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Citations for abcam :
51 - 100 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... DNA isolation of treated cells was done as per the manufacturer’s instructions stated in Apoptosis DNA Ladder Assay kit purchased from Abcam. After the isolation of genomic DNA from cells exposed to varying doses of personal care products ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... assay was performed on paraffin embedded tissue sections according to the manufacturer’s instructions to detect DNA fragmentation in apoptosis (TUNEL assay kit-HRP-DAB, Cat#: ab206386, Abcam). Briefly ...
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bioRxiv - Immunology 2020Quote: The iNOS recombinant protein (full length) was procured from sigma and Nitric Oxide Synthase activity assay kit was from Abcam. The NOSIP (full length ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Cell Biology 2023Quote: ... pT320-pp1 (1:1000 for the detection of the endogenous protein, 1:30000 for the kinase assay, rabbit, Abcam ab62334), pY15-Cdk1 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Plant Biology 2021Quote: ... The purified TIR proteins (5.0 μM) individually were incubated with the fluorescent DNA probe (25 μM) (Abcam). The samples were measured by microplate reader SynergyH1 (BioTek ...
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bioRxiv - Molecular Biology 2024Quote: AP-sites were tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... the PNBM treated cell lysates were analyzed with a western blot assay to measure protein thio-phosphorylation with the anti-thiophosphate ester rabbit monoclonal antibodies (Abcam, USA).
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bioRxiv - Neuroscience 2022Quote: ... progranulin levels were determined in duplicate using 150 μg of protein lysates per well using a sandwich ELISA assay (abcam, ab252364). Western blot and qPCR analyses were carried out as described above ...
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bioRxiv - Neuroscience 2022Quote: The quantitative detection of serum lipopolysaccharide binding protein (LBP) was performed by Mouse LBP Enzyme-Linked Immunosorbent Assay (ELISA) kit (Abcam, ab213876) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 300μg of protein was used for immunoprecipitation assay using immunoprecipitation kit containing Dynabeads-coupled protein G (magnetic beads) and human anti-MMP9 (1:700) (Abcam, ab38898). Briefly the protein was mixed with anti-MMP9 antibody and incubated overnight at 4°C ...
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bioRxiv - Genetics 2022Quote: ATPase activity of cohesin was measured using EnzChek Phosphate Assay Kit with purified recombinant proteins depleted of free phosphate using Inorganic Phosphate Binding Resin (Abcam: ab270547). Reactions were assembled with10nM cohesin ...
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bioRxiv - Cell Biology 2021Quote: ... Non-specific interactions were blocked for 1 hour with blocking solution (3% BSA, Sigma ref. A9647, 2% donkey serum, Abcam ref. ab7475, 0,3% Triton X-100, PBS). Primary antibodies were diluted in the blocking solution and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody and cellular DNA using DR (ABCAM). Imaging was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... and nuclear DNA-encoded succinate dehydrogenase A (SDH-A) using the MitoBiogenesis In-Cell Enzyme-linked immunosorbent assay (ELISA) Kit (Colorimetric, Abcam, ab110217) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Ubiquitination assay was performed with Ubiquitylation Assay Kit (abcam; ab139467) using recombinant carrier free ACE2 (# 933-ZN-010 ...
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bioRxiv - Cancer Biology 2022Quote: ... the colorimetric assay was performed using acetate assay kit (Abcam), according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: MTT assay was performed using the MTT assay kit (Abcam) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: Comet assay was performed using Comet Assay Kit (Abcam, ab238544) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... end-stage bulk tumor and 4- to 15-weeks control HDTVi liver protein lysates (50 μg) were analyzed using Fluorometric Caspase-1 Assay Kit (Abcam, cat.no. ab273268) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Detection of thiophosphate ester tagged proteins from the in vitro kinase assay was done with a specific anti-thiophosphate ester antibody (#ab92570 Abcam 1:10000). Antibody against total CDC-42 was use for the loading control (#sc-390210 ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Arginase assay (Abcam) activity of control (n = 6) ...
