight with 1% antibody (for histone ChIP: anti-H3 (abcam ab1791), anti-H3K27me3 (EMD Millipore 07-449) ...

Cardiac Microlesions Form During Severe Bacteremic Enterococcus faecalis Infection

Armand O. Brown, et al., bioRxiv - Microbiology 2020

Quote: ... paraffin embedded sections were treated with 1:100 rabbit Anti-mouse MLKL (phospho S345) antibody [EPR9515(2)] in order to detect phosphorylated MLKL (Abcam), and 1:100 rat anti-mouse MLKL antibody (Millipore-Sigma ...

Dysfunctions of the paraventricular hypothalamic nucleus induce hypersomnia in human and mice

Chang-Rui Chen, et al., bioRxiv - Neuroscience 2021

Quote: ... Primary antibodies were washed five times with PBS before incubation with secondary antibodies at room temperature for 2 h (Alexa 488, 1:1000; abcam). Finally ...

Glycomic analysis of host-response reveals high mannose as a key mediator of influenza severity

Daniel W. Heindel, et al., bioRxiv - Microbiology 2020

Quote: ... cells were stained with mouse monoclonal anti-MBL (Biotin) (2 μg/ml in MBL2 wash buffer; 1 h, 37 °C; Abcam) and mouse monoclonal anti-influenza A that had been labeled with Cy3 dye and dialyzed following the manufacturers protocol (1 μg/ml in MBL2 wash buffer ...

Got Milk? Maternal immune activation during the mid-lactational period affects nutritional milk quality and adolescent offspring sensory processing in male and female rats

Holly DeRosa, Hieu Tran, Amanda C. Kentner, bioRxiv - Neuroscience 2022

Quote: ... Undiluted milk samples and blood plasma (diluted 1:2) were processed using the LPS binding protein ELISA (Abcam, Cat. #ab269542). Manufacturer’s instructions were followed for all ELISA kits (n = 7).

Chronic Seizures Induce Sex-Specific Cognitive Deficits with Loss of Presenilin 2 Function

Kevin M. Knox, et al., bioRxiv - Neuroscience 2022

Quote: Slides were similarly processed for BDNF with the exception that slides were blocked with 10% sheep serum in 5% Triton-X PBS under coverwells in a humid chamber for 2 hours and slides were incubated with primary antibodies for BDNF (1:500; ab226843, Abcam) and NeuN (1:300 ...

SARS-CoV-2 infected cells sprout actin-rich filopodia that facilitate viral invasion

Yue Zhang, et al., bioRxiv - Cell Biology 2022

Quote: ... The following antibodies were used in this study: anti-SARS-CoV-2 spike glycoprotein rabbit polyclonal antibody (dilution 1:1000; #ab272420, Abcam); anti-SARS-CoV-2 nucleocapsid protein rabbit polyclonal antibody (dilution 1:1000 ...

α-Synuclein-112 impairs synaptic vesicle recycling consistent with its enhanced membrane binding properties

Lindsey G. Soll, et al., bioRxiv - Neuroscience 2020

Quote: ... the membranes were incubated for 2 hrs with a rabbit polyclonal pan-synuclein antibody (1:1000; ab53726; Abcam, Cambridge, MA). After washing in TBST ...

Interactions between RNA m⁶A modification, alternative splicing, and poly(A) tail revealed by MePAIso-seq2

Yusheng Liu, et al., bioRxiv - Molecular Biology 2021

Quote: ... Total RNA samples were subjected to immunoprecipitation with m6A specific antibody (m6A-1, Active Motif, #91261; m6A-2, Abcam, #ab151230) as previously described5 ...

SARS-CoV2, a threat to marine mammals? A study from Italian seawaters

Tania Audino, et al., bioRxiv - Pathology 2021

Quote: ... for 20 min before being incubated overnight with the primary anti-ACE-2 antibody (a polyclonal antibody raised in rabbits and diluted 1:2000, ab15348 Abcam) at 4°C in Tris-Buffered Saline (TBS ...

Abi3 regulates microglial ramification and dynamic tissue surveillance in vivo

Elena Simonazzi, et al., bioRxiv - Neuroscience 2021

Quote: ... Sections were washed 3 times with 1x TBS and incubated for 2 hours at room temperature with appropriate secondary antibodies (goat anti-chicken FITC, 1:200, Abcam or goat anti-rat AF488 ...

DNA methylation across the genome in aged human skeletal muscle tissue and stem cells: The role of HOX genes and physical activity

DC Turner, et al., bioRxiv - Physiology 2020

Quote: ... in 300 μl of TBS (with 2% goat serum and 0.2% Triton X-100) containing primary anti-desmin antibody (1:50; ab15200, Abcam, UK). After overnight incubation ...

Labshake search