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Citations for abcam :
951 - 1000 of 1553 citations for 4 4' Bi 7H benz de anthracene 7 7' dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) for 1 h then incubated with the appropriate diluted primary antibody at 4°C overnight: LC3 (Abcam, ab48394) 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C for 24 hours in 2% normal goat serum together with rabbit anti-Nrf2 at 1:500 (Abcam). Slides were then washed and incubated in the secondary antibody (1:1500 ...
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bioRxiv - Neuroscience 2020Quote: ... the cultures were fixed for 1h at room temperature in 4% paraformaldehyde and stained with mouse anti-neurofilament (RMO 270; Zymed) and rabbit anti-myc antibodies (Abcam). The number of neurofilament-positive cells was counted in 16 randomly selected frames (0.4 mm2) ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were blocked with 5 % normal goat serum in Tris B for 30 minutes and incubated overnight at 4 °C with a rabbit polyclonal primary antibody against mCherry (1:3000, #167453, Abcam) diluted in blocking solution ...
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... tissue was incubated with primary antibodies overnight at 4°C at the following dilutions: goat anti-IBA1 1:500 (ab178846, Abcam), rabbit anti-DAAM2 1:50 (HPA054630 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at room temperature followed by overnight incubation at 4°C with primary antibodies: 0.6 μg/mL polyclonal rabbit anti-SFPQ (ab38148, Abcam, UK), and 1:5000 monoclonal mouse anti-GAPDH (60004-1-Ig ...
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bioRxiv - Bioengineering 2021Quote: ... or H3K9me3 antibody for 30 minutes with rotation at 4°C in 100 μL of incubation buffer (ab1220 1:200, ab9045 1:200, ab8898 1:250, Abcam). Incubation buffers tested were 0.1% PBSA ...
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bioRxiv - Bioengineering 2021Quote: ... 5 x 106 beads were then incubated with an H3K9me2 antibody for 30 minutes with rotation at 4°C in 100 μL of 1% PBSA (ab1220 1:200, Abcam). Samples were then washed with 1% PBSA and incubated with secondary antibody for 10 minutes with rotation at 4°C in the dark in 100μL 0.1% PBSA (ab150107 1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... blocked for 1 hr and incubated overnight at 4 °C in secondary antibodies conjugated to Alexa fluorophores (donkey anti-goat, 1:1000, Abcam).
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bioRxiv - Neuroscience 2021Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in blocking solution (goat anti-ChAT, 1:200, Abcam). Fish were then washed in PBT ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation for 30 min at room temperature and/or incubation overnight at 4 °C with primary antibodies (Rabbit anti-NKCC1: 59791, Abcam, 1:400 in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2, BD Bioscience; ARL13B, ProteinTech; IMPDH2, Abcam; SMO ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C for antibody crosslinking (anti-HA, ProteinTech; Normal Rabbit IgG, Cell Signalling; ARL13B, ProteinTech; and IMPDH2, Abcam) at a dilution of 1:66 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated overnight at 4°C with one of the following primary antibodies: GFP (chicken, 1:2000, Abcam, #ab290), GFAP (rabbit ...
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bioRxiv - Biochemistry 2021Quote: ... The immunoprecipitation of HA-tag proteins was performed overnight in a total volume of 600 μl with 4 μg of HA-antibody (ab9110, Abcam) except the 5% of samples taken as input ...
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bioRxiv - Bioengineering 2021Quote: ... The membrane was incubated with primary antibody overnight at 4°C with collagen I (1:1000 dilution, ab34710, Abcam, Canada), tenomodulin (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific binding was blocked by incubating the membranes with 5% nonfat milk for 2 h at room temperature and the identity of proteins evaluated by incubation at 4 ℃ overnight with the following primary antibodies (Abcam): HSP70 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 500 µg of total protein extract were used to IP with either 2.5 µg V5 rabbit polyclonal antibody or VPS35 mouse monoclonal over night at 4°C and recovered after incubating for 6h at 4°C in a rotator with equilibrated Agarose Protein-G beads (Abcam). Immunoprecipitation followed by MS analysis was performed as described but using magnetic Protein G beads (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... The following day the slices were washed three times for 10 minutes in PBS and incubated with secondary antibodies overnight at 4°C (Goat-anti-Guinea-pig-Alexa Fluor 488, Abcam ab150185 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were then incubated overnight at 4°C with the primary antibodies (goat anti-GFP; Abcam AB5450, lot GR3319697, dilution 1:1000; rabbit anti-NeuN, Abcam AB104225 ...
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bioRxiv - Genomics 2020Quote: ... The sonicated chromatin had a size in the range of 100–500 bp and was incubated overnight at 4°C with the anti-H3K27ac antibody (#ab4729, Abcam). Samples were incubated for 1h at 4°C with Dynabeads Protein G for Immunoprecipitation (#10003D ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 min at RT and then overnight at 4 °C in a humidified chamber with the following antibodies: LC3 (1:50, Abcam), p62 (1:20 ...
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bioRxiv - Cell Biology 2021Quote: ... The sections were blocked in normal goat serum for 30 minutes and incubated overnight at 4°C with the primary antibodies against TCF1 (Ab96777, Abcam; 10 µg/ml for knee ...
