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Citations for abcam :
9951 - 10000 of 10000+ citations for IRF2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then incubated with primary antibodies for 1 hour at 37 °C (1:250 mouse anti-MSH2 Abcam ab52266; 1:500 rabbit anti-PARP1 Abcam ab227244 ...
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bioRxiv - Molecular Biology 2024Quote: ... overnight incubation at 4°C with both mouse anti-NR5A1 (1:200, #sc-393592, Santa-Cruz Biotechnologies) and rabbit anti-NR2F2 (1:200, #ab42672, Abcam) primary antibodies was performed ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight at 4⁰C on a plate rocker in a 5% NGS/0.1% PBS-Triton solution containing rabbit anti-cFos antibody (Abcam, ab190289) at 1:500 dilution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and stained with a rabbit monoclonal antibody against β1 integrin and a mouse monoclonal antibody against PCNA (Abcam, Cambridge, UK) overnight at 4℃ ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times in PBS then incubated with Alexa 488-conjugated goat anti-rabbit secondary antibody (1:500; ab150077, Abcam) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed in PBS and a solution of fluorophore-conjugated secondary antibodies (1:300 horse anti-rabbit 594, Vector, Olean, NY, Cat#DI-1094 and 1:200 goat-anti guinea pig 488, Abcam, Cambridge ...
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bioRxiv - Physiology 2024Quote: ... Tissues were then incubated in a primary antibody solution (1:500 rabbit anti-serotonin, Immunostar, and 1:500 goat anti-collagen IV, abcam) for two days at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then washed with Tris-Buffered Saline with Tween-20 and incubated in one of the following secondary antibodies: Goat Anti-Rabbit IgG H&L (HRP) (Abcam, ab6721 ...
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bioRxiv - Neuroscience 2024Quote: ... co-staining IBA-1 – ZO-1 incubation during 24 h at room temperature (1:1000, ab178846, rabbit anti-IBA-1, Abcam; 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...
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bioRxiv - Genetics 2024Quote: ... slides were washed 3 times with PBS and incubated with isotype-specific anti-rabbit secondary antibody labeled with Alexa Fluor 488 (1:500, Abcam) and anti-mouse secondary antibody labeled with Alexa Fluor 568 (1:500 ...
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bioRxiv - Immunology 2024Quote: ... Secondary antibodies used were an anti-Syrian hamster coupled to AF647 (Stratech) and an anti-rabbit coupled to AF555 (Abcam). Multiplex immunofluorescence imaging was performed on FFPE sections using an Opal multiplex IHC kit (Akoya Biosciences) ...
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bioRxiv - Immunology 2024Quote: ... the BD Cytofix/Cytoperm kit was used to permeabilize the cells and the cells stained with the following unconjugated rabbit anti-adenosine receptor antibodies from Abcam as previously described (21) ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then permeabilized in 1mL of 0.1% Triton X-100 in 1X PBS for 5 min at room temperature and incubated with anti-DDX5 primary antibody (rabbit, ab21696; Abcam) diluted to 1:400 in PBS 1X for 1 hour ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Cancer Biology 2023Quote: ... Matched lung tumor tissues from chemoresistant and chemosensitive patients (as determined in PDTOs) were immunostained with anti-AKR1B10 antibody (rabbit, ab192865; 1:1000; Abcam) and detected by DAB method.
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies used for double staining were either the combination of Alexa Fluor 594-conjugated anti-rabbit IgG antibody (ab150080, abcam) and Alexa Fluor 647-conjugated anti-mouse IgG antibody (a21237 ...
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bioRxiv - Genomics 2023Quote: ... We stained polytene chromosomes with rabbit anti-CLAMP (1:1000, SDIX) and mouse anti-RNA pol II (1:500, ab817, Abcam) antibodies ...
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bioRxiv - Developmental Biology 2023Quote: The following antibodies were used for chromatin immunoprecipitation: rabbit anti-NK Homeobox 2-1 (NKX2-1, 1 µg per reaction, ab133737, Abcam) and rabbit anti-CEBPA (C/EBPα ...
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bioRxiv - Immunology 2023Quote: ... the specimens were incubated overnight at 4 °C with 5µg/mL rabbit anti-GFP antibody (ab290, Abcam, Cambridge, MA, USA). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... was used at 1:3500 dilution for imaging on the Li-Cor Odyssey detection system and goat anti-rabbit HRP (Abcam) was used for X-ray detection ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed three times with PBST and incubated with primary antibodies diluted in blocking solution: 1:500 Rabbit anti-Iba1 (ab178846, Abcam) and 1:250 chicken anti-GFP (A10262 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-CypA (1:1000) (Enzo) or anti-CypB (1:2000) (Abcam) antibody ...
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bioRxiv - Neuroscience 2023Quote: ... anti-rabbit NOX4 (1:500; Novus Cat# NB110-58851B, RRID:AB_1217375 and anti-mouse GAPDH (1: 10.000; Abcam Cat# ab8245, RRID:AB_2107448). Incubations were performed at 4_C overnight in 5% non-fat milk or BSA.
