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Citations for abcam :
51 - 100 of 117 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus was produced with 3rd Generation Packaging System Mix (Abcam: LV053) in 293T cells transfected with Lipofectamine 3000 Reagent (Thermo-Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP-qPCR was performed as previously described utilizing 2 μg of anti-H3K27ac (Abcam ab4729) or YY1 (Active Motif 61780)(41) ...
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bioRxiv - Genetics 2020Quote: ... that were detected by SySy antibody and also has signal from Abcam antibody but below the cutoff to be called peaks by MACS2 (Zhang et al., 2008), and low confidence m6A set (ConfGroup3, n=6319) that were only detected by Abcam antibody and almost no signal from SySy antibody ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... Nuclei from one striatum (~10,000) were washed 2X and then resuspended in Digitonin Buffer with either anti-H3K4me3 (Antibody 1: Abcam, Ab8580 (Figure 2); Antibody 2 ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in 2x SSC (3 x 5 min) and incubated in blocking solution containing secondary antibody (anti-chicken Alexa 488, Abcam, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2019Quote: Lysosomal quantity was measured using the Lysosomal Staining Kit – Green Fluorescence – Cytopainter (Abcam). Cells were plated at 60,000 cells per well of a 96 well plate ...
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bioRxiv - Biochemistry 2019Quote: ATPase kit containing malachite green and ammonium molybdate was purchased from Abcam (ab65622). For assays in Figure 4b ...
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bioRxiv - Genetics 2019Quote: ... For mitochondria identification a stock of Mitochondrial Staining Reagent-Green (ab176830, Cytopainter, Abcam) was diluted 1:500 in H2O and 3 µl added to the colonies ...
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bioRxiv - Developmental Biology 2023Quote: ... were incubated with the cell tracking dye Cytopainter Green (ab138891, Abcam, 1:1000) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 μL hydrogel ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... or green fluorescent protein (chicken anti-GFP; Abcam, Cambridge, UK, ab13970, 1:1000). Primary antibody incubation was 16h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... was performed by flow cytometry and triglyceride release assay was determined in supernatant after low-speed centrifugation (300 g, 10 min) using Triglyceride Assay kit (Abcam ab65336). GMPs were collected in a supernatant of granulocytes (3-5 x 106/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against green fluorescent protein (GFP, 1:2000, Abcam, USA), and mCherry (1:2000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Safranin O-fast green staining and immunohistochemical staining for COL2A1 (1:1000, Abcam, USA) were performed according to instruction manual ...
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bioRxiv - Neuroscience 2019Quote: The following primary antibodies were used: chicken anti-green fluorescent protein (1:2000, Abcam), guinea pig anti-Sox6 (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was applied at a 1:1,000 dilution with 3 μM Nuclear Green DCS1 (Abcam) for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 (green)/Alexa Fluor 594 (red) conjugated secondary antibody (Abcam, Cambridge, MA) were then used to detect primary antibodies for 1h ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 (green)/Alexa Fluor 594 (red) conjugated secondary antibody (Abcam, Cambridge, MA) were then used to detect primary antibodies for 1h.
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bioRxiv - Neuroscience 2023Quote: ... GSH and GSSG levels were measured by a green fluorescence assay kit (Abcam, ab205811).
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bioRxiv - Developmental Biology 2024Quote: ... were incubated with the cell tracking dye Cytopainter Green (1:500, cat# ab138891, Abcam) for 30 minutes and then were washed and seeded at a concentration of 100 cells per 20 uL hydrogel ...
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bioRxiv - Cancer Biology 2023Quote: CUT&RUN experiments were performed using a modified protocol for low cell numbers as described in (25) using the following antibodies: anti-TFE3 (ab93808, Abcam, 1:2000), anti-H3K4me3 (monoclonal antibody G.532.8 ...
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bioRxiv - Genomics 2023Quote: ... The following commercial control qPCR assays were also used: GAPDH positive control ChIP primer pair (Abcam ab267832), Human Positive Control Primer Set MYT1 (Active Motif ...
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bioRxiv - Biochemistry 2020Quote: ... Free intracellular Ca2+ was detected using a green-fluorescent Fluo-8 indicator (Abcam, Cat# Ab112129), λ~490nm excitation ...
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bioRxiv - Physiology 2022Quote: ... and/or chicken anti-green fluorescent protein (GFP) (1:500; #ab13970—Abcam, Inc., Cambridge, UK) primary antibody in blocking solution for 24 hours at room temperature in a humid chamber on a gentle rotator plate (GFP antibody also detects YFP) ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin samples were incubated overnight at a low temperature with 10 µg ChIP-grade anti-RNA polymerase II (ab26721; Abcam, Boston, MA, USA) and anti-VDR (sc-1008x ...
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bioRxiv - Genomics 2022Quote: ... The Rabbit IgG control antibodies for ChIP-qPCR experiments were ab172730 (abcam, GR3298823-2, 2ug per 80ug chromatin), and Normal IgG Rabbit 2729 (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: ... slides are incubated with a primary antibody mix with anti-FTL (1:100, Abcam), anti-Aβ (17-24 ...