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bioRxiv - Cell Biology 2021Quote: ... at room temperature for 30 minutes prior to incubation at 4°C overnight with primary antibodies [myosin VIIa (inner HC marker) 1:100 dilution (rabbit polyclonal-ab3481, ABCAM); prestin (outer HC marker ...
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bioRxiv - Cancer Biology 2020Quote: Cytosol and mitochondria were separated from A375 cells 2 hr or 4 hr after treatment using the Mitochondria/Cytosol Fractionation Kit (Abcam) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: 10 µg of protein from 4-week-old adrenals were used for measurement with PKA kinase activity kit (ab139435, Abcam).
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bioRxiv - Cancer Biology 2022Quote: ... and incubated for 16h in 4°C with one of the following antibodies: anti-Collagen VI (1:1000; ab182744, Abcam), anti-YAP (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... paraffin slices were blocked for 1 hour at 37°C with 5% BSA before being treated overnight at 4°C with various primary antibodies as follows: anti-GFAP (Abcam), anti-CD68 (Abcam) ...
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bioRxiv - Neuroscience 2022Quote: ... 0.3% Triton X-100 in PBS) for 1 h and incubated overnight at 4°C with rat anti-BrdU (1:300, Abcam) antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C in a solution containing the Recombinant Anti-Iba1 antibody rabbit (ab178847, 1:500, abcam). After additional washes ...
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bioRxiv - Neuroscience 2022Quote: ... with 0.2% Triton X-100 supplemented with 10% FBS and then incubated overnight at 4 °C with primary antibodies (1:100 mouse anti-ASCL1, BD 556604; 1:800 rat anti-BrdU, Abcam ab6326 ...
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin fractions of 400 μl were taken for each immunoprecipitation reaction and incubated with 4 μl of anti-histone H3 antibodies (ab1791, Abcam) at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... with PBST and stained for primary antibody at 1:200 in PBST for 1 hour at room temperature: mouse anti-alpha sarcomeric actinin (ab9465, Lot: GR3174517-4, Abcam), mouse anti-alpha smooth muscle actin ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining soluble chromatin was incubated overnight at 4°C with 3μg of either c-Myc antibody (anti-c-Myc, Abcam, ab56) or mouse IgG antibody (normal mouse IgG ...
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bioRxiv - Developmental Biology 2022Quote: ... 100µg of cleared chromatin (protein) was incubated with 2µg of the following antibodies overnight at 4°C: H3K27ac (Abcam, ab4729), H3K4me1 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... and then overnight at 4°C in a 1:100 primary antibody solution of the anti-GLUT-1 capillary marker antibody (ab15309, Abcam) and the BBB integrity protein occludin anti-Occludin antibody (ab31721 ...
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bioRxiv - Biophysics 2020Quote: ... then incubated to 37°C for 30 min in the dark with 4 µg/ml of Pyronin Y (abcam, ab146350), diluted in FACS buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were incubated overnight at 4°C with isotype controls and primary antibodies against human CXCL5 and human CXCL8 (Abcam), then detected with biotinylated secondary antibodies (Vector ...
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bioRxiv - Neuroscience 2020Quote: ... the same solution containing the primary antibody overnight at 4°C (anti-tRFP, 1:1000, Evrogen; anti-GABA, 1:2000, MilliporeSigma anti-parvalbumin, 1:2000, Abcam; anti-somatostatin ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibody incubation was overnight at 4°C (anti-RIF1, Bethyl Laboratories A300-568A; anti-MCM4, Abcam ab4459; anti-GFP, Abcam ab290 ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated at 4°C overnight in goat anti-GFP primary antibody (1:2500, Abcam Cat# AB6673, RRID: AB_305643) followed by a four-hour incubation period in donkey anti-goat Alexa Fluor 488 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed and incubated for 72 h at 4°C with a cocktail of chicken anti-GFP (1:8000; Abcam), guinea pig anti-synaptophysin1 (1:800 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized with 0.1% Triton X-100 in PBS for 10 min at RT and stained overnight at 4°C with the appropriate primary antibodies (rabbit anti-ZMPSTE24, Abcam ab38450, 1:100 or mouse monoclonal anti-HA, Abcam ab18181 ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBS-T with 5% skim milk powder at 4°C overnight and incubated in 1:8000 diluted anti-GFP antibody (Ab290; Abcam) in TBS-T with 5% skim milk powder for one hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... and stained with the following primary antibodies by incubating at 4 °C overnight: RECA-1 (1:50, ab9774, mouse, Abcam), α-SMA (1:200 ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% Triton X-100 in PBS and stained overnight at 4°C with ACE2 protein-specific antibody (Abcam Ab15348). Cells were then incubated with anti-rabbit secondary antibody (Alexa Fluor 536 anti-rabbit ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight at 4°C with primary antibodies diluted in Intercept 0.05% Tween-20 as follows: anti-ATP5A1 (Abcam; ab14748), anti-Citrate synthase (Abcam ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoblotting was performed with the following antibodies at 1:2,500–3,000 dilutions for either 1 hour at room temperature or overnight at 4°C: anti-DDDD-K (Abcam, ab1162), anti-α-actin (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature followed by overnight incubation at 4°C with rabbit anti-glutamate receptor 1 (AMPA subtype) phosphoSer 845 antibody (1:500; ab76321, Abcam), rabbit anti-glutamate receptor 1 (AMPA subtype ...