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bioRxiv - Neuroscience 2022Quote: ... Protein A (if the antibody host was rabbit) or Protein G (if the antibody host was mouse) agarose beads (Abcam) were washed three times in aCSF and centrifuged at 400 g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Mock immunodepletion was performed on the AD and healthy control brain homogenates with normal rabbit or mouse IgG antibodies (Abcam).
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bioRxiv - Microbiology 2022Quote: ... blocked for 1 h using 5% donkey serum and then incubated with a rabbit monoclonal H3Cit antibody (citrulline R2 + R8 + R17, clone EPR20358-120; Abcam) at 1:500 dilution overnight in a humidified chamber at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The two fractions were split to two for IgG and YTHDF1 IP (2 μ each, rabbit IgG isotype control, Abcam ab37415 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’- diaminobenzidine (DAB) were done using the Mouse and Rabbit Specific HRP/DAB IHC Detection Kit - Micro-polymer (ab236466, Abcam). Before the protein block ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated overnight at 4°C with AF647 conjugated rabbit anti-METTL3 primary antibody (ab217109; Abcam; 1:100 dilution), AF488 conjugated rabbit anti-β-IV-Tubulin (ab204003 ...
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bioRxiv - Immunology 2022Quote: a-Smooth muscle actin (aSMA) staining and muscularized vessel count: formalin-fixed lung section was stained for rabbit polyclonal aSMA (Abcam #ab5694 ...
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bioRxiv - Molecular Biology 2023Quote: ... or Dynabeads M-280 Sheep Anti-rabbit IgG mixed with anti-anti-Rpb1 (39) or anti-Histone H3 (ab1791; Abcam) antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (rabbit anti-ac4C, #ab252215, Abcam; rabbit anti-NOP58, #14409-1-AP, Proteintech; mouse anti-NCL, #87792, CellSignal; mouse anti-G3BP, #ab56574, Abcam) were diluted 1:50 in blocking solution and incubated with the samples for 1 hr at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were incubated overnight at 4 °C with the following primary antibodies: anti-METTL3 rabbit antibody (1:300, ab195352; Abcam), anti-METTL14 rabbit antibody (1:300 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were incubated with the blots overnight at 4 °C in blocking solution: anti-myc-tag (1:5,000, rabbit pAb, ab9106, Abcam, UK), anti-Dclk1 (1:1,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated with the secondary antibody (AlexaFluor568-conjugated donkey-anti goat ab, 1:400, and AlexaFluor647-conjugated rabbit-anti mouse ab, 1:2000; both Abcam) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 5% skim milk for 2 h at room temperature then incubated with primary antibodies (Rabbit monoclonal to ATPB at 1/50000 dilution, Abcam) for 16 h at 4 ℃ ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100, cloneC31.7, Abcam, #ab215753) in PTwH/5% DMSO/3% Donkey Serum (Abcam ...
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... The plates were washed four times with PBST and were then incubated with 1:15,000 rabbit polyclonal 6x His-tag antibody conjugated to HRP (Abcam, Austria) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then rinsed in PBS for 20 min before being placed in a blocking solution with secondary antibody used to visualize DAT neurons in the VTA (goat anti-rabbit Alexa Fluor 594, 1:1000 dilution, Abcam, catalog no ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the membrane was incubated with the rabbit anti-IMPA1 antibody (1:10000 diluted in 5% BSA in 0.1% TBST) (Abcam ab184165) or goat anti-CHRM1 antibody (1 µg/ ml ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were incubated overnight at 4°C in a staining solution containing rabbit anti-SOX10 (1:100, Abcam, ab227680).The next day ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody staining was done sequentially in PBS/1% BSA at RT: rabbit anti-GFP (1:500, 1 h, Abcam, 6556), goat anti-rabbit IgG conjugated to 18 nm gold (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... Detroit562 immunoblots were developed using Licor Odessey using secondary antibodies at 1:7,500 - Goat anti-rabbit IgG H&L (IRDye 800CW) and goat anti-mouse IgG H&L (IRDye 680RD) from abcam. Band intensity was quantified by Image Lab (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... 2 µg of rabbit anti-CCT3 (Ray Biotech, 144-06547-200) or 2 µg IgG isotype control antibody (Abcam, ab172730) and incubated at 4°C for 30 min with rotation ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 0.3% Triton X-100 and 90%PBS (Blocking Buffer) for 1 hr at room temperature and then incubated in primary antibodies (rabbit anti-Iba1, Abcam, ab178847 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies (all used at 1:1000) were as follows: donkey anti-rabbit conjugated to Alexa Fluor 594 (Abcam, ab150072), goat anti-rat conjugated to Alexa Fluor 594 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... pT320-pp1 (1:1000 for the detection of the endogenous protein, 1:30000 for the kinase assay, rabbit, Abcam ab62334), pY15-Cdk1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The equivalent of 3-6 x107 cells were precipitated overnight with 1 ug IgG/106 cells of rabbit antibody against H3K27ac (ab4729, Abcam). Before antibody precipitation ...