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bioRxiv - Immunology 2022Quote: ... to visualize nuclei or with lysosomal staining reagents (Green - Cytopainter ab176826 or NIR - Cytopainter ab176824, Abcam). For image analysis and fluorescence intensity profile we used Zen 3.2 software ...
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bioRxiv - Cell Biology 2024Quote: ... fixed cells were given the staining solution mix as recommended by the manufacturer (Abcam, ab65351). However ...
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bioRxiv - Cancer Biology 2021Quote: Primary antibody used in this study was: chicken anti-green fluorescent protein (GFP) (1:1000; Abcam ab13970); Secondary antibodies were ...
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bioRxiv - Cell Biology 2022Quote: ... Primary antibody was anti-green fluorescent protein (mouse anti-GFP, Abcam, Cambridge, UK, Ab291-50, 1:1000). The secondary antibody was anti-mouse IgG horseradish peroxidase conjugate (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by normal serum blocking and stained with anti-Green Florescence Protein (GFP) (Abcam, ab6673, Cambridge, MA), anti-TNF-α (Beijing Biosynthesis Biotechnology Co. ...
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bioRxiv - Neuroscience 2023Quote: ... the primary antibodies used were green fluorescent protein (GFP) (1:100 dilution; Abcam, Cambridge, UK; Cat. #ab183734), neuronal nuclei (NeuN ...
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bioRxiv - Developmental Biology 2022Quote: ... “STAT1 Ab1” consisted of a 50:50 mix of rabbit anti-STAT1 [EPR21057-141] (Abcam #ab234400) and rabbit anti-STAT1 [EPR23049-111] (Abcam ab#239360) ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody mix consisted of donkey anti-mouse 1:1000 (Alexa Fluor 488 ab150117, Abcam), donkey anti rabbit 1:1000 (Cy3 ...
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bioRxiv - Cell Biology 2022Quote: ... Marker antibodies were detected by Alexa fluor® green rabbit or mouse secondary antibodies (Abcam, supplementary table 6). Co- localization of SaCas9 with desmin or albumin markers in liver tissue was detected by Alexa fluor® secondary antibodies (supplementary table S7) ...
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bioRxiv - Microbiology 2020Quote: ... Cloned IRE1-/- MEF cells were validated by RT-qPCR using specific primers and by immunoblot using IRE1-specific antibody (Abcam-ab37073). IRE1-/- cells were complemented with pcDNA3-derived vectors containing WT or the indicated mutant versions of IRE1 ...
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bioRxiv - Neuroscience 2023Quote: ... 50µl of reaction mix (47µl Glucose uptake assay buffer, 2µl of enzyme mix and 1µl of PicoProbe, all supplied by Abcam) was added to each sample and standard well ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-biotin antibodies were added (equal mix of Bethyl laboratories, no. 150-109A, and Abcam no. Ab53494), 30 µg of each antibody per 60 µL of bead slurry ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and secondaries antibody mix containing goat anti-rabbit IgG H&L (Alexa Fluor® 488) (Abcam ab6702) (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96 well plates were stained using Janus Green cell normalization stain kit (Abcam, # ab111622). OD value at 595 nm was measured by Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight in primary antibodies against green- fluorescent protein (GFP, 1:1000 in antibody diluent, Abcam, ab13970), calcitonin gene-related peptide (CGRP ...
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bioRxiv - Neuroscience 2023Quote: ... cells grown in the same 96-well plate were stained using the Janus Green Cell Normalization Staining Kit (Abcam). OD values at 595 nm were measured by a Labsystems Multiskan Bichromatic plate reader (Titertek Instruments ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... hCMEC/D3 and primary rodent microglia cells were labeled with Cell Tracking Dye Kit - Green - Cytopainter (ab138891, Abcam, Cambridge, UK) and Cell Tracking Dye Kit - Deep Red - Cytopainter (ab138894 ...
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bioRxiv - Microbiology 2021Quote: ... for 2 hours after which the cells were washed and stained with 1x Apoxin Green Indicator and 1x 7-AAD (Apoptosis/Necrosis detection kit, Abcam) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: PPGYFP NTS tissue was processed free-floating and incubated with chicken anti-green fluorescent protein (GFP; AbCam, ab13970, 1:1000) and/or rabbit anti-c-FOS (Calbiochem ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was replaced with 200 μL DMEM and then incubated with a mixture of 2 μL Apopxin Green (Abcam) and 0.4 μL Propidium Iodide (15 mM ...
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bioRxiv - Microbiology 2020Quote: ... T cells were detected in sections from TgBAC(lck:EGFP)vcc4 zebrafish by anti-GFP staining to enhance visible fluorescent green signal (primary antibody: ab13970, Abcam; secondary antibody: ab150173, Abcam), stained slides were then mounted with coverslips using Fluoromount G containing DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...