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bioRxiv - Biochemistry 2021Quote: ... and stained for SARS-CoV-2 N protein using Alexa488-labelled-CR3009 antibody and cellular DNA using DRAQ7 (Abcam). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Cancer Biology 2021Quote: Comet assay was performed with the Comet Assay Kit (Abcam, ab238544), following the protocol from the manufacture ...
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bioRxiv - Molecular Biology 2022Quote: Tube formation assay was performed using the angiogenesis assay kit (Abcam). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were then processed through an ELISA based kit for detecting p65 (NF-κB p65 Transcription Factor Assay Kit, abcam, Cambridge, MA, #ab133112). Absorbance at 450 nm was read with SpectraMax iD3 plate reader (Molecular Devices ...
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bioRxiv - Bioengineering 2021Quote: ... and stained for SARS-CoV2 N protein using Alexa488-labelled-CR3009 antibody produced custom (see section for Recombinant mAb production) and cellular DNA using DRAQ7 (ABCAM). Whole-well imaging at 5x was carried out using an Opera Phenix (Perkin Elmer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The genomic DNA crossed with Foxo1a protein was immunoprecipitated by using 5 μg Anti-6×His tag antibody (abcam, ab213204). Antibody against lgG was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: TUNEL stain assay was evaluated with BrdU-Red TUNEL assay kit (Abcam) according to the manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we used a calcium assay kit (Abcam Calcium Assay Kit ab102505, USA). After removing the tissue from PBS ...
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bioRxiv - Pathology 2021Quote: Arginase activity assays were performed using a colorimetric assay (Abcam cat. #180877) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Cell Biology 2021Quote: This assay was performed using an ADP/ATP ratio assay kit (Abcam) following manufacturer ‘s instructions.
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bioRxiv - Biochemistry 2021Quote: Assay was carried out with the Aconitase Activity Assay Kit (Abcam ab109712). Cell lines were generated as indicated and each condition was collected in triplicates of 1,000,000 cells each ...
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bioRxiv - Cancer Biology 2020Quote: ... TUNEL assay was performed using TUNEL Assay Kit - HRP-DAB (Abcam-ab206386) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Apoptosis assays were conducted using the Apoptosis/Necrosis Assay kit (Abcam, #ab176749)18 ...
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bioRxiv - Molecular Biology 2024Quote: ... ATP assay and L-lactate assay were purchased from Abcam (Waltham, MA). PKAα/β/γ subunits antibodies were obtained from Santa-Cruz Biotechnology Inc ...
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bioRxiv - Bioengineering 2020Quote: ... MTS Assay Kit (Abcam) was used to assess hiPSC cardiomyocytes viability.
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bioRxiv - Bioengineering 2020Quote: ... MTS Assay Kit (Abcam) was used to assess hiPSC cardiomyocytes viability.
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bioRxiv - Cancer Biology 2021Quote: ... an MTT assay (Abcam) was carried out according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... TUNEL assay (Abcam, ab206386), using either methyl green or hematoxylin as counterstain ...
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bioRxiv - Molecular Biology 2021Quote: ... MTS assay (Abcam, ab197010) was performed for 2.5 hours and the absorbance was measured at 490 nm ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Genomics 2021Quote: ... Fragmented DNA was then diluted 10 times with ChIP dilution buffer and protein-DNA complexes were captured using an H3K27ac antibody (Abcam AB4729). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... which was performed overnight at 4°C by incubating the shared DNA-protein complex with 10 µg of anti-ADNP (Abcam Ab231950) antibody ...
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bioRxiv - Cell Biology 2022Quote: This assay was performed using LysoLive Lysosomal Acid Lipase Assay Kit (Abcam, ab253380). Briefly ...
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bioRxiv - Immunology 2019Quote: ... colorimetric assays (Cholesterol Quantification kit, #MAK043, Sigma Aldrich; Triglyceride Assay kit, #ab65336, Abcam) were used.